Colorado PROFILES, The Colorado Clinical and Translational Sciences Institute (CCTSI)
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Last Name
Institution

Derek L Bolhuis

TitlePost-Doctoral Fellow
InstitutionUniversity of Colorado Denver - Anschutz Medical Campus
DepartmentSOM-BioChem&Molecular Genetics

    Collapse Bibliographic 
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    Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Faculty can login to make corrections and additions.
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    1. Yang D, Wang X, Liu J, Gong Y, Nair P, Sun J, Qian X, Cui J, Zeng H, Dong A, Harding RJ, Burgess-Brown N, Beyett TS, Song D, Krause HM, Diamond ML, Bolhuis DL, Brown NG, Arrowsmith CH, Edwards AM, Halabelian L, Peng H. High-throughput protein target mapping enables accelerated bioactivity discovery for ToxCast and PFAS compounds. Proc Natl Acad Sci U S A. 2026 Jun 23; 123(25):e2515940123. PMID: 42247526.
      View in: PubMed
    2. Bukhari Z, Gu L, Nederstigt AE, Cope LJ, Bolhuis DL, Harvey K, Allen T, Hill S, Yang Y, Lawson G, Lu C, Tran T, Pineda L, Low L, Chiang A, Song J, Fong MV, Rangel VM, Chan WK, Kleiger G, Goldfarb D, Vierra CA, Brown NG, Harrison JS. Linked-domain inhibitors designed to block UBE2D induce the unfolded protein response. Cell Chem Biol. 2025 Dec 18; 32(12):1532-1544.e7. PMID: 41352346.
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    3. Paul D, Bolhuis DL, Yan H, Das S, Xu X, Abbate CC, Jenkins LMM, Emanuele MJ, Andresson T, Huang J, Albeck JG, Brown NG, Cappell SD. Transient APC/C inactivation by mTOR boosts glycolysis during cell cycle entry. Nature. 2025 10; 646(8083):198-207. PMID: 40739344.
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    4. Bonacci T, Bolhuis DL, Brown NG, Emanuele MJ. Mechanisms of USP18 specificity toward ISG15 revealed by paralog sequence analysis comparison. J Biol Chem. 2025 07; 301(7):110288. PMID: 40436319.
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    5. Bolhuis DL, Fleifel D, Bonacci T, Wang X, Mouery BL, Cook JG, Brown NG, Emanuele MJ. USP37 prevents unscheduled replisome unloading through MCM complex deubiquitination. Nat Commun. 2025 May 16; 16(1):4575. PMID: 40379725.
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    6. Yang D, Wang X, Liu J, Gong Y, Nair P, Sun J, Qian X, Cui C, Zeng H, Dong A, Harding RJ, Burgess-Brown N, Beyett TS, Song D, Krause H, Diamond ML, Bolhuis DL, Brown NG, Arrowsmith CH, Edwards AM, Halabelian L, Peng H. High-throughput protein target mapping enables accelerated bioactivity discovery for ToxCast and PFAS compounds. bioRxiv. 2025 Mar 25. PMID: 40196539.
      View in: PubMed
    7. Bolhuis DL, Fleifel D, Bonacci T, Wang X, Mouery BL, Cook JG, Brown NG, Emanuele MJ. USP37 prevents unscheduled replisome unloading through MCM complex deubiquitination. bioRxiv. 2024 Sep 03. PMID: 39282338.
      View in: PubMed
    8. Bukhari Z, Gu L, Nederstigt AE, Cope LJ, Bolhuis DL, Harvey K, Allen T, Hill S, Yang Y, Lawson G, Lu C, Tran T, Pineda L, Low L, Chiang A, Song J, Fong MV, Rangel VM, Chan WK, Kleiger G, Goldfarb D, Vierra CA, Brown NG, Harrison JS. Design of linked-domain protein inhibitors of UBE2D as tools to study cellular ubiquitination. bioRxiv. 2024 Sep 02. PMID: 39282319.
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    9. Mouery RD, Lukasik K, Hsu C, Bonacci T, Bolhuis DL, Wang X, Mills CA, Toomer ED, Canterbury OG, Robertson KC, Branigan TB, Brown NG, Herring LE, Gupton SL, Emanuele MJ. Proteomic analysis reveals a PLK1-dependent G2/M degradation program and a role for AKAP2 in coordinating the mitotic cytoskeleton. Cell Rep. 2024 08 27; 43(8):114510. PMID: 39018246.
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    10. Bonacci T, Bolhuis DL, Brown NG, Emanuele MJ. Mechanisms of USP18 deISGylation revealed by comparative analysis with its human paralog USP41. bioRxiv. 2024 May 28. PMID: 38853827.
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    11. Fan Y, Bilkey N, Bolhuis DL, Slep KC, Dixit R. A divergent tumor overexpressed gene domain and oligomerization contribute to SPIRAL2 function in stabilizing microtubule minus ends. Plant Cell. 2024 Mar 29; 36(4):1056-1071. PMID: 38011314.
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    12. Lu X, Chandravanshi M, Sabbasani VR, Gaikwad S, Hughitt VK, Gyabaah-Kessie N, Scroggins BT, Das S, Myint W, Clapp ME, Schwieters CD, Dyba MA, Bolhuis DL, Koscielniak JW, Andresson T, Emanuele MJ, Brown NG, Matsuo H, Chari R, Citrin DE, Mock BA, Swenson RE, Walters KJ. A structure-based designed small molecule depletes hRpn13Pru and a select group of KEN box proteins. Nat Commun. 2024 Mar 20; 15(1):2485. PMID: 38509117.
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    13. Bolhuis DL, Emanuele MJ, Brown NG. Friend or foe? Reciprocal regulation between E3 ubiquitin ligases and deubiquitinases. Biochem Soc Trans. 2024 02 28; 52(1):241-267. PMID: 38414432.
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    14. Bolhuis DL, Dixit R, Slep KC. Crystal structure of the Arabidopsis SPIRAL2 C-terminal domain reveals a p80-Katanin-like domain. PLoS One. 2023; 18(12):e0290024. PMID: 38157339.
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    15. Ledvin L, Gassaway BM, Tawil J, Urso O, Pizzo D, Welsh KA, Bolhuis DL, Fisher D, Bonni A, Gygi SP, Brown NG, Ferguson CJ. The anaphase-promoting complex controls a ubiquitination-phosphoprotein axis in chromatin during neurodevelopment. Dev Cell. 2023 12 04; 58(23):2666-2683.e9. PMID: 37875116.
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    16. Mouery RD, Hsu C, Bonacci T, Bolhuis DL, Wang X, Mills CA, Toomer ED, Canterbury OG, Robertson KC, Branigan TB, Brown NG, Herring LE, Emanuele MJ. Proteomic Analysis Reveals a PLK1-Dependent G2/M Degradation Program and Links PKA-AKAP2 to Cell Cycle Control. bioRxiv. 2023 Oct 12. PMID: 37873169.
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    17. Bodrug T, Welsh KA, Bolhuis DL, Paul?konis E, Martinez-Chacin RC, Liu B, Pinkin N, Bonacci T, Cui L, Xu P, Roscow O, Amann SJ, Grishkovskaya I, Emanuele MJ, Harrison JS, Steimel JP, Hahn KM, Zhang W, Zhong ED, Haselbach D, Brown NG. Time-resolved cryo-EM (TR-EM) analysis of substrate polyubiquitination by the RING E3 anaphase-promoting complex/cyclosome (APC/C). Nat Struct Mol Biol. 2023 11; 30(11):1663-1674. PMID: 37735619.
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    18. Hodáková Z, Grishkovskaya I, Brunner HL, Bolhuis DL, Belacic K, Schleiffer A, Kotisch H, Brown NG, Haselbach D. Cryo-EM structure of the chain-elongating E3 ubiquitin ligase UBR5. EMBO J. 2023 08 15; 42(16):e113348. PMID: 37409633.
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    19. Chen J, Bolhuis DL, Laggner C, Kong D, Yu L, Wang X, Emanuele MJ, Brown NG, Liu P. AtomNet-Aided OTUD7B Inhibitor Discovery and Validation. Cancers (Basel). 2023 Jan 14; 15(2). PMID: 36672466.
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    20. Iskandar SE, Pelton JM, Wick ET, Bolhuis DL, Baldwin AS, Emanuele MJ, Brown NG, Bowers AA. Enabling Genetic Code Expansion and Peptide Macrocyclization in mRNA Display via a Promiscuous Orthogonal Aminoacyl-tRNA Synthetase. J Am Chem Soc. 2023 01 25; 145(3):1512-1517. PMID: 36630539.
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    21. Bolhuis DL, Martinez-Chacin RC, Welsh KA, Bodrug T, Cui L, Emanuele MJ, Brown NG. Examining the mechanistic relationship of APC/CCDH1 and its interphase inhibitor EMI1. Protein Sci. 2022 06; 31(6):e4324. PMID: 35634770.
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    22. Kandolf S, Grishkovskaya I, Belacic K, Bolhuis DL, Amann S, Foster B, Imre R, Mechtler K, Schleiffer A, Tagare HD, Zhong ED, Meinhart A, Brown NG, Haselbach D. Cryo-EM structure of the plant 26S proteasome. Plant Commun. 2022 05 09; 3(3):100310. PMID: 35576154.
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    23. Welsh KA, Bolhuis DL, Nederstigt AE, Boyer J, Temple BRS, Bonacci T, Gu L, Ordureau A, Harper JW, Steimel JP, Zhang Q, Emanuele MJ, Harrison JS, Brown NG. Functional conservation and divergence of the helix-turn-helix motif of E2 ubiquitin-conjugating enzymes. EMBO J. 2022 02 01; 41(3):e108823. PMID: 34942047.
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    24. Franks JL, Martinez-Chacin RC, Wang X, Tiedemann RL, Bonacci T, Choudhury R, Bolhuis DL, Enrico TP, Mouery RD, Damrauer JS, Yan F, Harrison JS, Major MB, Hoadley KA, Suzuki A, Rothbart SB, Brown NG, Emanuele MJ. In silico APC/C substrate discovery reveals cell cycle-dependent degradation of UHRF1 and other chromatin regulators. PLoS Biol. 2020 12; 18(12):e3000975. PMID: 33306668.
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    25. Martinez-Chacin RC, Bodrug T, Bolhuis DL, Kedziora KM, Bonacci T, Ordureau A, Gibbs ME, Weissmann F, Qiao R, Grant GD, Cook JG, Peters JM, Wade Harper J, Emanuele MJ, Brown NG. Ubiquitin chain-elongating enzyme UBE2S activates the RING E3 ligase APC/C for substrate priming. Nat Struct Mol Biol. 2020 06; 27(6):550-560. PMID: 32393902.
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    26. Watson ER, Grace CRR, Zhang W, Miller DJ, Davidson IF, Prabu JR, Yu S, Bolhuis DL, Kulko ET, Vollrath R, Haselbach D, Stark H, Peters JM, Brown NG, Sidhu SS, Schulman BA. Protein engineering of a ubiquitin-variant inhibitor of APC/C identifies a cryptic K48 ubiquitin chain binding site. Proc Natl Acad Sci U S A. 2019 08 27; 116(35):17280-17289. PMID: 31350353.
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