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Joseph Falke

TitleProfessor
InstitutionUniversity of Colorado Boulder
DepartmentBiochemistry
Phone303/492-3503

    Collapse Research 
    Collapse research activities and funding
    S10RR024539     (FALKE, JOSEPH J)Apr 1, 2008 - Mar 31, 2010
    NIH
    Acquisition of a Continuous Wave X-band EPR Spectrometer
    Role: Principal Investigator
    R13AI069899     (FALKE, JOSEPH J)Feb 15, 2006 - Jan 31, 2007
    NIH
    Bacterial invasion &phagocytosis
    Role: Principal Investigator
    R13GM067188     (FALKE, JOSEPH J)Sep 10, 2002 - Aug 31, 2003
    NIH
    Membrane Proteins - Structure and Mechanism
    Role: Principal Investigator
    T32GM065103     (FALKE, JOSEPH J)Jul 1, 2002 - Jun 30, 2022
    NIH
    Interdisciplinary Predoctoral Training in Molecular Biophysics
    Role: Principal Investigator
    R01GM063235     (FALKE, JOSEPH J)Apr 1, 2001 - Feb 28, 2022
    NIH
    Mechanisms of Signaling on Membrane Surfaces
    Role: Principal Investigator
    R13AI041086     (FALKE, JOSEPH J)Mar 1, 1997 - Feb 28, 1998
    NIH
    SCIENTIFIC CONFERENCE--BLAST IV
    Role: Principal Investigator
    R01GM048203     (FALKE, JOSEPH J)Aug 1, 1992 - Mar 31, 2001
    NIH
    MOLECULAR STUDIES OF CALCIUM BINDING AND SIGNALING
    Role: Principal Investigator
    R01GM040731     (FALKE, JOSEPH J.)Jul 1, 1988 - Mar 31, 2014
    NIH
    Activation and Dynamics of Receptors and Kinases
    Role: Principal Investigator
    F32GM010428     (FALKE, JOSEPH J)Oct 1, 1985
    NIH
    TRANSMEMBRANE SIGNALING BY A CELL-SURFACE RECEPTOR
    Role: Principal Investigator

    Collapse Bibliographic 
    Collapse selected publications
    Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Faculty can login to make corrections and additions.
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    1. Gordon MT, Ziemba BP, Falke JJ. PDK1:PKCa heterodimer association-dissociation dynamics in single-molecule diffusion tracks on a target membrane. Biophys J. 2023 06 06; 122(11):2301-2310. PMID: 36733254.
      View in: PubMed
    2. Fleming IR, Hannan JP, Swisher GH, Tesdahl CD, Martyr JG, Cordaro NJ, Erbse AH, Falke JJ. Binding of active Ras and its mutants to the Ras binding domain of PI-3-kinase: A quantitative approach to KD measurements. Anal Biochem. 2023 02 15; 663:115019. PMID: 36526022.
      View in: PubMed
    3. Gordon MT, Ziemba BP, Falke JJ. Single-molecule studies reveal regulatory interactions between master kinases PDK1, AKT1, and PKC. Biophys J. 2021 12 21; 120(24):5657-5673. PMID: 34673053.
      View in: PubMed
    4. Hannan JP, Swisher GH, Martyr JG, Cordaro NJ, Erbse AH, Falke JJ. HPLC method to resolve, identify and quantify guanine nucleotides bound to recombinant ras GTPase. Anal Biochem. 2021 10 15; 631:114338. PMID: 34433016.
      View in: PubMed
    5. Swisher GH, Hannan JP, Cordaro NJ, Erbse AH, Falke JJ. Ras-guanine nucleotide complexes: A UV spectral deconvolution method to analyze protein concentration, nucleotide stoichiometry, and purity. Anal Biochem. 2021 04 01; 618:114066. PMID: 33485819.
      View in: PubMed
    6. Buckles TC, Ohashi Y, Tremel S, McLaughlin SH, Pardon E, Steyaert J, Gordon MT, Williams RL, Falke JJ. The G-Protein Rab5A Activates VPS34 Complex II, a Class III PI3K, by a Dual Regulatory Mechanism. Biophys J. 2020 12 01; 119(11):2205-2218. PMID: 33137306.
      View in: PubMed
    7. Buckles TC, Ziemba BP, Djukovic D, Falke JJ. Rapid exposure of macrophages to drugs resolves four classes of effects on the leading edge sensory pseudopod: Non-perturbing, adaptive, disruptive, and activating. PLoS One. 2020; 15(5):e0233012. PMID: 32469878.
      View in: PubMed
    8. Ziemba BP, Falke JJ. A PKC-MARCKS-PI3K regulatory module links Ca2+ and PIP3 signals at the leading edge of polarized macrophages. PLoS One. 2018; 13(5):e0196678. PMID: 29715315.
      View in: PubMed
    9. Buckles TC, Ziemba BP, Masson GR, Williams RL, Falke JJ. Single-Molecule Study Reveals How Receptor and Ras Synergistically Activate PI3Ka and PIP3 Signaling. Biophys J. 2017 Dec 05; 113(11):2396-2405. PMID: 29211993.
      View in: PubMed
    10. Ziemba BP, Swisher GH, Masson G, Burke JE, Williams RL, Falke JJ. Regulation of a Coupled MARCKS-PI3K Lipid Kinase Circuit by Calmodulin: Single-Molecule Analysis of a Membrane-Bound Signaling Module. Biochemistry. 2016 Nov 22; 55(46):6395-6405. PMID: 27933776.
      View in: PubMed
    11. Ziemba BP, Burke JE, Masson G, Williams RL, Falke JJ. Regulation of PI3K by PKC and MARCKS: Single-Molecule Analysis of a Reconstituted Signaling Pathway. Biophys J. 2016 04 26; 110(8):1811-1825. PMID: 27119641.
      View in: PubMed
    12. Parkinson JS, Hazelbauer GL, Falke JJ. Signaling and sensory adaptation in Escherichia coli chemoreceptors: 2015 update. Trends Microbiol. 2015 May; 23(5):257-66. PMID: 25834953.
      View in: PubMed
    13. Falke JJ, Piasta KN. Architecture and signal transduction mechanism of the bacterial chemosensory array: progress, controversies, and challenges. Curr Opin Struct Biol. 2014 Dec; 29:85-94. PMID: 25460272.
      View in: PubMed
    14. Falke JJ. Piston versus scissors: chemotaxis receptors versus sensor His-kinase receptors in two-component signaling pathways. Structure. 2014 Sep 02; 22(9):1219-1220. PMID: 25185823.
      View in: PubMed
    15. Piasta KN, Falke JJ. Increasing and decreasing the ultrastability of bacterial chemotaxis core signaling complexes by modifying protein-protein contacts. Biochemistry. 2014 Sep 09; 53(35):5592-600. PMID: 25119814.
      View in: PubMed
    16. Li J, Ziemba BP, Falke JJ, Voth GA. Interactions of protein kinase C-a C1A and C1B domains with membranes: a combined computational and experimental study. J Am Chem Soc. 2014 Aug 20; 136(33):11757-66. PMID: 25075641.
      View in: PubMed
    17. Ziemba BP, Li J, Landgraf KE, Knight JD, Voth GA, Falke JJ. Single-molecule studies reveal a hidden key step in the activation mechanism of membrane-bound protein kinase C-a. Biochemistry. 2014 Mar 18; 53(10):1697-713. PMID: 24559055.
      View in: PubMed
    18. Briegel A, Wong ML, Hodges HL, Oikonomou CM, Piasta KN, Harris MJ, Fowler DJ, Thompson LK, Falke JJ, Kiessling LL, Jensen GJ. New insights into bacterial chemoreceptor array structure and assembly from electron cryotomography. Biochemistry. 2014 Mar 18; 53(10):1575-85. PMID: 24580139.
      View in: PubMed
    19. Falke JJ, Ziemba BP. Interplay between phosphoinositide lipids and calcium signals at the leading edge of chemotaxing ameboid cells. Chem Phys Lipids. 2014 Sep; 182:73-9. PMID: 24451847.
      View in: PubMed
    20. Natale AM, Duplantis JL, Piasta KN, Falke JJ. Structure, function, and on-off switching of a core unit contact between CheA kinase and CheW adaptor protein in the bacterial chemosensory array: A disulfide mapping and mutagenesis study. Biochemistry. 2013 Nov 05; 52(44):7753-65. PMID: 24090207.
      View in: PubMed
    21. Ziemba BP, Pilling C, Calleja V, Larijani B, Falke JJ. The PH domain of phosphoinositide-dependent kinase-1 exhibits a novel, phospho-regulated monomer-dimer equilibrium with important implications for kinase domain activation: single-molecule and ensemble studies. Biochemistry. 2013 Jul 16; 52(28):4820-9. PMID: 23745598.
      View in: PubMed
    22. Lai CL, Srivastava A, Pilling C, Chase AR, Falke JJ, Voth GA. Molecular mechanism of membrane binding of the GRP1 PH domain. J Mol Biol. 2013 Sep 09; 425(17):3073-90. PMID: 23747485.
      View in: PubMed
    23. Li X, Fleetwood AD, Bayas C, Bilwes AM, Ortega DR, Falke JJ, Zhulin IB, Crane BR. The 3.2 ? resolution structure of a receptor: CheA:CheW signaling complex defines overlapping binding sites and key residue interactions within bacterial chemosensory arrays. Biochemistry. 2013 Jun 04; 52(22):3852-65. PMID: 23668907.
      View in: PubMed
    24. Piasta KN, Ulliman CJ, Slivka PF, Crane BR, Falke JJ. Defining a key receptor-CheA kinase contact and elucidating its function in the membrane-bound bacterial chemosensory array: a disulfide mapping and TAM-IDS Study. Biochemistry. 2013 Jun 04; 52(22):3866-80. PMID: 23668882.
      View in: PubMed
    25. Ziemba BP, Falke JJ. Lateral diffusion of peripheral membrane proteins on supported lipid bilayers is controlled by the additive frictional drags of (1) bound lipids and (2) protein domains penetrating into the bilayer hydrocarbon core. Chem Phys Lipids. 2013 Jul-Aug; 172-173:67-77. PMID: 23701821.
      View in: PubMed
    26. Slivka PF, Falke JJ. Isolated bacterial chemosensory array possesses quasi- and ultrastable components: functional links between array stability, cooperativity, and order. Biochemistry. 2012 Dec 21; 51(51):10218-28. PMID: 23186266.
      View in: PubMed
    27. Brandt DS, Coffman MD, Falke JJ, Knight JD. Hydrophobic contributions to the membrane docking of synaptotagmin 7 C2A domain: mechanistic contrast between isoforms 1 and 7. Biochemistry. 2012 Oct 02; 51(39):7654-64. PMID: 22966849.
      View in: PubMed
    28. Chen HC, Ziemba BP, Landgraf KE, Corbin JA, Falke JJ. Membrane docking geometry of GRP1 PH domain bound to a target lipid bilayer: an EPR site-directed spin-labeling and relaxation study. PLoS One. 2012; 7(3):e33640. PMID: 22479423.
      View in: PubMed
    29. Ziemba BP, Knight JD, Falke JJ. Assembly of membrane-bound protein complexes: detection and analysis by single molecule diffusion. Biochemistry. 2012 Feb 28; 51(8):1638-47. PMID: 22263647.
      View in: PubMed
    30. Falke JJ. Lipid targeting domain with dual-membrane specificity that expands the diversity of intracellular targeting reactions. Proc Natl Acad Sci U S A. 2012 Feb 07; 109(6):1816-7. PMID: 22308463.
      View in: PubMed
    31. Pilling C, Landgraf KE, Falke JJ. The GRP1 PH domain, like the AKT1 PH domain, possesses a sentry glutamate residue essential for specific targeting to plasma membrane PI(3,4,5)P(3). Biochemistry. 2011 Nov 15; 50(45):9845-56. PMID: 21932773.
      View in: PubMed
    32. Bornhorst BJ, Falke JJ. Reprint of: Purification of Proteins Using Polyhistidine Affinity Tags. Protein Expr Purif. 2011 Sep 03. PMID: 21893196.
      View in: PubMed
    33. Erbse AH, Berlinberg AJ, Cheung CY, Leung WY, Falke JJ. OS-FRET: a new one-sample method for improved FRET measurements. Biochemistry. 2011 Feb 01; 50(4):451-7. PMID: 21110513.
      View in: PubMed
    34. Knight JD, Lerner MG, Marcano-Vel?zquez JG, Pastor RW, Falke JJ. Single molecule diffusion of membrane-bound proteins: window into lipid contacts and bilayer dynamics. Biophys J. 2010 Nov 03; 99(9):2879-87. PMID: 21044585.
      View in: PubMed
    35. Lai CL, Landgraf KE, Voth GA, Falke JJ. Membrane docking geometry and target lipid stoichiometry of membrane-bound PKCa C2 domain: a combined molecular dynamics and experimental study. J Mol Biol. 2010 Sep 17; 402(2):301-10. PMID: 20659476.
      View in: PubMed
    36. Hutchinson MR, Loram LC, Zhang Y, Shridhar M, Rezvani N, Berkelhammer D, Phipps S, Foster PS, Landgraf K, Falke JJ, Rice KC, Maier SF, Yin H, Watkins LR. Evidence that tricyclic small molecules may possess toll-like receptor and myeloid differentiation protein 2 activity. Neuroscience. 2010 Jun 30; 168(2):551-63. PMID: 20381591.
      View in: PubMed
    37. Swain KE, Gonzalez MA, Falke JJ. Engineered socket study of signaling through a four-helix bundle: evidence for a yin-yang mechanism in the kinase control module of the aspartate receptor. Biochemistry. 2009 Oct 06; 48(39):9266-77. PMID: 19705835.
      View in: PubMed
    38. Falke JJ, Erbse AH. The piston rises again. Structure. 2009 Sep 09; 17(9):1149-51. PMID: 19748334.
      View in: PubMed
    39. Hutchinson MR, Zhang Y, Shridhar M, Evans JH, Buchanan MM, Zhao TX, Slivka PF, Coats BD, Rezvani N, Wieseler J, Hughes TS, Landgraf KE, Chan S, Fong S, Phipps S, Falke JJ, Leinwand LA, Maier SF, Yin H, Rice KC, Watkins LR. Evidence that opioids may have toll-like receptor 4 and MD-2 effects. Brain Behav Immun. 2010 Jan; 24(1):83-95. PMID: 19679181.
      View in: PubMed
    40. Erbse AH, Falke JJ. The core signaling proteins of bacterial chemotaxis assemble to form an ultrastable complex. Biochemistry. 2009 Jul 28; 48(29):6975-87. PMID: 19456111.
      View in: PubMed
    41. Gloor SL, Falke JJ. Thermal domain motions of CheA kinase in solution: Disulfide trapping reveals the motional constraints leading to trans-autophosphorylation. Biochemistry. 2009 Apr 28; 48(16):3631-44. PMID: 19256549.
      View in: PubMed
    42. Knight JD, Falke JJ. Single-molecule fluorescence studies of a PH domain: new insights into the membrane docking reaction. Biophys J. 2009 Jan; 96(2):566-82. PMID: 19167305.
      View in: PubMed
    43. Landgraf KE, Pilling C, Falke JJ. Molecular mechanism of an oncogenic mutation that alters membrane targeting: Glu17Lys modifies the PIP lipid specificity of the AKT1 PH domain. Biochemistry. 2008 Nov 25; 47(47):12260-9. PMID: 18954143.
      View in: PubMed
    44. Landgraf KE, Malmberg NJ, Falke JJ. Effect of PIP2 binding on the membrane docking geometry of PKC alpha C2 domain: an EPR site-directed spin-labeling and relaxation study. Biochemistry. 2008 Aug 12; 47(32):8301-16. PMID: 18610985.
      View in: PubMed
    45. Hazelbauer GL, Falke JJ, Parkinson JS. Bacterial chemoreceptors: high-performance signaling in networked arrays. Trends Biochem Sci. 2008 Jan; 33(1):9-19. PMID: 18165013.
      View in: PubMed
    46. Swain KE, Falke JJ. Structure of the conserved HAMP domain in an intact, membrane-bound chemoreceptor: a disulfide mapping study. Biochemistry. 2007 Dec 04; 46(48):13684-95. PMID: 17994770.
      View in: PubMed
    47. Evans JH, Falke JJ. Ca2+ influx is an essential component of the positive-feedback loop that maintains leading-edge structure and activity in macrophages. Proc Natl Acad Sci U S A. 2007 Oct 09; 104(41):16176-81. PMID: 17911247.
      View in: PubMed
    48. Endres RG, Falke JJ, Wingreen NS. Chemotaxis receptor complexes: from signaling to assembly. PLoS Comput Biol. 2007 Jul; 3(7):e150. PMID: 17676982.
      View in: PubMed
    49. Corbin JA, Evans JH, Landgraf KE, Falke JJ. Mechanism of specific membrane targeting by C2 domains: localized pools of target lipids enhance Ca2+ affinity. Biochemistry. 2007 Apr 10; 46(14):4322-36. PMID: 17367165.
      View in: PubMed
    50. Bass RB, Butler SL, Chervitz SA, Gloor SL, Falke JJ. Use of site-directed cysteine and disulfide chemistry to probe protein structure and dynamics: applications to soluble and transmembrane receptors of bacterial chemotaxis. Methods Enzymol. 2007; 423:25-51. PMID: 17609126.
      View in: PubMed
    51. Falke JJ. Membrane Recruitment as a Cancer Mechanism: A Case Study of Akt PH Domain. Cellscience. 2007; 4(2):25-30. PMID: 19079757.
      View in: PubMed
    52. Bass RB, Miller AS, Gloor SL, Falke JJ. The PICM chemical scanning method for identifying domain-domain and protein-protein interfaces: applications to the core signaling complex of E. coli chemotaxis. Methods Enzymol. 2007; 423:3-24. PMID: 17609125.
      View in: PubMed
    53. Jaud S, Tobias DJ, Falke JJ, White SH. Self-induced docking site of a deeply embedded peripheral membrane protein. Biophys J. 2007 Jan 15; 92(2):517-24. PMID: 17071664.
      View in: PubMed
    54. Miller AS, Kohout SC, Gilman KA, Falke JJ. CheA Kinase of bacterial chemotaxis: chemical mapping of four essential docking sites. Biochemistry. 2006 Jul 25; 45(29):8699-711. PMID: 16846213.
      View in: PubMed
    55. Evans JH, Murray D, Leslie CC, Falke JJ. Specific translocation of protein kinase Calpha to the plasma membrane requires both Ca2+ and PIP2 recognition by its C2 domain. Mol Biol Cell. 2006 Jan; 17(1):56-66. PMID: 16236797.
      View in: PubMed
    56. Winston SE, Mehan R, Falke JJ. Evidence that the adaptation region of the aspartate receptor is a dynamic four-helix bundle: cysteine and disulfide scanning studies. Biochemistry. 2005 Sep 27; 44(38):12655-66. PMID: 16171380.
      View in: PubMed
    57. Coleman MD, Bass RB, Mehan RS, Falke JJ. Conserved glycine residues in the cytoplasmic domain of the aspartate receptor play essential roles in kinase coupling and on-off switching. Biochemistry. 2005 May 31; 44(21):7687-95. PMID: 15909983.
      View in: PubMed
    58. Starrett DJ, Falke JJ. Adaptation mechanism of the aspartate receptor: electrostatics of the adaptation subdomain play a key role in modulating kinase activity. Biochemistry. 2005 Feb 08; 44(5):1550-60. PMID: 15683239.
      View in: PubMed
    59. Malmberg NJ, Falke JJ. Use of EPR power saturation to analyze the membrane-docking geometries of peripheral proteins: applications to C2 domains. Annu Rev Biophys Biomol Struct. 2005; 34:71-90. PMID: 15869384.
      View in: PubMed
    60. Corbin JA, Dirkx RA, Falke JJ. GRP1 pleckstrin homology domain: activation parameters and novel search mechanism for rare target lipid. Biochemistry. 2004 Dec 28; 43(51):16161-73. PMID: 15610010.
      View in: PubMed
    61. Malmberg NJ, Varma S, Jakobsson E, Falke JJ. Ca2+ activation of the cPLA2 C2 domain: ordered binding of two Ca2+ ions with positive cooperativity. Biochemistry. 2004 Dec 28; 43(51):16320-8. PMID: 15610026.
      View in: PubMed
    62. Miller AS, Falke JJ. Side chains at the membrane-water interface modulate the signaling state of a transmembrane receptor. Biochemistry. 2004 Feb 24; 43(7):1763-70. PMID: 14967017.
      View in: PubMed
    63. Miller AF, Falke JJ. Chemotaxis receptors and signaling. Adv Protein Chem. 2004; 68:393-444. PMID: 15500867.
      View in: PubMed
    64. Malmberg NJ, Van Buskirk DR, Falke JJ. Membrane-docking loops of the cPLA2 C2 domain: detailed structural analysis of the protein-membrane interface via site-directed spin-labeling. Biochemistry. 2003 Nov 18; 42(45):13227-40. PMID: 14609334.
      View in: PubMed
    65. Mehan RS, White NC, Falke JJ. Mapping out regions on the surface of the aspartate receptor that are essential for kinase activation. Biochemistry. 2003 Mar 18; 42(10):2952-9. PMID: 12627961.
      View in: PubMed
    66. Bornhorst JA, Falke JJ. Quantitative analysis of aspartate receptor signaling complex reveals that the homogeneous two-state model is inadequate: development of a heterogeneous two-state model. J Mol Biol. 2003 Mar 07; 326(5):1597-614. PMID: 12595268.
      View in: PubMed
    67. Kohout SC, Corbal?n-Garc?a S, G?mez-Fern?ndez JC, Falke JJ. C2 domain of protein kinase C alpha: elucidation of the membrane docking surface by site-directed fluorescence and spin labeling. Biochemistry. 2003 Feb 11; 42(5):1254-65. PMID: 12564928.
      View in: PubMed
    68. Kohout SC, Corbal?n-Garc?a S, Torrecillas A, Gom?z-Fernand?z JC, Falke JJ. C2 domains of protein kinase C isoforms alpha, beta, and gamma: activation parameters and calcium stoichiometries of the membrane-bound state. Biochemistry. 2002 Sep 24; 41(38):11411-24. PMID: 12234184.
      View in: PubMed
    69. Frazier AA, Wisner MA, Malmberg NJ, Victor KG, Fanucci GE, Nalefski EA, Falke JJ, Cafiso DS. Membrane orientation and position of the C2 domain from cPLA2 by site-directed spin labeling. Biochemistry. 2002 May 21; 41(20):6282-92. PMID: 12009889.
      View in: PubMed
    70. Falke JJ. Cooperativity between bacterial chemotaxis receptors. Proc Natl Acad Sci U S A. 2002 May 14; 99(10):6530-2. PMID: 12011417.
      View in: PubMed
    71. Falke JJ. Enzymology. A moving story. Science. 2002 Feb 22; 295(5559):1480-1. PMID: 11859184.
      View in: PubMed
    72. Nalefski EA, Falke JJ. Cation charge and size selectivity of the C2 domain of cytosolic phospholipase A(2). Biochemistry. 2002 Jan 29; 41(4):1109-22. PMID: 11802709.
      View in: PubMed
    73. Bornhorst JA, Falke JJ. Evidence that both ligand binding and covalent adaptation drive a two-state equilibrium in the aspartate receptor signaling complex. J Gen Physiol. 2001 Dec; 118(6):693-710. PMID: 11723162.
      View in: PubMed
    74. Falke JJ, Hazelbauer GL. Transmembrane signaling in bacterial chemoreceptors. Trends Biochem Sci. 2001 Apr; 26(4):257-65. PMID: 11295559.
      View in: PubMed
    75. Nalefski EA, Wisner MA, Chen JZ, Sprang SR, Fukuda M, Mikoshiba K, Falke JJ. C2 domains from different Ca2+ signaling pathways display functional and mechanistic diversity. Biochemistry. 2001 Mar 13; 40(10):3089-100. PMID: 11258923.
      View in: PubMed
    76. Bornhorst JA, Falke JJ. Attractant regulation of the aspartate receptor-kinase complex: limited cooperative interactions between receptors and effects of the receptor modification state. Biochemistry. 2000 Aug 08; 39(31):9486-93. PMID: 10924144.
      View in: PubMed
    77. Falke JJ, Kim SH. Structure of a conserved receptor domain that regulates kinase activity: the cytoplasmic domain of bacterial taxis receptors. Curr Opin Struct Biol. 2000 Aug; 10(4):462-9. PMID: 10981636.
      View in: PubMed
    78. Bornhorst JA, Falke JJ. Purification of proteins using polyhistidine affinity tags. Methods Enzymol. 2000; 326:245-54. PMID: 11036646.
      View in: PubMed
    79. Bass RB, Coleman MD, Falke JJ. Signaling domain of the aspartate receptor is a helical hairpin with a localized kinase docking surface: cysteine and disulfide scanning studies. Biochemistry. 1999 Jul 20; 38(29):9317-27. PMID: 10413506.
      View in: PubMed
    80. Bass RB, Falke JJ. The aspartate receptor cytoplasmic domain: in situ chemical analysis of structure, mechanism and dynamics. Structure. 1999 Jul 15; 7(7):829-40. PMID: 10425684.
      View in: PubMed
    81. Trammell MA, Falke JJ. Identification of a site critical for kinase regulation on the central processing unit (CPU) helix of the aspartate receptor. Biochemistry. 1999 Jan 05; 38(1):329-36. PMID: 9890914.
      View in: PubMed
    82. Nalefski EA, Falke JJ. Location of the membrane-docking face on the Ca2+-activated C2 domain of cytosolic phospholipase A2. Biochemistry. 1998 Dec 22; 37(51):17642-50. PMID: 9922129.
      View in: PubMed
    83. Hazard AL, Kohout SC, Stricker NL, Putkey JA, Falke JJ. The kinetic cycle of cardiac troponin C: calcium binding and dissociation at site II trigger slow conformational rearrangements. Protein Sci. 1998 Nov; 7(11):2451-9. PMID: 9828012.
      View in: PubMed
    84. Bass RB, Falke JJ. Detection of a conserved alpha-helix in the kinase-docking region of the aspartate receptor by cysteine and disulfide scanning. J Biol Chem. 1998 Sep 25; 273(39):25006-14. PMID: 9737956.
      View in: PubMed
    85. Butler SL, Falke JJ. Cysteine and disulfide scanning reveals two amphiphilic helices in the linker region of the aspartate chemoreceptor. Biochemistry. 1998 Jul 28; 37(30):10746-56. PMID: 9692965.
      View in: PubMed
    86. Nalefski EA, McDonagh T, Somers W, Seehra J, Falke JJ, Clark JD. Independent folding and ligand specificity of the C2 calcium-dependent lipid binding domain of cytosolic phospholipase A2. J Biol Chem. 1998 Jan 16; 273(3):1365-72. PMID: 9430670.
      View in: PubMed
    87. Danielson MA, Bass RB, Falke JJ. Cysteine and disulfide scanning reveals a regulatory alpha-helix in the cytoplasmic domain of the aspartate receptor. J Biol Chem. 1997 Dec 26; 272(52):32878-88. PMID: 9407066.
      View in: PubMed
    88. Nalefski EA, Slazas MM, Falke JJ. Ca2+-signaling cycle of a membrane-docking C2 domain. Biochemistry. 1997 Oct 07; 36(40):12011-8. PMID: 9340010.
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    89. Drake SK, Zimmer MA, Miller CL, Falke JJ. Optimizing the metal binding parameters of an EF-hand-like calcium chelation loop: coordinating side chains play a more important tuning role than chelation loop flexibility. Biochemistry. 1997 Aug 12; 36(32):9917-26. PMID: 9245425.
      View in: PubMed
    90. Drake SK, Zimmer MA, Kundrot C, Falke JJ. Molecular tuning of an EF-hand-like calcium binding loop. Contributions of the coordinating side chain at loop position 3. J Gen Physiol. 1997 Aug; 110(2):173-84. PMID: 9236210.
      View in: PubMed
    91. Peersen OB, Madsen TS, Falke JJ. Intermolecular tuning of calmodulin by target peptides and proteins: differential effects on Ca2+ binding and implications for kinase activation. Protein Sci. 1997 Apr; 6(4):794-807. PMID: 9098889.
      View in: PubMed
    92. Falke JJ, Bass RB, Butler SL, Chervitz SA, Danielson MA. The two-component signaling pathway of bacterial chemotaxis: a molecular view of signal transduction by receptors, kinases, and adaptation enzymes. Annu Rev Cell Dev Biol. 1997; 13:457-512. PMID: 9442881.
      View in: PubMed
    93. Nalefski EA, Falke JJ. The C2 domain calcium-binding motif: structural and functional diversity. Protein Sci. 1996 Dec; 5(12):2375-90. PMID: 8976547.
      View in: PubMed
    94. Butler SL, Falke JJ. Effects of protein stabilizing agents on thermal backbone motions: a disulfide trapping study. Biochemistry. 1996 Aug 20; 35(33):10595-600. PMID: 8718847.
      View in: PubMed
    95. Drake SK, Lee KL, Falke JJ. Tuning the equilibrium ion affinity and selectivity of the EF-hand calcium binding motif: substitutions at the gateway position. Biochemistry. 1996 May 28; 35(21):6697-705. PMID: 8639620.
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    96. Chervitz SA, Falke JJ. Molecular mechanism of transmembrane signaling by the aspartate receptor: a model. Proc Natl Acad Sci U S A. 1996 Mar 19; 93(6):2545-50. PMID: 8637911.
      View in: PubMed
    97. Drake SK, Falke JJ. Kinetic tuning of the EF-hand calcium binding motif: the gateway residue independently adjusts (i) barrier height and (ii) equilibrium. Biochemistry. 1996 Feb 13; 35(6):1753-60. PMID: 8639655.
      View in: PubMed
    98. Danielson MA, Falke JJ. Use of 19F NMR to probe protein structure and conformational changes. Annu Rev Biophys Biomol Struct. 1996; 25:163-95. PMID: 8800468.
      View in: PubMed
    99. Chervitz SA, Falke JJ. Lock on/off disulfides identify the transmembrane signaling helix of the aspartate receptor. J Biol Chem. 1995 Oct 13; 270(41):24043-53. PMID: 7592603.
      View in: PubMed
    100. Chervitz SA, Lin CM, Falke JJ. Transmembrane signaling by the aspartate receptor: engineered disulfides reveal static regions of the subunit interface. Biochemistry. 1995 Aug 01; 34(30):9722-33. PMID: 7626643.
      View in: PubMed
    101. Falke JJ, Blair DF, Silhavy TJ, Schmitt R. BLAST 1995: international conference on bacterial locomotion and signal transduction. Mol Microbiol. 1995 Jun; 16(6):1037-50. PMID: 8577240.
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    102. Careaga CL, Sutherland J, Sabeti J, Falke JJ. Large amplitude twisting motions of an interdomain hinge: a disulfide trapping study of the galactose-glucose binding protein. Biochemistry. 1995 Mar 07; 34(9):3048-55. PMID: 7893717.
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    103. Falke JJ, Drake SK, Hazard AL, Peersen OB. Molecular tuning of ion binding to calcium signaling proteins. Q Rev Biophys. 1994 Aug; 27(3):219-90. PMID: 7899550.
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    104. Danielson MA, Biemann HP, Koshland DE, Falke JJ. Attractant- and disulfide-induced conformational changes in the ligand binding domain of the chemotaxis aspartate receptor: a 19F NMR study. Biochemistry. 1994 May 24; 33(20):6100-9. PMID: 7910759.
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    105. Renner M, Danielson MA, Falke JJ. Kinetic control of Ca(II) signaling: tuning the ion dissociation rates of EF-hand Ca(II) binding sites. Proc Natl Acad Sci U S A. 1993 Jul 15; 90(14):6493-7. PMID: 8341660.
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    106. Bourret RB, Drake SK, Chervitz SA, Simon MI, Falke JJ. Activation of the phosphosignaling protein CheY. II. Analysis of activated mutants by 19F NMR and protein engineering. J Biol Chem. 1993 Jun 25; 268(18):13089-96. PMID: 8514750.
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    107. Drake SK, Bourret RB, Luck LA, Simon MI, Falke JJ. Activation of the phosphosignaling protein CheY. I. Analysis of the phosphorylated conformation by 19F NMR and protein engineering. J Biol Chem. 1993 Jun 25; 268(18):13081-8. PMID: 8514749.
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    108. Needham JV, Chen TY, Falke JJ. Novel ion specificity of a carboxylate cluster Mg(II) binding site: strong charge selectivity and weak size selectivity. Biochemistry. 1993 Apr 06; 32(13):3363-7. PMID: 8461299.
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    109. Careaga CL, Falke JJ. Thermal motions of surface alpha-helices in the D-galactose chemosensory receptor. Detection by disulfide trapping. J Mol Biol. 1992 Aug 20; 226(4):1219-35. PMID: 1518053.
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    110. Falke JJ, Luck LA, Scherrer J. 19F nuclear magnetic resonance studies of aqueous and transmembrane receptors. Examples from the Escherichia coli chemosensory pathway. Biophys J. 1992 Apr; 62(1):82-6. PMID: 1318106.
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    111. Careaga CL, Falke JJ. Structure and dynamics of Escherichia coli chemosensory receptors. Engineered sulfhydryl studies. Biophys J. 1992 Apr; 62(1):209-16; discussion 217-9. PMID: 1318100.
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    112. Falke JJ, Snyder EE, Thatcher KC, Voertler CS. Quantitating and engineering the ion specificity of an EF-hand-like Ca2+ binding. Biochemistry. 1991 Sep 03; 30(35):8690-7. PMID: 1653605.
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    113. Luck LA, Falke JJ. Open conformation of a substrate-binding cleft: 19F NMR studies of cleft angle in the D-galactose chemosensory receptor. Biochemistry. 1991 Jul 02; 30(26):6484-90. PMID: 1647202.
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    114. Luck LA, Falke JJ. 19F NMR studies of the D-galactose chemosensory receptor. 1. Sugar binding yields a global structural change. Biochemistry. 1991 Apr 30; 30(17):4248-56. PMID: 1850619.
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    115. Luck LA, Falke JJ. 19F NMR studies of the D-galactose chemosensory receptor. 2. Ca(II) binding yields a local structural change. Biochemistry. 1991 Apr 30; 30(17):4257-61. PMID: 1850620.
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    116. Snyder EE, Buoscio BW, Falke JJ. Calcium(II) site specificity: effect of size and charge on metal ion binding to an EF-hand-like site. Biochemistry. 1990 Apr 24; 29(16):3937-43. PMID: 2162201.
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    117. Falke JJ, Dernburg AF, Sternberg DA, Zalkin N, Milligan DL, Koshland DE. Structure of a bacterial sensory receptor. A site-directed sulfhydryl study. J Biol Chem. 1988 Oct 15; 263(29):14850-8. PMID: 3049592.
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    118. Falke JJ, Koshland DE. Global flexibility in a sensory receptor: a site-directed cross-linking approach. Science. 1987 Sep 25; 237(4822):1596-600. PMID: 2820061.
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    119. Falke JJ, Chan SI. Molecular mechanisms of band 3 inhibitors. 1. Transport site inhibitors. Biochemistry. 1986 Dec 02; 25(24):7888-94. PMID: 3801446.
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    120. Falke JJ, Chan SI. Molecular mechanisms of band 3 inhibitors. 3. Translocation inhibitors. Biochemistry. 1986 Dec 02; 25(24):7899-906. PMID: 3801448.
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    121. Falke JJ, Chan SI. Molecular mechanisms of band 3 inhibitors. 2. Channel blockers. Biochemistry. 1986 Dec 02; 25(24):7895-8. PMID: 3801447.
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    122. Wang JF, Falke JJ, Chan SI. A proton NMR study of the mechanism of the erythrocyte glucose transporter. Proc Natl Acad Sci U S A. 1986 May; 83(10):3277-81. PMID: 3458182.
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    123. Falke JJ, Kanes KJ, Chan SI. The minimal structure containing the band 3 anion transport site. A 35Cl NMR study. J Biol Chem. 1985 Oct 25; 260(24):13294-303. PMID: 4055737.
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    124. Falke JJ, Chan SI. Evidence that anion transport by band 3 proceeds via a ping-pong mechanism involving a single transport site. A 35 Cl NMR study. J Biol Chem. 1985 Aug 15; 260(17):9537-44. PMID: 4019484.
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    125. Falke JJ, Kanes KJ, Chan SI. The kinetic equation for the chloride transport cycle of band 3. A 35Cl and 37Cl NMR study. J Biol Chem. 1985 Aug 15; 260(17):9545-51. PMID: 4019485.
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    126. Falke JJ, Pace RJ, Chan SI. Chloride binding to the anion transport binding sites of band 3. A 35Cl NMR study. J Biol Chem. 1984 May 25; 259(10):6472-80. PMID: 6725260.
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    127. Falke JJ, Pace RJ, Chan SI. Direct observation of the transmembrane recruitment of band 3 transport sites by competitive inhibitors. A 35Cl NMR study. J Biol Chem. 1984 May 25; 259(10):6481-91. PMID: 6725261.
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    128. Falke JJ, Chan SI, Steiner M, Oesterhelt D, Towner P, Lanyi JK. Halide binding by the purified halorhodopsin chromoprotein. II. New chloride-binding sites revealed by 35Cl NMR. J Biol Chem. 1984 Feb 25; 259(4):2185-9. PMID: 6698962.
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    129. Falke JJ, Chan SI. Ion Channels within Ion Transport Proteins: Evidence in the Band 3 System. Biophys J. 1984 Jan; 45(1):91-2. PMID: 19431579.
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    130. Falke J, Lazarides E. Staining of viable and nonviable myotubes and of myofibrils by the fluorescent dye merocyanine 540. Differentiation. 1980; 17(3):199-204. PMID: 6161052.
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