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Jeffrey Scott Kieft

InstitutionUniversity of Colorado Denver - Anschutz Medical Campus
DepartmentSOM-BioChem&Molecular Genetics

    Collapse Research 
    Collapse research activities and funding
    R01GM072560     (KIEFT, JEFFREY S)Mar 1, 2005 - Feb 28, 2011
    Structural and biophysical analysis of a viral IRES
    Role: Principal Investigator

    R03AI072187     (KIEFT, JEFFREY S)Jun 1, 2007 - May 31, 2009
    Development of tools for structural genomics of viral RNA
    Role: Principal Investigator

    S10RR024632     (KIEFT, JEFFREY S)Sep 30, 2007 - Sep 29, 2008
    Automated Macromolecular Crystallization System
    Role: Principal Investigator

    R01GM081346     (KIEFT, JEFFREY S)Sep 1, 2008 - May 31, 2017
    Structural basis of protein synthesis by the hepatitis C virus IRES RNA
    Role: Principal Investigator

    R01GM097333     (KIEFT, JEFFREY S)Apr 1, 2012 - Feb 29, 2016
    Manipulation of cellular translation machinery by viral RNAs
    Role: Principal Investigator

    R35GM118070     (KIEFT, JEFFREY S)Aug 1, 2016 - Jul 31, 2021
    Structure, function, and dynamics of viral RNAs and RNA-containing complexes
    Role: Principal Investigator

    R21AI129569     (KIEFT, JEFFREY S)Jan 1, 2017 - Dec 31, 2018
    Understanding pathogenic noncoding RNA production in Zika Virus infection
    Role: Principal Investigator

    R01AI133348     (KIEFT, JEFFREY S)Jun 26, 2017 - May 31, 2022
    Mechanisms of viral RNA maturation by co-opting cellular exonucleases
    Role: Principal Investigator

    Collapse Bibliographic 
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    Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Faculty can login to make corrections and additions.
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    1. Steckelberg AL, Akiyama BM, Costantino DA, Sit TL, Nix JC, Kieft JS. A folded viral noncoding RNA blocks host cell exoribonucleases through a conformationally dynamic RNA structure. Proc Natl Acad Sci U S A. 2018 06 19; 115(25):6404-6409. PMID: 29866852.
      View in: PubMed
    2. MacFadden A, O'Donoghue Z, Silva PAGC, Chapman EG, Olsthoorn RC, Sterken MG, Pijlman GP, Bredenbeek PJ, Kieft JS. Mechanism and structural diversity of exoribonuclease-resistant RNA structures in flaviviral RNAs. Nat Commun. 2018 Jan 09; 9(1):119. PMID: 29317714.
      View in: PubMed
    3. Jaafar ZA, Oguro A, Nakamura Y, Kieft JS. Translation initiation by the hepatitis C virus IRES requires eIF1A and ribosomal complex remodeling. Elife. 2016 12 23; 5. PMID: 28009256.
      View in: PubMed
    4. Akiyama BM, Laurence HM, Massey AR, Costantino DA, Xie X, Yang Y, Shi PY, Nix JC, Beckham JD, Kieft JS. Zika virus produces noncoding RNAs using a multi-pseudoknot structure that confounds a cellular exonuclease. Science. 2016 12 02; 354(6316):1148-1152. PMID: 27934765.
      View in: PubMed
    5. Hussain T, Llácer JL, Wimberly BT, Kieft JS, Ramakrishnan V. Large-Scale Movements of IF3 and tRNA during Bacterial Translation Initiation. Cell. 2016 Sep 22; 167(1):133-144.e13. PMID: 27662086.
      View in: PubMed
    6. Filbin ME, Kieft JS. Linking ? to O: diverse and dynamic RNA-based mechanisms to regulate gene expression by 5'-to-3' communication. F1000Res. 2016; 5. PMID: 27610229; PMCID: PMC4995689.
    7. Hao Y, Kieft JS. Three-way junction conformation dictates self-association of phage packaging RNAs. RNA Biol. 2016 07 02; 13(7):635-45. PMID: 27217219; PMCID: PMC4962800 [Available on 05/24/17].
    8. Akiyama BM, Eiler D, Kieft JS. Structured RNAs that evade or confound exonucleases: function follows form. Curr Opin Struct Biol. 2016 Feb; 36:40-7. PMID: 26797676.
      View in: PubMed
    9. Batey RT, Kieft JS. Soaking Hexammine Cations into RNA Crystals to Obtain Derivatives for Phasing Diffraction Data. Methods Mol Biol. 2016; 1320:219-32. PMID: 26227046.
      View in: PubMed
    10. Ruehle MD, Zhang H, Sheridan RM, Mitra S, Chen Y, Gonzalez RL, Cooperman BS, Kieft JS. A dynamic RNA loop in an IRES affects multiple steps of elongation factor-mediated translation initiation. Elife. 2015 Nov 02; 4. PMID: 26523395; PMCID: PMC4709265.
    11. Ruehle MD, Kieft JS. Molecular prejudice: RNA discrimination against purines allows response to a cellular alarm. Nat Struct Mol Biol. 2015 Oct; 22(10):754-6. PMID: 26439636.
      View in: PubMed
    12. Kieft JS, Rabe JL, Chapman EG. New hypotheses derived from the structure of a flaviviral Xrn1-resistant RNA: Conservation, folding, and host adaptation. RNA Biol. 2015; 12(11):1169-77. PMID: 26399159.
      View in: PubMed
    13. Kieft JS. Experiences gathered and lessons learned from 20 years of RNA structure. RNA. 2015 Apr; 21(4):661-3. PMID: 25780181; PMCID: PMC4371323.
    14. Colussi TM, Costantino DA, Zhu J, Donohue JP, Korostelev AA, Jaafar ZA, Plank TD, Noller HF, Kieft JS. Initiation of translation in bacteria by a structured eukaryotic IRES RNA. Nature. 2015 Mar 05; 519(7541):110-3. PMID: 25652826; PMCID: PMC4352134.
    15. Hao Y, Kieft JS. Diverse self-association properties within a family of phage packaging RNAs. RNA. 2014 Nov; 20(11):1759-74. PMID: 25246655; PMCID: PMC4201828.
    16. Clayton GM, Wang Y, Crawford F, Novikov A, Wimberly BT, Kieft JS, Falta MT, Bowerman NA, Marrack P, Fontenot AP, Dai S, Kappler JW. Structural basis of chronic beryllium disease: linking allergic hypersensitivity and autoimmunity. Cell. 2014 Jul 03; 158(1):132-42. PMID: 24995984; PMCID: PMC4269484.
    17. Colussi TM, Costantino DA, Hammond JA, Ruehle GM, Nix JC, Kieft JS. The structural basis of transfer RNA mimicry and conformational plasticity by a viral RNA. Nature. 2014 Jul 17; 511(7509):366-9. PMID: 24909993; PMCID: PMC4136544.
    18. Chapman EG, Costantino DA, Rabe JL, Moon SL, Wilusz J, Nix JC, Kieft JS. The structural basis of pathogenic subgenomic flavivirus RNA (sfRNA) production. Science. 2014 Apr 18; 344(6181):307-10. PMID: 24744377.
      View in: PubMed
    19. Chapman EG, Moon SL, Wilusz J, Kieft JS. RNA structures that resist degradation by Xrn1 produce a pathogenic Dengue virus RNA. Elife. 2014 Apr 01; 3:e01892. PMID: 24692447.
      View in: PubMed
    20. Plank TD, Whitehurst JT, Cencic R, Pelletier J, Kieft JS. Internal translation initiation from HIV-1 transcripts is conferred by a common RNA structure. Translation (Austin). 2014; 2(1):e27694. PMID: 26779399; PMCID: PMC4705822.
    21. Plank TD, Whitehurst JT, Kieft JS. Cell type specificity and structural determinants of IRES activity from the 5' leaders of different HIV-1 transcripts. Nucleic Acids Res. 2013 Jul; 41(13):6698-714. PMID: 23661682.
      View in: PubMed
    22. Wang H, Ru Y, Sanchez-Carbayo M, Wang X, Kieft JS, Theodorescu D. Translation initiation factor eIF3b expression in human cancer and its role in tumor growth and lung colonization. Clin Cancer Res. 2013 Jun 01; 19(11):2850-60. PMID: 23575475; PMCID: PMC3695228.
    23. Filbin ME, Vollmar BS, Shi D, Gonen T, Kieft JS. HCV IRES manipulates the ribosome to promote the switch from translation initiation to elongation. Nat Struct Mol Biol. 2013 Feb; 20(2):150-8. PMID: 23262488; PMCID: PMC3864654.
    24. Plank TD, Kieft JS. The structures of nonprotein-coding RNAs that drive internal ribosome entry site function. Wiley Interdiscip Rev RNA. 2012 Mar-Apr; 3(2):195-212. PMID: 22215521; PMCID: PMC3973487.
    25. Keel AY, Jha BK, Kieft JS. Structural architecture of an RNA that competitively inhibits RNase L. RNA. 2012 Jan; 18(1):88-99. PMID: 22114318; PMCID: PMC3261747.
    26. Liu W, Jankowska-Anyszka M, Piecyk K, Dickson L, Wallace A, Niedzwiecka A, Stepinski J, Stolarski R, Darzynkiewicz E, Kieft J, Zhao R, Jones DN, Davis RE. Structural basis for nematode eIF4E binding an m(2,2,7)G-Cap and its implications for translation initiation. Nucleic Acids Res. 2011 Nov 01; 39(20):8820-32. PMID: 21965542; PMCID: PMC3203607.
    27. Filbin ME, Kieft JS. HCV IRES domain IIb affects the configuration of coding RNA in the 40S subunit's decoding groove. RNA. 2011 Jul; 17(7):1258-73. PMID: 21606179; PMCID: PMC3138563.
    28. Redzic JS, Armstrong GS, Isern NG, Jones DN, Kieft JS, Eisenmesser EZ. The retinal specific CD147 Ig0 domain: from molecular structure to biological activity. J Mol Biol. 2011 Aug 05; 411(1):68-82. PMID: 21620857; PMCID: PMC3143262.
    29. Vallejos M, Deforges J, Plank TD, Letelier A, Ramdohr P, Abraham CG, Valiente-Echeverría F, Kieft JS, Sargueil B, López-Lastra M. Activity of the human immunodeficiency virus type 1 cell cycle-dependent internal ribosomal entry site is modulated by IRES trans-acting factors. Nucleic Acids Res. 2011 Aug; 39(14):6186-200. PMID: 21482538; PMCID: PMC3152342.
    30. Zhu J, Korostelev A, Costantino DA, Donohue JP, Noller HF, Kieft JS. Crystal structures of complexes containing domains from two viral internal ribosome entry site (IRES) RNAs bound to the 70S ribosome. Proc Natl Acad Sci U S A. 2011 Feb 01; 108(5):1839-44. PMID: 21245352; PMCID: PMC3033271.
    31. Feser J, Truong D, Das C, Carson JJ, Kieft J, Harkness T, Tyler JK. Elevated histone expression promotes life span extension. Mol Cell. 2010 Sep 10; 39(5):724-35. PMID: 20832724; PMCID: PMC3966075.
    32. Kieft JS, Chase E, Costantino DA, Golden BL. Identification and characterization of anion binding sites in RNA. RNA. 2010 Jun; 16(6):1118-23. PMID: 20410239; PMCID: PMC2874163.
    33. Hammond JA, Rambo RP, Kieft JS. Multi-domain packing in the aminoacylatable 3' end of a plant viral RNA. J Mol Biol. 2010 Jun 11; 399(3):450-63. PMID: 20398674; PMCID: PMC3111976.
    34. Constantineau J, Greason E, West M, Filbin M, Kieft JS, Carletti MZ, Christenson LK, Rodriguez A. A synonymous variant in scavenger receptor, class B, type I gene is associated with lower SR-BI protein expression and function. Atherosclerosis. 2010 May; 210(1):177-82. PMID: 20060115; PMCID: PMC3342769.
    35. Keel AY, Easton LE, Lukavsky PJ, Kieft JS. Large-scale native preparation of in vitro transcribed RNA. Methods Enzymol. 2009; 469:3-25. PMID: 20946782.
      View in: PubMed
    36. Pfingsten JS, Castile AE, Kieft JS. Mechanistic role of structurally dynamic regions in Dicistroviridae IGR IRESs. J Mol Biol. 2010 Jan 08; 395(1):205-17. PMID: 19878683; PMCID: PMC2788014.
    37. Liu W, Zhao R, McFarland C, Kieft J, Niedzwiecka A, Jankowska-Anyszka M, Stepinski J, Darzynkiewicz E, Jones DN, Davis RE. Structural insights into parasite eIF4E binding specificity for m7G and m2,2,7G mRNA caps. J Biol Chem. 2009 Nov 06; 284(45):31336-49. PMID: 19710013; PMCID: PMC2781531.
    38. Filbin ME, Kieft JS. Toward a structural understanding of IRES RNA function. Curr Opin Struct Biol. 2009 Jun; 19(3):267-76. PMID: 19362464; PMCID: PMC2757110.
    39. Lee SJ, Hyun S, Kieft JS, Yu J. An approach to the construction of tailor-made amphiphilic peptides that strongly and selectively bind to hairpin RNA targets. J Am Chem Soc. 2009 Feb 18; 131(6):2224-30. PMID: 19199621; PMCID: PMC2748801.
    40. Hammond JA, Rambo RP, Filbin ME, Kieft JS. Comparison and functional implications of the 3D architectures of viral tRNA-like structures. RNA. 2009 Feb; 15(2):294-307. PMID: 19144910; PMCID: PMC2648705.
    41. Kieft J, Martinez-Salas E. Preface: functionally critical RNA structures found in positive-sense RNA viruses. Virus Res. 2009 Feb; 139(2):135-6. PMID: 18761042.
      View in: PubMed
    42. Kieft JS. Comparing the three-dimensional structures of Dicistroviridae IGR IRES RNAs with other viral RNA structures. Virus Res. 2009 Feb; 139(2):148-56. PMID: 18672012; PMCID: PMC2673954.
    43. Pfingsten JS, Kieft JS. RNA structure-based ribosome recruitment: lessons from the Dicistroviridae intergenic region IRESes. RNA. 2008 Jul; 14(7):1255-63. PMID: 18515544; PMCID: PMC2441983.
    44. Kieft JS. Viral IRES RNA structures and ribosome interactions. Trends Biochem Sci. 2008 Jun; 33(6):274-83. PMID: 18468443; PMCID: PMC2706518.
    45. Costantino DA, Pfingsten JS, Rambo RP, Kieft JS. tRNA-mRNA mimicry drives translation initiation from a viral IRES. Nat Struct Mol Biol. 2008 Jan; 15(1):57-64. PMID: 18157151; PMCID: PMC2748805.
    46. Timmerman SL, Pfingsten JS, Kieft JS, Krushel LA. The 5' leader of the mRNA encoding the mouse neurotrophin receptor TrkB contains two internal ribosomal entry sites that are differentially regulated. PLoS One. 2007 Sep 19; 3(9):e3242. PMID: 18779873; PMCID: PMC2531235.
    47. Keel AY, Rambo RP, Batey RT, Kieft JS. A general strategy to solve the phase problem in RNA crystallography. Structure. 2007 Jul; 15(7):761-72. PMID: 17637337; PMCID: PMC1995091.
    48. Batey RT, Kieft JS. Improved native affinity purification of RNA. RNA. 2007 Aug; 13(8):1384-9. PMID: 17548432; PMCID: PMC1924900.
    49. Pfingsten JS, Costantino DA, Kieft JS. Conservation and diversity among the three-dimensional folds of the Dicistroviridae intergenic region IRESes. J Mol Biol. 2007 Jul 27; 370(5):856-69. PMID: 17544444; PMCID: PMC1974883.
    50. Kieft JS, Costantino DA, Filbin ME, Hammond J, Pfingsten JS. Structural methods for studying IRES function. Methods Enzymol. 2007; 430:333-71. PMID: 17913644.
      View in: PubMed
    51. Pfingsten JS, Costantino DA, Kieft JS. Structural basis for ribosome recruitment and manipulation by a viral IRES RNA. Science. 2006 Dec 01; 314(5804):1450-4. PMID: 17124290; PMCID: PMC2669756.
    52. Kieft JS, Pfingsten JS. Weapons in the molecular arms race. Nat Struct Mol Biol. 2005 Nov; 12(11):938-9. PMID: 16419274.
      View in: PubMed
    53. Costantino D, Kieft JS. A preformed compact ribosome-binding domain in the cricket paralysis-like virus IRES RNAs. RNA. 2005 Mar; 11(3):332-43. PMID: 15701733; PMCID: PMC1370722.
    54. Kieft JS, Batey RT. A general method for rapid and nondenaturing purification of RNAs. RNA. 2004 Jun; 10(6):988-95. PMID: 15146082; PMCID: PMC1370590.
    55. Kieft JS, Zhou K, Grech A, Jubin R, Doudna JA. Crystal structure of an RNA tertiary domain essential to HCV IRES-mediated translation initiation. Nat Struct Biol. 2002 May; 9(5):370-4. PMID: 11927953.
      View in: PubMed
    56. Rijnbrand R, Bredenbeek PJ, Haasnoot PC, Kieft JS, Spaan WJ, Lemon SM. The influence of downstream protein-coding sequence on internal ribosome entry on hepatitis C virus and other flavivirus RNAs. RNA. 2001 Apr; 7(4):585-97. PMID: 11345437; PMCID: PMC1370112.
    57. Spahn CM, Kieft JS, Grassucci RA, Penczek PA, Zhou K, Doudna JA, Frank J. Hepatitis C virus IRES RNA-induced changes in the conformation of the 40s ribosomal subunit. Science. 2001 Mar 09; 291(5510):1959-62. PMID: 11239155.
      View in: PubMed
    58. Kieft JS, Zhou K, Jubin R, Doudna JA. Mechanism of ribosome recruitment by hepatitis C IRES RNA. RNA. 2001 Feb; 7(2):194-206. PMID: 11233977; PMCID: PMC1370078.
    59. Kieft JS, Grech A, Adams P, Doudna JA. Mechanisms of internal ribosome entry in translation initiation. Cold Spring Harb Symp Quant Biol. 2001; 66:277-83. PMID: 12762029.
      View in: PubMed
    60. Jubin R, Vantuno NE, Kieft JS, Murray MG, Doudna JA, Lau JY, Baroudy BM. Hepatitis C virus internal ribosome entry site (IRES) stem loop IIId contains a phylogenetically conserved GGG triplet essential for translation and IRES folding. J Virol. 2000 Nov; 74(22):10430-7. PMID: 11044087; PMCID: PMC110917.
    61. Kieft JS, Zhou K, Jubin R, Murray MG, Lau JY, Doudna JA. The hepatitis C virus internal ribosome entry site adopts an ion-dependent tertiary fold. J Mol Biol. 1999 Sep 24; 292(3):513-29. PMID: 10497018.
      View in: PubMed
    62. Tinoco I, Kieft JS. The ion core in RNA folding. Nat Struct Biol. 1997 Jul; 4(7):509-12. PMID: 9228937.
      View in: PubMed
    63. Kieft JS, Tinoco I. Solution structure of a metal-binding site in the major groove of RNA complexed with cobalt (III) hexammine. Structure. 1997 May 15; 5(5):713-21. PMID: 9195889.
      View in: PubMed
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