 Negative Staining
"Negative Staining" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus,
MeSH (Medical Subject Headings). Descriptors are arranged in a hierarchical structure,
which enables searching at various levels of specificity.
The technique of washing tissue specimens with a concentrated solution of a heavy metal salt and letting it dry. The specimen will be covered with a very thin layer of the metal salt, being excluded in areas where an adsorbed macromolecule is present. The macromolecules allow electrons from the beam of an electron microscope to pass much more readily than the heavy metal; thus, a reversed or negative image of the molecule is created.
| Descriptor ID |
D016624
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| MeSH Number(s) |
E01.370.225.500.620.670.520 E01.370.225.750.600.670.520 E05.200.500.620.670.520 E05.200.750.600.670.520
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| Concept/Terms |
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Below are MeSH descriptors whose meaning is more general than "Negative Staining".
Below are MeSH descriptors whose meaning is more specific than "Negative Staining".
This graph shows the total number of publications written about "Negative Staining" by people in this website by year, and whether "Negative Staining" was a major or minor topic of these publications.
To see the data from this visualization as text, click here.
| Year | Major Topic | Minor Topic | Total |
|---|
| 1996 | 0 | 1 | 1 | | 1998 | 0 | 1 | 1 | | 2002 | 0 | 1 | 1 | | 2007 | 1 | 0 | 1 | | 2008 | 0 | 1 | 1 | | 2010 | 0 | 1 | 1 |
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Below are the most recent publications written about "Negative Staining" by people in Profiles.
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Kirmse R, Bouchet-Marquis C, Page C, Hoenger A. Three-dimensional cryo-electron microscopy on intermediate filaments. Methods Cell Biol. 2010; 96:565-89.
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Small JV, Auinger S, Nemethova M, Koestler S, Goldie KN, Hoenger A, Resch GP. Unravelling the structure of the lamellipodium. J Microsc. 2008 Sep; 231(3):479-85.
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De Carlo S, Boisset N, Hoenger A. High-resolution single-particle 3D analysis on GroEL prepared by cryo-negative staining. Micron. 2008 Oct; 39(7):934-43.
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Resch GP, Goldie KN, Hoenger A, Small JV. Pure F-actin networks are distorted and branched by steps in the critical-point drying method. J Struct Biol. 2002 Mar; 137(3):305-12.
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Steinmetz MO, Hoenger A, Tittmann P, Fuchs KH, Gross H, Aebi U. An atomic model of crystalline actin tubes: combining electron microscopy with X-ray crystallography. J Mol Biol. 1998 May 15; 278(4):703-11.
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Marr KM, Mastronarde DN, Lyon MK. Two-dimensional crystals of photosystem II: biochemical characterization, cryoelectron microscopy and localization of the D1 and cytochrome b559 polypeptides. J Cell Biol. 1996 Mar; 132(5):823-33.
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Ghosh R, Hoenger A, Hardmeyer A, Mihailescu D, Bachofen R, Engel A, Rosenbusch JP. Two-dimensional crystallization of the light-harvesting complex from Rhodospirillum rubrum. J Mol Biol. 1993 May 20; 231(2):501-4.
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