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Marcelo Carlos Sousa

TitleProfessor
InstitutionUniversity of Colorado Boulder
DepartmentBiochemistry
Phone303/735-4341

    Collapse Research 
    Collapse research activities and funding
    R01GM127462     (SOUSA, MARCELO C.)May 1, 2018 - Mar 31, 2022
    NIH
    Mechanism of Transport and Folding of Outer Membrane Proteins
    Role: Principal Investigator
    R01AI080709     (SOUSA, MARCELO C.)May 22, 2009 - Jan 31, 2018
    NIH
    Transport and Insertion of Outer Membrane Proteins
    Role: Principal Investigator
    R01EY019050     (SOUSA, MARCELO C.)May 1, 2009 - Apr 30, 2013
    NIH
    Molecular Mechanism of Retinal Guanylate Cyclase Activation by GCAPs
    Role: Principal Investigator
    R56AI060841     (SOUSA, MARCELO C.)Jul 1, 2004 - Jun 30, 2012
    NIH
    Mechanisms of Resistance in Gram-Negative Bacteria
    Role: Principal Investigator
    R01AI060841     (SOUSA, MARCELO CARLOS)Jul 1, 2004 - Jun 30, 2009
    NIH
    Mechanism of Enzymes Mediating Resistance in Pseudomonas
    Role: Principal Investigator

    Collapse Bibliographic 
    Collapse selected publications
    Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Faculty can login to make corrections and additions.
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    1. Lee Upton S, Tay JW, Schwartz DK, Sousa MC. Similarly slow diffusion of BAM and SecYEG complexes in live E.?coli cells observed with 3D spt-PALM. Biophys J. 2023 11 21; 122(22):4382-4394. PMID: 37853695.
      View in: PubMed
    2. Mu?oz-Escudero D, Breazeale SD, Lee M, Guan Z, Raetz CRH, Sousa MC. Structure and Function of ArnD. A Deformylase Essential for Lipid A Modification with 4-Amino-4-deoxy-l-arabinose and Polymyxin Resistance. Biochemistry. 2023 10 17; 62(20):2970-2981. PMID: 37782650.
      View in: PubMed
    3. Mitchell ME, Gatzeva-Topalova PZ, Bargmann AD, Sammakia T, Sousa MC. Targeting the Conformational Change in ArnA Dehydrogenase for Selective Inhibition of Polymyxin Resistance. Biochemistry. 2023 07 18; 62(14):2216-2227. PMID: 37410993.
      View in: PubMed
    4. LeBlanc MA, Fink MR, Perkins TT, Sousa MC. Type III secretion system effector proteins are mechanically labile. Proc Natl Acad Sci U S A. 2021 03 23; 118(12). PMID: 33723050.
      View in: PubMed
    5. Hjortness MK, Riccardi L, Hongdusit A, Ruppe S, Zhao M, Kim EY, Zwart PH, Sankaran B, Arthanari H, Sousa MC, De Vivo M, Fox JM. Correction to "Abietane-Type Diterpenoids Inhibit Protein Tyrosine Phosphatases by Stabilizing an Inactive Enzyme Conformation". Biochemistry. 2019 06 18; 58(24):2797. PMID: 31181901.
      View in: PubMed
    6. Hjortness MK, Riccardi L, Hongdusit A, Ruppe S, Zhao M, Kim EY, Zwart PH, Sankaran B, Arthanari H, Sousa MC, De Vivo M, Fox JM. Abietane-Type Diterpenoids Inhibit Protein Tyrosine Phosphatases by Stabilizing an Inactive Enzyme Conformation. Biochemistry. 2018 10 09; 57(40):5886-5896. PMID: 30169954.
      View in: PubMed
    7. Walder R, LeBlanc MA, Van Patten WJ, Edwards DT, Greenberg JA, Adhikari A, Okoniewski SR, Sullan RMA, Rabuka D, Sousa MC, Perkins TT. Rapid Characterization of a Mechanically Labile a-Helical Protein Enabled by Efficient Site-Specific Bioconjugation. J Am Chem Soc. 2017 07 26; 139(29):9867-9875. PMID: 28677396.
      View in: PubMed
    8. Doerner PA, Sousa MC. Extreme Dynamics in the BamA ?-Barrel Seam. Biochemistry. 2017 06 20; 56(24):3142-3149. PMID: 28569500.
      View in: PubMed
    9. Warner LR, Gatzeva-Topalova PZ, Doerner PA, Pardi A, Sousa MC. Flexibility in the Periplasmic Domain of BamA Is Important for Function. Structure. 2017 01 03; 25(1):94-106. PMID: 27989620.
      View in: PubMed
    10. Fleming PJ, Patel DS, Wu EL, Qi Y, Yeom MS, Sousa MC, Fleming KG, Im W. BamA POTRA Domain Interacts with a Native Lipid Membrane Surface. Biophys J. 2016 Jun 21; 110(12):2698-2709. PMID: 27332128.
      View in: PubMed
    11. Bergal HT, Hopkins AH, Metzner SI, Sousa MC. The Structure of a BamA-BamD Fusion Illuminates the Architecture of the ?-Barrel Assembly Machine Core. Structure. 2016 Feb 02; 24(2):243-51. PMID: 26749448.
      View in: PubMed
    12. Nordwald EM, Plaks JG, Snell JR, Sousa MC, Kaar JL. Crystallographic Investigation of Imidazolium Ionic Liquid Effects on Enzyme Structure. Chembiochem. 2015 Nov; 16(17):2456-9. PMID: 26388426.
      View in: PubMed
    13. Edwards DT, Faulk JK, Sanders AW, Bull MS, Walder R, LeBlanc MA, Sousa MC, Perkins TT. Optimizing 1-?s-Resolution Single-Molecule Force Spectroscopy on a Commercial Atomic Force Microscope. Nano Lett. 2015 Oct 14; 15(10):7091-8. PMID: 26421945.
      View in: PubMed
    14. Doerner PA, Sousa MC. Small Angle X-ray Scattering (SAXS) Characterization of the POTRA Domains of BamA. Methods Mol Biol. 2015; 1329:149-55. PMID: 26427682.
      View in: PubMed
    15. Jansen KB, Baker SL, Sousa MC. Crystal structure of BamB bound to a periplasmic domain fragment of BamA, the central component of the ?-barrel assembly machine. J Biol Chem. 2015 Jan 23; 290(4):2126-36. PMID: 25468906.
      View in: PubMed
    16. Turco MM, Sousa MC. The structure and specificity of the type III secretion system effector NleC suggest a DNA mimicry mechanism of substrate recognition. Biochemistry. 2014 Aug 12; 53(31):5131-9. PMID: 25040221.
      View in: PubMed
    17. Lee M, Sousa MC. Structural basis for substrate specificity in ArnB. A key enzyme in the polymyxin resistance pathway of Gram-negative bacteria. Biochemistry. 2014 Feb 04; 53(4):796-805. PMID: 24460375.
      View in: PubMed
    18. Templeton PD, Litman ES, Metzner SI, Ahn NG, Sousa MC. Structure of mediator of RhoA-dependent invasion (MRDI) explains its dual function as a metabolic enzyme and a mediator of cell invasion. Biochemistry. 2013 Aug 20; 52(33):5675-84. PMID: 23859498.
      View in: PubMed
    19. Jansen KB, Baker SL, Sousa MC. Crystal structure of BamB from Pseudomonas aeruginosa and functional evaluation of its conserved structural features. PLoS One. 2012; 7(11):e49749. PMID: 23189157.
      View in: PubMed
    20. Warner LR, Varga K, Lange OF, Baker SL, Baker D, Sousa MC, Pardi A. Structure of the BamC two-domain protein obtained by Rosetta with a limited NMR data set. J Mol Biol. 2011 Aug 05; 411(1):83-95. PMID: 21624375.
      View in: PubMed
    21. Sandoval CM, Baker SL, Jansen K, Metzner SI, Sousa MC. Crystal structure of BamD: an essential component of the ?-Barrel assembly machinery of gram-negative bacteria. J Mol Biol. 2011 Jun 10; 409(3):348-57. PMID: 21463635.
      View in: PubMed
    22. Gatzeva-Topalova PZ, Warner LR, Pardi A, Sousa MC. Structure and flexibility of the complete periplasmic domain of BamA: the protein insertion machine of the outer membrane. Structure. 2010 Nov 10; 18(11):1492-501. PMID: 21070948.
      View in: PubMed
    23. Orban T, Bereta G, Miyagi M, Wang B, Chance MR, Sousa MC, Palczewski K. Conformational changes in guanylate cyclase-activating protein 1 induced by Ca2+ and N-terminal fatty acid acylation. Structure. 2010 Jan 13; 18(1):116-26. PMID: 20152158.
      View in: PubMed
    24. Kabuyama Y, Litman ES, Templeton PD, Metzner SI, Witze ES, Argast GM, Langer SJ, Polvinen K, Shellman Y, Chan D, Shabb JB, Fitzpatrick JE, Resing KA, Sousa MC, Ahn NG. A mediator of Rho-dependent invasion moonlights as a methionine salvage enzyme. Mol Cell Proteomics. 2009 Oct; 8(10):2308-20. PMID: 19620624.
      View in: PubMed
    25. Walton TA, Sandoval CM, Fowler CA, Pardi A, Sousa MC. The cavity-chaperone Skp protects its substrate from aggregation but allows independent folding of substrate domains. Proc Natl Acad Sci U S A. 2009 Feb 10; 106(6):1772-7. PMID: 19181847.
      View in: PubMed
    26. Gatzeva-Topalova PZ, Walton TA, Sousa MC. Crystal structure of YaeT: conformational flexibility and substrate recognition. Structure. 2008 Dec 10; 16(12):1873-81. PMID: 19081063.
      View in: PubMed
    27. Stephen R, Filipek S, Palczewski K, Sousa MC. Ca2+ -dependent regulation of phototransduction. Photochem Photobiol. 2008 Jul-Aug; 84(4):903-10. PMID: 18346093.
      View in: PubMed
    28. Stephen R, Bereta G, Golczak M, Palczewski K, Sousa MC. Stabilizing function for myristoyl group revealed by the crystal structure of a neuronal calcium sensor, guanylate cyclase-activating protein 1. Structure. 2007 Nov; 15(11):1392-402. PMID: 17997965.
      View in: PubMed
    29. Stephen R, Palczewski K, Sousa MC. The crystal structure of GCAP3 suggests molecular mechanism of GCAP-linked cone dystrophies. J Mol Biol. 2006 Jun 02; 359(2):266-75. PMID: 16626734.
      View in: PubMed
    30. Gatzeva-Topalova PZ, May AP, Sousa MC. Structure and mechanism of ArnA: conformational change implies ordered dehydrogenase mechanism in key enzyme for polymyxin resistance. Structure. 2005 Jun; 13(6):929-42. PMID: 15939024.
      View in: PubMed
    31. Gatzeva-Topalova PZ, May AP, Sousa MC. Crystal structure and mechanism of the Escherichia coli ArnA (PmrI) transformylase domain. An enzyme for lipid A modification with 4-amino-4-deoxy-L-arabinose and polymyxin resistance. Biochemistry. 2005 Apr 12; 44(14):5328-38. PMID: 15807526.
      View in: PubMed
    32. Gatzeva-Topalova PZ, May AP, Sousa MC. Crystal structure of Escherichia coli ArnA (PmrI) decarboxylase domain. A key enzyme for lipid A modification with 4-amino-4-deoxy-L-arabinose and polymyxin resistance. Biochemistry. 2004 Oct 26; 43(42):13370-9. PMID: 15491143.
      View in: PubMed
    33. Walton TA, Sousa MC. Crystal structure of Skp, a prefoldin-like chaperone that protects soluble and membrane proteins from aggregation. Mol Cell. 2004 Aug 13; 15(3):367-74. PMID: 15304217.
      View in: PubMed
    34. Ridge KD, Abdulaev NG, Sousa M, Palczewski K. Phototransduction: crystal clear. Trends Biochem Sci. 2003 Sep; 28(9):479-87. PMID: 13678959.
      View in: PubMed
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