Colorado PROFILES, The Colorado Clinical and Translational Sciences Institute (CCTSI)
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Last Name
Institution

Deborah S Wuttke

TitleProfessor
InstitutionUniversity of Colorado Boulder
DepartmentBiochemistry
Phone303/492-4576

    Collapse Research 
    Collapse research activities and funding
    R01GM139274     (WUTTKE, DEBORAH S.)Jul 7, 2020 - May 31, 2025
    NIH
    RPA and RPA-like Complexes at Telomeres
    Role: Principal Investigator
    R01GM120347     (WUTTKE, DEBORAH S.)Sep 22, 2016 - Jun 30, 2025
    NIH
    RNA Regulation of Transcription Factor Activity
    Role: Principal Investigator
    S10RR028036     (WUTTKE, DEBORAH S.)Apr 15, 2010 - Apr 14, 2011
    NIH
    Purchase of a CD Spectropolarimeter
    Role: Principal Investigator
    R21NS059370     (WUTTKE, DEBORAH S.)Mar 5, 2007 - Feb 28, 2009
    NIH
    Development of a HTS Assay Targeting End Protection of Telomeres
    Role: Principal Investigator
    P41GM068928     (WUTTKE, DEBORAH S.)Jul 15, 2003 - Apr 30, 2012
    NIH
    Purchase of 900 MHz NMR Spectrometer
    Role: Principal Investigator
    R01GM059414     (WUTTKE, DEBORAH S.)May 1, 1999 - May 31, 2018
    NIH
    Single Stranded DNA Recognition in Telomeres
    Role: Principal Investigator

    Collapse Bibliographic 
    Collapse selected publications
    Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Faculty can login to make corrections and additions.
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    1. Kletzien OA, Wuttke DS, Batey RT. The RNA-binding domain of hnRNP U extends beyond the RGG/RG motifs. bioRxiv. 2023 Sep 20. PMID: 37786719.
      View in: PubMed
    2. Lammer NC, Allen MA, Batey RT, Wuttke DS. Quantification of transcriptome changes to investigate the role of glucocorticoid receptor-RNA binding during dexamethasone treatment. BMC Res Notes. 2023 Aug 22; 16(1):181. PMID: 37608297.
      View in: PubMed
    3. Olson CL, Barbour AT, Wieser TA, Wuttke DS. RPA engages telomeric G-quadruplexes more effectively than CST. Nucleic Acids Res. 2023 06 09; 51(10):5073-5086. PMID: 37140062.
      View in: PubMed
    4. Lammer NC, Ashraf HM, Ugay DA, Spencer SL, Allen MA, Batey RT, Wuttke DS. RNA binding by the glucocorticoid receptor attenuates dexamethasone-induced gene activation. Sci Rep. 2023 06 09; 13(1):9385. PMID: 37296231.
      View in: PubMed
    5. Barbour AT, Wuttke DS. RPA-like single-stranded DNA-binding protein complexes including CST serve as specialized processivity factors for polymerases. Curr Opin Struct Biol. 2023 08; 81:102611. PMID: 37245465.
      View in: PubMed
    6. Hamilton DJ, Hein AE, Wuttke DS, Batey RT. The DNA binding high mobility group box protein family functionally binds RNA. Wiley Interdiscip Rev RNA. 2023 Sep-Oct; 14(5):e1778. PMID: 36646476.
      View in: PubMed
    7. Wieser TA, Wuttke DS. Replication Protein A Utilizes Differential Engagement of Its DNA-Binding Domains to Bind Biologically Relevant ssDNAs in Diverse Binding Modes. Biochemistry. 2022 11 15; 61(22):2592-2606. PMID: 36278947.
      View in: PubMed
    8. Steiner HR, Lammer NC, Batey RT, Wuttke DS. An Extended DNA Binding Domain of the Estrogen Receptor Alpha Directly Interacts with RNAs in Vitro. Biochemistry. 2022 11 15; 61(22):2490-2494. PMID: 36239332.
      View in: PubMed
    9. Olson CL, Barbour AT, Wuttke DS. Filling in the blanks: how the C-strand catches up to the G-strand at replicating telomeres using CST. Nat Struct Mol Biol. 2022 08; 29(8):730-733. PMID: 35948770.
      View in: PubMed
    10. Hamilton DJ, Hein AE, Holmes ZE, Wuttke DS, Batey RT. The DNA-Binding High-Mobility Group Box Domain of Sox Family Proteins Directly Interacts with RNA In Vitro. Biochemistry. 2022 May 05. PMID: 35511045.
      View in: PubMed
    11. Zaug AJ, Lim CJ, Olson CL, Carilli MT, Goodrich KJ, Wuttke DS, Cech TR. CST does not evict elongating telomerase but prevents initiation by ssDNA binding. Nucleic Acids Res. 2021 11 18; 49(20):11653-11665. PMID: 34718732.
      View in: PubMed
    12. Lloyd NR, Wuttke DS. Cyp33 binds AU-rich RNA motifs via an extended interface that competitively disrupts the gene repressive Cyp33-MLL1 interaction in vitro. PLoS One. 2021; 16(2):e0237956. PMID: 33606679.
      View in: PubMed
    13. Nakamoto MY, Lammer NC, Batey RT, Wuttke DS. hnRNPK recognition of the B motif of Xist and other biological RNAs. Nucleic Acids Res. 2020 09 18; 48(16):9320-9335. PMID: 32813011.
      View in: PubMed
    14. Lim CJ, Barbour AT, Zaug AJ, Goodrich KJ, McKay AE, Wuttke DS, Cech TR. The structure of human CST reveals a decameric assembly bound to telomeric DNA. Science. 2020 06 05; 368(6495):1081-1085. PMID: 32499435.
      View in: PubMed
    15. Carter AC, Xu J, Nakamoto MY, Wei Y, Zarnegar BJ, Shi Q, Broughton JP, Ransom RC, Salhotra A, Nagaraja SD, Li R, Dou DR, Yost KE, Cho SW, Mistry A, Longaker MT, Khavari PA, Batey RT, Wuttke DS, Chang HY. Spen links RNA-mediated endogenous retrovirus silencing and X chromosome inactivation. Elife. 2020 05 07; 9. PMID: 32379046.
      View in: PubMed
    16. Holmes ZE, Hamilton DJ, Hwang T, Parsonnet NV, Rinn JL, Wuttke DS, Batey RT. The Sox2 transcription factor binds RNA. Nat Commun. 2020 04 14; 11(1):1805. PMID: 32286318.
      View in: PubMed
    17. Nakamoto MY, Rudolph J, Wuttke DS, Luger K. Nonspecific Binding of RNA to PARP1 and PARP2 Does Not Lead to Catalytic Activation. Biochemistry. 2019 12 24; 58(51):5107-5111. PMID: 31829559.
      View in: PubMed
    18. Parsonnet NV, Lammer NC, Holmes ZE, Batey RT, Wuttke DS. The glucocorticoid receptor DNA-binding domain recognizes RNA hairpin structures with high affinity. Nucleic Acids Res. 2019 09 05; 47(15):8180-8192. PMID: 31147715.
      View in: PubMed
    19. Lewis KA, Altschuler SE, Wuttke DS. Measuring Low-Picomolar Apparent Binding Affinities by Minigel Electrophoretic Mobility Shift. Methods Mol Biol. 2019; 1855:341-354. PMID: 30426430.
      View in: PubMed
    20. Glustrom LW, Lyon KR, Paschini M, Reyes CM, Parsonnet NV, Toro TB, Lundblad V, Wuttke DS. Single-stranded telomere-binding protein employs a dual rheostat for binding affinity and specificity that drives function. Proc Natl Acad Sci U S A. 2018 10 09; 115(41):10315-10320. PMID: 30249661.
      View in: PubMed
    21. Lloyd NR, Wuttke DS. Discrimination against RNA Backbones by a ssDNA Binding Protein. Structure. 2018 05 01; 26(5):722-733.e2. PMID: 29681468.
      View in: PubMed
    22. McKercher MA, Guan X, Tan Z, Wuttke DS. Diversity in peptide recognition by the SH2 domain of SH2B1. Proteins. 2018 02; 86(2):164-176. PMID: 29127727.
      View in: PubMed
    23. Hom RA, Wuttke DS. Human CST Prefers G-Rich but Not Necessarily Telomeric Sequences. Biochemistry. 2017 08 15; 56(32):4210-4218. PMID: 28726394.
      View in: PubMed
    24. McKercher MA, Guan X, Tan Z, Wuttke DS. Multimodal Recognition of Diverse Peptides by the C-Terminal SH2 Domain of Phospholipase C-?1 Protein. Biochemistry. 2017 04 25; 56(16):2225-2237. PMID: 28376302.
      View in: PubMed
    25. McKercher MA, Wuttke DS. Calorimetric Measurement of SH2 Domain Ligand Affinities. Methods Mol Biol. 2017; 1555:291-305. PMID: 28092039.
      View in: PubMed
    26. McKercher MA, Wuttke DS. NMR Chemical Shift Mapping of SH2 Peptide Interactions. Methods Mol Biol. 2017; 1555:269-290. PMID: 28092038.
      View in: PubMed
    27. Lloyd NR, Dickey TH, Hom RA, Wuttke DS. Tying up the Ends: Plasticity in the Recognition of Single-Stranded DNA at Telomeres. Biochemistry. 2016 09 27; 55(38):5326-40. PMID: 27575340.
      View in: PubMed
    28. Pinzaru AM, Hom RA, Beal A, Phillips AF, Ni E, Cardozo T, Nair N, Choi J, Wuttke DS, Sfeir A, Denchi EL. Telomere Replication Stress Induced by POT1 Inactivation Accelerates Tumorigenesis. Cell Rep. 2016 06 07; 15(10):2170-2184. PMID: 27239034.
      View in: PubMed
    29. Lloyd NR, Wuttke DS. Less is more: structures of difficult targets with minimal constraints. Structure. 2014 Sep 02; 22(9):1223-1224. PMID: 25185825.
      View in: PubMed
    30. Dickey TH, Wuttke DS. The telomeric protein Pot1 from Schizosaccharomyces pombe binds ssDNA in two modes with differing 3' end availability. Nucleic Acids Res. 2014 Sep; 42(15):9656-65. PMID: 25074378.
      View in: PubMed
    31. Rao T, Lubin JW, Armstrong GS, Tucey TM, Lundblad V, Wuttke DS. Structure of Est3 reveals a bimodal surface with differential roles in telomere replication. Proc Natl Acad Sci U S A. 2014 Jan 07; 111(1):214-8. PMID: 24344315.
      View in: PubMed
    32. Lewis KA, Pfaff DA, Earley JN, Altschuler SE, Wuttke DS. The tenacious recognition of yeast telomere sequence by Cdc13 is fully exerted by a single OB-fold domain. Nucleic Acids Res. 2014 Jan; 42(1):475-84. PMID: 24057216.
      View in: PubMed
    33. Dickey TH, Altschuler SE, Wuttke DS. Single-stranded DNA-binding proteins: multiple domains for multiple functions. Structure. 2013 Jul 02; 21(7):1074-84. PMID: 23823326.
      View in: PubMed
    34. Lubin JW, Rao T, Mandell EK, Wuttke DS, Lundblad V. Dissecting protein function: an efficient protocol for identifying separation-of-function mutations that encode structurally stable proteins. Genetics. 2013 Mar; 193(3):715-25. PMID: 23307900.
      View in: PubMed
    35. Altschuler SE, Lewis KA, Wuttke DS. Practical strategies for the evaluation of high-affinity protein/nucleic acid interactions. J Nucleic Acids Investig. 2013 Jan 01; 4(1):19-28. PMID: 25197549.
      View in: PubMed
    36. Dickey TH, McKercher MA, Wuttke DS. Nonspecific recognition is achieved in Pot1pC through the use of multiple binding modes. Structure. 2013 Jan 08; 21(1):121-132. PMID: 23201273.
      View in: PubMed
    37. Altschuler SE, Croy JE, Wuttke DS. A small molecule inhibitor of Pot1 binding to telomeric DNA. Biochemistry. 2012 Oct 09; 51(40):7833-45. PMID: 22978652.
      View in: PubMed
    38. Lewis KA, Wuttke DS. Telomerase and telomere-associated proteins: structural insights into mechanism and evolution. Structure. 2012 Jan 11; 20(1):28-39. PMID: 22244753.
      View in: PubMed
    39. Braun LJ, Eldridge AM, Cummiskey J, Arthur KK, Wuttke DS. The role of adjuvant in mediating antigen structure and stability. J Pharm Sci. 2012 Apr; 101(4):1391-9. PMID: 22213631.
      View in: PubMed
    40. Altschuler SE, Dickey TH, Wuttke DS. Schizosaccharomyces pombe protection of telomeres 1 utilizes alternate binding modes to accommodate different telomeric sequences. Biochemistry. 2011 Sep 06; 50(35):7503-13. PMID: 21815629.
      View in: PubMed
    41. Mandell EK, Gelinas AD, Wuttke DS, Lundblad V. Sequence-specific binding to telomeric DNA is not a conserved property of the Cdc13 DNA binding domain. Biochemistry. 2011 Jul 26; 50(29):6289-91. PMID: 21668015.
      View in: PubMed
    42. Alford JR, Fowler AC, Wuttke DS, Kerwin BA, Latypov RF, Carpenter JF, Randolph TW. Effect of benzyl alcohol on recombinant human interleukin-1 receptor antagonist structure and hydrogen-deuterium exchange. J Pharm Sci. 2011 Oct; 100(10):4215-24. PMID: 21557223.
      View in: PubMed
    43. Lee J, Mandell EK, Rao T, Wuttke DS, Lundblad V. Investigating the role of the Est3 protein in yeast telomere replication. Nucleic Acids Res. 2010 Apr; 38(7):2279-90. PMID: 20047960.
      View in: PubMed
    44. Gelinas AD, Paschini M, Reyes FE, H?roux A, Batey RT, Lundblad V, Wuttke DS. Telomere capping proteins are structurally related to RPA with an additional telomere-specific domain. Proc Natl Acad Sci U S A. 2009 Nov 17; 106(46):19298-303. PMID: 19884503.
      View in: PubMed
    45. Croy JE, Altschuler SE, Grimm NE, Wuttke DS. Nonadditivity in the recognition of single-stranded DNA by the schizosaccharomyces pombe protection of telomeres 1 DNA-binding domain, Pot1-DBD. Biochemistry. 2009 Jul 28; 48(29):6864-75. PMID: 19518131.
      View in: PubMed
    46. Croy JE, Wuttke DS. Insights into the dynamics of specific telomeric single-stranded DNA recognition by Pot1pN. J Mol Biol. 2009 Apr 10; 387(4):935-48. PMID: 19232358.
      View in: PubMed
    47. Zappulla DC, Roberts JN, Goodrich KJ, Cech TR, Wuttke DS. Inhibition of yeast telomerase action by the telomeric ssDNA-binding protein, Cdc13p. Nucleic Acids Res. 2009 Feb; 37(2):354-67. PMID: 19043074.
      View in: PubMed
    48. Alford JR, Kwok SC, Roberts JN, Wuttke DS, Kendrick BS, Carpenter JF, Randolph TW. High concentration formulations of recombinant human interleukin-1 receptor antagonist: I. Physical characterization. J Pharm Sci. 2008 Aug; 97(8):3035-50. PMID: 17973297.
      View in: PubMed
    49. Croy JE, Fast JL, Grimm NE, Wuttke DS. Deciphering the mechanism of thermodynamic accommodation of telomeric oligonucleotide sequences by the Schizosaccharomyces pombe protection of telomeres 1 (Pot1pN) protein. Biochemistry. 2008 Apr 15; 47(15):4345-4358. PMID: 18355038.
      View in: PubMed
    50. Eldridge AM, Wuttke DS. Probing the mechanism of recognition of ssDNA by the Cdc13-DBD. Nucleic Acids Res. 2008 Mar; 36(5):1624-33. PMID: 18250086.
      View in: PubMed
    51. Theobald DL, Wuttke DS. Accurate structural correlations from maximum likelihood superpositions. PLoS Comput Biol. 2008 Feb; 4(2):e43. PMID: 18282091.
      View in: PubMed
    52. Strauss DM, Wuttke DS. Characterization of protein-protein interactions critical for poliovirus replication: analysis of 3AB and VPg binding to the RNA-dependent RNA polymerase. J Virol. 2007 Jun; 81(12):6369-78. PMID: 17409142.
      View in: PubMed
    53. Theobald DL, Wuttke DS. Empirical Bayes hierarchical models for regularizing maximum likelihood estimation in the matrix Gaussian Procrustes problem. Proc Natl Acad Sci U S A. 2006 Dec 05; 103(49):18521-7. PMID: 17130458.
      View in: PubMed
    54. Croy JE, Wuttke DS. Themes in ssDNA recognition by telomere-end protection proteins. Trends Biochem Sci. 2006 Sep; 31(9):516-25. PMID: 16890443.
      View in: PubMed
    55. Croy JE, Podell ER, Wuttke DS. A new model for Schizosaccharomyces pombe telomere recognition: the telomeric single-stranded DNA-binding activity of Pot11-389. J Mol Biol. 2006 Aug 04; 361(1):80-93. PMID: 16842820.
      View in: PubMed
    56. Theobald DL, Wuttke DS. THESEUS: maximum likelihood superpositioning and analysis of macromolecular structures. Bioinformatics. 2006 Sep 01; 22(17):2171-2. PMID: 16777907.
      View in: PubMed
    57. Eldridge AM, Halsey WA, Wuttke DS. Identification of the determinants for the specific recognition of single-strand telomeric DNA by Cdc13. Biochemistry. 2006 Jan 24; 45(3):871-9. PMID: 16411763.
      View in: PubMed
    58. Theobald DL, Wuttke DS. Divergent evolution within protein superfolds inferred from profile-based phylogenetics. J Mol Biol. 2005 Dec 02; 354(3):722-37. PMID: 16266719.
      View in: PubMed
    59. Jacobs SA, Podell ER, Wuttke DS, Cech TR. Soluble domains of telomerase reverse transcriptase identified by high-throughput screening. Protein Sci. 2005 Aug; 14(8):2051-8. PMID: 16046627.
      View in: PubMed
    60. Theobald DL, Wuttke DS. Prediction of multiple tandem OB-fold domains in telomere end-binding proteins Pot1 and Cdc13. Structure. 2004 Oct; 12(10):1877-9. PMID: 15458635.
      View in: PubMed
    61. Mitton-Fry RM, Anderson EM, Theobald DL, Glustrom LW, Wuttke DS. Structural basis for telomeric single-stranded DNA recognition by yeast Cdc13. J Mol Biol. 2004 Apr 23; 338(2):241-55. PMID: 15066429.
      View in: PubMed
    62. Wuttke DS. Targeting the end; the structure of the Ku80 C-terminal domain. Structure. 2004 Mar; 12(3):355-6. PMID: 15016349.
      View in: PubMed
    63. Theobald DL, Cervantes RB, Lundblad V, Wuttke DS. Homology among telomeric end-protection proteins. Structure. 2003 Sep; 11(9):1049-50. PMID: 12962623.
      View in: PubMed
    64. Strauss DM, Glustrom LW, Wuttke DS. Towards an understanding of the poliovirus replication complex: the solution structure of the soluble domain of the poliovirus 3A protein. J Mol Biol. 2003 Jul 04; 330(2):225-34. PMID: 12823963.
      View in: PubMed
    65. Anderson EM, Halsey WA, Wuttke DS. Site-directed mutagenesis reveals the thermodynamic requirements for single-stranded DNA recognition by the telomere-binding protein Cdc13. Biochemistry. 2003 Apr 08; 42(13):3751-8. PMID: 12667066.
      View in: PubMed
    66. Theobald DL, Mitton-Fry RM, Wuttke DS. Nucleic acid recognition by OB-fold proteins. Annu Rev Biophys Biomol Struct. 2003; 32:115-33. PMID: 12598368.
      View in: PubMed
    67. Ojennus DD, Lehto SE, Wuttke DS. Electrostatic interactions in the reconstitution of an SH2 domain from constituent peptide fragments. Protein Sci. 2003 Jan; 12(1):44-55. PMID: 12493827.
      View in: PubMed
    68. Mitton-Fry RM, Anderson EM, Hughes TR, Lundblad V, Wuttke DS. Conserved structure for single-stranded telomeric DNA recognition. Science. 2002 Apr 05; 296(5565):145-7. PMID: 11935027.
      View in: PubMed
    69. Mitton-Fry RM, Wuttke DS. 1H, 13C and 15N resonance assignments of the DNA-binding domain of the essential protein Cdc13 complexed with single-stranded telomeric DNA. J Biomol NMR. 2002 Apr; 22(4):379-80. PMID: 12018489.
      View in: PubMed
    70. Ojennus DD, Fleissner MR, Wuttke DS. Reconstitution of a native-like SH2 domain from disordered peptide fragments examined by multidimensional heteronuclear NMR. Protein Sci. 2001 Nov; 10(11):2162-75. PMID: 11604523.
      View in: PubMed
    71. Dahlke Ojennus D, Mitton-Fry RM, Wuttke DS. Induced alignment and measurement of dipolar couplings of an SH2 domain through direct binding with filamentous phage. J Biomol NMR. 1999 Jun; 14(2):175-9. PMID: 21080256.
      View in: PubMed
    72. Foster MP, Wuttke DS, Clemens KR, Jahnke W, Radhakrishnan I, Tennant L, Reymond M, Chung J, Wright PE. Chemical shift as a probe of molecular interfaces: NMR studies of DNA binding by the three amino-terminal zinc finger domains from transcription factor IIIA. J Biomol NMR. 1998 Jul; 12(1):51-71. PMID: 9729788.
      View in: PubMed
    73. Wuttke DS, Foster MP, Case DA, Gottesfeld JM, Wright PE. Solution structure of the first three zinc fingers of TFIIIA bound to the cognate DNA sequence: determinants of affinity and sequence specificity. J Mol Biol. 1997 Oct 17; 273(1):183-206. PMID: 9367756.
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    74. Foster MP, Wuttke DS, Radhakrishnan I, Case DA, Gottesfeld JM, Wright PE. Domain packing and dynamics in the DNA complex of the N-terminal zinc fingers of TFIIIA. Nat Struct Biol. 1997 Aug; 4(8):605-8. PMID: 9253405.
      View in: PubMed
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