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Deborah S Wuttke

TitleProfessor
InstitutionUniversity of Colorado Boulder
DepartmentChemistry & Biochemistry
Phone303/492-4576
    Other Positions
    TitleAssoc Chair
    InstitutionUniversity of Colorado Boulder
    DepartmentChemistry & Biochemistry


    Collapse Research 
    Collapse research activities and funding
    R01GM059414     (WUTTKE, DEBORAH S.)May 1, 1999 - May 31, 2016
    NIH/NIGMS
    Single Stranded DNA Recognition in Telomeres
    Role: Principal Investigator
    P41GM068928     (WUTTKE, DEBORAH S.)Jul 15, 2003 - Apr 30, 2012
    NIH/NIGMS
    Purchase of 900 MHz NMR Spectrometer
    Role: Principal Investigator
    R21NS059370     (WUTTKE, DEBORAH S.)Mar 5, 2007 - Feb 28, 2009
    NIH/NINDS
    Development of a HTS Assay Targeting End Protection of Telomeres
    Role: Principal Investigator
    S10RR028036     (WUTTKE, DEBORAH S.)Apr 15, 2010 - Apr 14, 2011
    NIH/NCRR
    Purchase of a CD Spectropolarimeter
    Role: Principal Investigator
    R01GM120347     (WUTTKE, DEBORAH S.)Sep 22, 2016 - Jun 30, 2020
    NIH/NIGMS
    lncRNAs as Organizers of and Bridges Between Proteins and DNA
    Role: Principal Investigator

    Collapse Bibliographic 
    Collapse selected publications
    Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Faculty can login to make corrections and additions.
    List All   |   Timeline
    1. McKercher MA, Guan X, Tan Z, Wuttke DS. Multimodal Recognition of Diverse Peptides by the C-Terminal SH2 Domain of Phospholipase C-?1 Protein. Biochemistry. 2017 Apr 25; 56(16):2225-2237. PMID: 28376302.
      View in: PubMed
    2. McKercher MA, Wuttke DS. Calorimetric Measurement of SH2 Domain Ligand Affinities. Methods Mol Biol. 2017; 1555:291-305. PMID: 28092039.
      View in: PubMed
    3. McKercher MA, Wuttke DS. NMR Chemical Shift Mapping of SH2 Peptide Interactions. Methods Mol Biol. 2017; 1555:269-290. PMID: 28092038.
      View in: PubMed
    4. Lloyd NR, Dickey TH, Hom RA, Wuttke DS. Tying up the Ends: Plasticity in the Recognition of Single-Stranded DNA at Telomeres. Biochemistry. 2016 Sep 27; 55(38):5326-40. PMID: 27575340.
      View in: PubMed
    5. Pinzaru AM, Hom RA, Beal A, Phillips AF, Ni E, Cardozo T, Nair N, Choi J, Wuttke DS, Sfeir A, Denchi EL. Telomere Replication Stress Induced by POT1 Inactivation Accelerates Tumorigenesis. Cell Rep. 2016 Jun 07; 15(10):2170-84. PMID: 27239034.
      View in: PubMed
    6. Lloyd NR, Wuttke DS. Less is more: structures of difficult targets with minimal constraints. Structure. 2014 Sep 02; 22(9):1223-4. PMID: 25185825; PMCID: PMC4260533.
      View in: PubMed, PubMed Central
    7. Dickey TH, Wuttke DS. The telomeric protein Pot1 from Schizosaccharomyces pombe binds ssDNA in two modes with differing 3' end availability. Nucleic Acids Res. 2014 Sep; 42(15):9656-65. PMID: 25074378; PMCID: PMC4150783.
      View in: PubMed, PubMed Central
    8. Rao T, Lubin JW, Armstrong GS, Tucey TM, Lundblad V, Wuttke DS. Structure of Est3 reveals a bimodal surface with differential roles in telomere replication. Proc Natl Acad Sci U S A. 2014 Jan 07; 111(1):214-8. PMID: 24344315; PMCID: PMC3890887.
      View in: PubMed, PubMed Central
    9. Lewis KA, Pfaff DA, Earley JN, Altschuler SE, Wuttke DS. The tenacious recognition of yeast telomere sequence by Cdc13 is fully exerted by a single OB-fold domain. Nucleic Acids Res. 2014 Jan; 42(1):475-84. PMID: 24057216; PMCID: PMC3874162.
      View in: PubMed, PubMed Central
    10. Dickey TH, Altschuler SE, Wuttke DS. Single-stranded DNA-binding proteins: multiple domains for multiple functions. Structure. 2013 Jul 02; 21(7):1074-84. PMID: 23823326; PMCID: PMC3816740.
      View in: PubMed, PubMed Central
    11. Lubin JW, Rao T, Mandell EK, Wuttke DS, Lundblad V. Dissecting protein function: an efficient protocol for identifying separation-of-function mutations that encode structurally stable proteins. Genetics. 2013 Mar; 193(3):715-25. PMID: 23307900; PMCID: PMC3583993.
      View in: PubMed, PubMed Central
    12. Altschuler SE, Lewis KA, Wuttke DS. Practical strategies for the evaluation of high-affinity protein/nucleic acid interactions. J Nucleic Acids Investig. 2013 Jan 01; 4(1):19-28. PMID: 25197549.
      View in: PubMed
    13. Dickey TH, McKercher MA, Wuttke DS. Nonspecific recognition is achieved in Pot1pC through the use of multiple binding modes. Structure. 2013 Jan 08; 21(1):121-32. PMID: 23201273; PMCID: PMC3545015.
      View in: PubMed, PubMed Central
    14. Altschuler SE, Croy JE, Wuttke DS. A small molecule inhibitor of Pot1 binding to telomeric DNA. Biochemistry. 2012 Oct 09; 51(40):7833-45. PMID: 22978652; PMCID: PMC3514547.
      View in: PubMed, PubMed Central
    15. Lewis KA, Wuttke DS. Telomerase and telomere-associated proteins: structural insights into mechanism and evolution. Structure. 2012 Jan 11; 20(1):28-39. PMID: 22244753; PMCID: PMC4180718.
      View in: PubMed, PubMed Central
    16. Braun LJ, Eldridge AM, Cummiskey J, Arthur KK, Wuttke DS. The role of adjuvant in mediating antigen structure and stability. J Pharm Sci. 2012 Apr; 101(4):1391-9. PMID: 22213631.
      View in: PubMed
    17. Altschuler SE, Dickey TH, Wuttke DS. Schizosaccharomyces pombe protection of telomeres 1 utilizes alternate binding modes to accommodate different telomeric sequences. Biochemistry. 2011 Sep 06; 50(35):7503-13. PMID: 21815629; PMCID: PMC3184176.
      View in: PubMed, PubMed Central
    18. Mandell EK, Gelinas AD, Wuttke DS, Lundblad V. Sequence-specific binding to telomeric DNA is not a conserved property of the Cdc13 DNA binding domain. Biochemistry. 2011 Jul 26; 50(29):6289-91. PMID: 21668015.
      View in: PubMed
    19. Alford JR, Fowler AC, Wuttke DS, Kerwin BA, Latypov RF, Carpenter JF, Randolph TW. Effect of benzyl alcohol on recombinant human interleukin-1 receptor antagonist structure and hydrogen-deuterium exchange. J Pharm Sci. 2011 Oct; 100(10):4215-24. PMID: 21557223.
      View in: PubMed
    20. Lee J, Mandell EK, Rao T, Wuttke DS, Lundblad V. Investigating the role of the Est3 protein in yeast telomere replication. Nucleic Acids Res. 2010 Apr; 38(7):2279-90. PMID: 20047960; PMCID: PMC2853109.
      View in: PubMed, PubMed Central
    21. Gelinas AD, Paschini M, Reyes FE, Héroux A, Batey RT, Lundblad V, Wuttke DS. Telomere capping proteins are structurally related to RPA with an additional telomere-specific domain. Proc Natl Acad Sci U S A. 2009 Nov 17; 106(46):19298-303. PMID: 19884503; PMCID: PMC2780795.
      View in: PubMed, PubMed Central
    22. Croy JE, Altschuler SE, Grimm NE, Wuttke DS. Nonadditivity in the recognition of single-stranded DNA by the schizosaccharomyces pombe protection of telomeres 1 DNA-binding domain, Pot1-DBD. Biochemistry. 2009 Jul 28; 48(29):6864-75. PMID: 19518131; PMCID: PMC2756145.
      View in: PubMed, PubMed Central
    23. Croy JE, Wuttke DS. Insights into the dynamics of specific telomeric single-stranded DNA recognition by Pot1pN. J Mol Biol. 2009 Apr 10; 387(4):935-48. PMID: 19232358; PMCID: PMC4213129.
      View in: PubMed, PubMed Central
    24. Zappulla DC, Roberts JN, Goodrich KJ, Cech TR, Wuttke DS. Inhibition of yeast telomerase action by the telomeric ssDNA-binding protein, Cdc13p. Nucleic Acids Res. 2009 Feb; 37(2):354-67. PMID: 19043074; PMCID: PMC2632905.
      View in: PubMed, PubMed Central
    25. Alford JR, Kwok SC, Roberts JN, Wuttke DS, Kendrick BS, Carpenter JF, Randolph TW. High concentration formulations of recombinant human interleukin-1 receptor antagonist: I. Physical characterization. J Pharm Sci. 2008 Aug; 97(8):3035-50. PMID: 17973297.
      View in: PubMed
    26. Croy JE, Fast JL, Grimm NE, Wuttke DS. Deciphering the mechanism of thermodynamic accommodation of telomeric oligonucleotide sequences by the Schizosaccharomyces pombe protection of telomeres 1 (Pot1pN) protein. Biochemistry. 2008 Apr 15; 47(15):4345-58. PMID: 18355038; PMCID: PMC3987967.
      View in: PubMed, PubMed Central
    27. Eldridge AM, Wuttke DS. Probing the mechanism of recognition of ssDNA by the Cdc13-DBD. Nucleic Acids Res. 2008 Mar; 36(5):1624-33. PMID: 18250086; PMCID: PMC2275150.
      View in: PubMed, PubMed Central
    28. Theobald DL, Wuttke DS. Accurate structural correlations from maximum likelihood superpositions. PLoS Comput Biol. 2008 Feb; 4(2):e43. PMID: 18282091; PMCID: PMC2242818.
      View in: PubMed, PubMed Central
    29. Strauss DM, Wuttke DS. Characterization of protein-protein interactions critical for poliovirus replication: analysis of 3AB and VPg binding to the RNA-dependent RNA polymerase. J Virol. 2007 Jun; 81(12):6369-78. PMID: 17409142; PMCID: PMC1900118.
      View in: PubMed, PubMed Central
    30. Theobald DL, Wuttke DS. Empirical Bayes hierarchical models for regularizing maximum likelihood estimation in the matrix Gaussian Procrustes problem. Proc Natl Acad Sci U S A. 2006 Dec 05; 103(49):18521-7. PMID: 17130458; PMCID: PMC1664551.
      View in: PubMed, PubMed Central
    31. Croy JE, Wuttke DS. Themes in ssDNA recognition by telomere-end protection proteins. Trends Biochem Sci. 2006 Sep; 31(9):516-25. PMID: 16890443.
      View in: PubMed
    32. Croy JE, Podell ER, Wuttke DS. A new model for Schizosaccharomyces pombe telomere recognition: the telomeric single-stranded DNA-binding activity of Pot11-389. J Mol Biol. 2006 Aug 04; 361(1):80-93. PMID: 16842820.
      View in: PubMed
    33. Theobald DL, Wuttke DS. THESEUS: maximum likelihood superpositioning and analysis of macromolecular structures. Bioinformatics. 2006 Sep 01; 22(17):2171-2. PMID: 16777907; PMCID: PMC2584349.
      View in: PubMed, PubMed Central
    34. Eldridge AM, Halsey WA, Wuttke DS. Identification of the determinants for the specific recognition of single-strand telomeric DNA by Cdc13. Biochemistry. 2006 Jan 24; 45(3):871-9. PMID: 16411763; PMCID: PMC3514546.
      View in: PubMed, PubMed Central
    35. Theobald DL, Wuttke DS. Divergent evolution within protein superfolds inferred from profile-based phylogenetics. J Mol Biol. 2005 Dec 02; 354(3):722-37. PMID: 16266719; PMCID: PMC1769326.
      View in: PubMed, PubMed Central
    36. Jacobs SA, Podell ER, Wuttke DS, Cech TR. Soluble domains of telomerase reverse transcriptase identified by high-throughput screening. Protein Sci. 2005 Aug; 14(8):2051-8. PMID: 16046627; PMCID: PMC2279316.
      View in: PubMed, PubMed Central
    37. Theobald DL, Wuttke DS. Prediction of multiple tandem OB-fold domains in telomere end-binding proteins Pot1 and Cdc13. Structure. 2004 Oct; 12(10):1877-9. PMID: 15458635.
      View in: PubMed
    38. Mitton-Fry RM, Anderson EM, Theobald DL, Glustrom LW, Wuttke DS. Structural basis for telomeric single-stranded DNA recognition by yeast Cdc13. J Mol Biol. 2004 Apr 23; 338(2):241-55. PMID: 15066429.
      View in: PubMed
    39. Wuttke DS. Targeting the end; the structure of the Ku80 C-terminal domain. Structure. 2004 Mar; 12(3):355-6. PMID: 15016349.
      View in: PubMed
    40. Theobald DL, Cervantes RB, Lundblad V, Wuttke DS. Homology among telomeric end-protection proteins. Structure. 2003 Sep; 11(9):1049-50. PMID: 12962623.
      View in: PubMed
    41. Strauss DM, Glustrom LW, Wuttke DS. Towards an understanding of the poliovirus replication complex: the solution structure of the soluble domain of the poliovirus 3A protein. J Mol Biol. 2003 Jul 04; 330(2):225-34. PMID: 12823963.
      View in: PubMed
    42. Anderson EM, Halsey WA, Wuttke DS. Site-directed mutagenesis reveals the thermodynamic requirements for single-stranded DNA recognition by the telomere-binding protein Cdc13. Biochemistry. 2003 Apr 08; 42(13):3751-8. PMID: 12667066.
      View in: PubMed
    43. Theobald DL, Mitton-Fry RM, Wuttke DS. Nucleic acid recognition by OB-fold proteins. Annu Rev Biophys Biomol Struct. 2003; 32:115-33. PMID: 12598368; PMCID: PMC1564333.
      View in: PubMed, PubMed Central
    44. Ojennus DD, Lehto SE, Wuttke DS. Electrostatic interactions in the reconstitution of an SH2 domain from constituent peptide fragments. Protein Sci. 2003 Jan; 12(1):44-55. PMID: 12493827; PMCID: PMC2312404.
      View in: PubMed, PubMed Central
    45. Anderson EM, Halsey WA, Wuttke DS. Delineation of the high-affinity single-stranded telomeric DNA-binding domain of Saccharomyces cerevisiae Cdc13. Nucleic Acids Res. 2002 Oct 01; 30(19):4305-13. PMID: 12364610; PMCID: PMC140553.
      View in: PubMed, PubMed Central
    46. Glustrom LW, Mitton-Fry RM, Wuttke DS. Re: 1,1-Dichloro-2,2-bis-(p-chlorophenyl)ethylene and polychlorinated biphenyls and breast cancer: combined analysis of five U.S. studies. J Natl Cancer Inst. 2002 Sep 04; 94(17):1337-8; author reply 1338-9. PMID: 12208902.
      View in: PubMed
    47. Mitton-Fry RM, Anderson EM, Hughes TR, Lundblad V, Wuttke DS. Conserved structure for single-stranded telomeric DNA recognition. Science. 2002 Apr 05; 296(5565):145-7. PMID: 11935027.
      View in: PubMed
    48. Mitton-Fry RM, Wuttke DS. 1H, 13C and 15N resonance assignments of the DNA-binding domain of the essential protein Cdc13 complexed with single-stranded telomeric DNA. J Biomol NMR. 2002 Apr; 22(4):379-80. PMID: 12018489.
      View in: PubMed
    49. Ojennus DD, Fleissner MR, Wuttke DS. Reconstitution of a native-like SH2 domain from disordered peptide fragments examined by multidimensional heteronuclear NMR. Protein Sci. 2001 Nov; 10(11):2162-75. PMID: 11604523; PMCID: PMC2374061.
      View in: PubMed, PubMed Central
    50. Dahlke Ojennus D, Mitton-Fry RM, Wuttke DS. Induced alignment and measurement of dipolar couplings of an SH2 domain through direct binding with filamentous phage. J Biomol NMR. 1999 Jun; 14(2):175-9. PMID: 21080256.
      View in: PubMed
    51. Foster MP, Wuttke DS, Clemens KR, Jahnke W, Radhakrishnan I, Tennant L, Reymond M, Chung J, Wright PE. Chemical shift as a probe of molecular interfaces: NMR studies of DNA binding by the three amino-terminal zinc finger domains from transcription factor IIIA. J Biomol NMR. 1998 Jul; 12(1):51-71. PMID: 9729788.
      View in: PubMed
    52. Wuttke DS, Foster MP, Case DA, Gottesfeld JM, Wright PE. Solution structure of the first three zinc fingers of TFIIIA bound to the cognate DNA sequence: determinants of affinity and sequence specificity. J Mol Biol. 1997 Oct 17; 273(1):183-206. PMID: 9367756.
      View in: PubMed
    53. Foster MP, Wuttke DS, Radhakrishnan I, Case DA, Gottesfeld JM, Wright PE. Domain packing and dynamics in the DNA complex of the N-terminal zinc fingers of TFIIIA. Nat Struct Biol. 1997 Aug; 4(8):605-8. PMID: 9253405.
      View in: PubMed
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