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Marcelo Carlos Sousa

TitleProfessor
InstitutionUniversity of Colorado Boulder
DepartmentChemistry & Biochemistry
Phone303/735-4341
ORCID ORCID Icon0000-0002-0242-3619 Additional info
    Other Positions
    TitleDirector-Faculty
    InstitutionUniversity of Colorado Boulder
    DepartmentChemistry & Biochemistry


    Collapse Research 
    Collapse research activities and funding
    R01AI060841     (SOUSA, MARCELO CARLOS)Jul 1, 2004 - Jun 30, 2009
    NIH/NIAID
    Mechanism of Enzymes Mediating Resistance in Pseudomonas
    Role: Principal Investigator

    R56AI060841     (SOUSA, MARCELO C.)Jul 1, 2004 - Jun 30, 2012
    NIH/NIAID
    Mechanisms of Resistance in Gram-Negative Bacteria
    Role: Principal Investigator

    R01EY019050     (SOUSA, MARCELO C.)May 1, 2009 - Apr 30, 2013
    NIH/NEI
    Molecular Mechanism of Retinal Guanylate Cyclase Activation by GCAPs
    Role: Principal Investigator

    R01AI080709     (SOUSA, MARCELO C.)May 22, 2009 - Jan 31, 2017
    NIH/NIAID
    Transport and Insertion of Outer Membrane Proteins
    Role: Principal Investigator

    Collapse Bibliographic 
    Collapse selected publications
    Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Faculty can login to make corrections and additions.
    List All   |   Timeline
    1. Walder R, LeBlanc MA, Van Patten WJ, Edwards DT, Greenberg JA, Adhikari A, Okoniewski SR, Sullan RMA, Rabuka D, Sousa MC, Perkins TT. Rapid Characterization of a Mechanically Labile a-Helical Protein Enabled by Efficient Site-Specific Bioconjugation. J Am Chem Soc. 2017 Jul 26; 139(29):9867-9875. PMID: 28677396.
      View in: PubMed
    2. Doerner PA, Sousa MC. Extreme Dynamics in the BamA ß-Barrel Seam. Biochemistry. 2017 Jun 20; 56(24):3142-3149. PMID: 28569500.
      View in: PubMed
    3. Warner LR, Gatzeva-Topalova PZ, Doerner PA, Pardi A, Sousa MC. Flexibility in the Periplasmic Domain of BamA Is Important for Function. Structure. 2017 Jan 03; 25(1):94-106. PMID: 27989620.
      View in: PubMed
    4. Bergal HT, Hopkins AH, Metzner SI, Sousa MC. The Structure of a BamA-BamD Fusion Illuminates the Architecture of the ß-Barrel Assembly Machine Core. Structure. 2016 Feb 02; 24(2):243-51. PMID: 26749448; PMCID: PMC4740259 [Available on 02/02/17].
    5. Nordwald EM, Plaks JG, Snell JR, Sousa MC, Kaar JL. Crystallographic Investigation of Imidazolium Ionic Liquid Effects on Enzyme Structure. Chembiochem. 2015 Nov; 16(17):2456-9. PMID: 26388426; PMCID: PMC4827357 [Available on 11/01/16].
    6. Edwards DT, Faulk JK, Sanders AW, Bull MS, Walder R, LeBlanc MA, Sousa MC, Perkins TT. Optimizing 1-µs-Resolution Single-Molecule Force Spectroscopy on a Commercial Atomic Force Microscope. Nano Lett. 2015 Oct 14; 15(10):7091-8. PMID: 26421945; PMCID: PMC4663051 [Available on 10/14/16].
    7. Doerner PA, Sousa MC. Small Angle X-ray Scattering (SAXS) Characterization of the POTRA Domains of BamA. Methods Mol Biol. 2015; 1329:149-55. PMID: 26427682; PMCID: PMC4646726.
    8. Jansen KB, Baker SL, Sousa MC. Crystal structure of BamB bound to a periplasmic domain fragment of BamA, the central component of the ß-barrel assembly machine. J Biol Chem. 2015 Jan 23; 290(4):2126-36. PMID: 25468906; PMCID: PMC4303665.
    9. Turco MM, Sousa MC. The structure and specificity of the type III secretion system effector NleC suggest a DNA mimicry mechanism of substrate recognition. Biochemistry. 2014 Aug 12; 53(31):5131-9. PMID: 25040221; PMCID: PMC4131895.
    10. Lee M, Sousa MC. Structural basis for substrate specificity in ArnB. A key enzyme in the polymyxin resistance pathway of Gram-negative bacteria. Biochemistry. 2014 Feb 04; 53(4):796-805. PMID: 24460375; PMCID: PMC3985747.
    11. Templeton PD, Litman ES, Metzner SI, Ahn NG, Sousa MC. Structure of mediator of RhoA-dependent invasion (MRDI) explains its dual function as a metabolic enzyme and a mediator of cell invasion. Biochemistry. 2013 Aug 20; 52(33):5675-84. PMID: 23859498; PMCID: PMC3831603.
    12. Jansen KB, Baker SL, Sousa MC. Crystal structure of BamB from Pseudomonas aeruginosa and functional evaluation of its conserved structural features. PLoS One. 2012; 7(11):e49749. PMID: 23189157; PMCID: PMC3506653.
    13. Warner LR, Varga K, Lange OF, Baker SL, Baker D, Sousa MC, Pardi A. Structure of the BamC two-domain protein obtained by Rosetta with a limited NMR data set. J Mol Biol. 2011 Aug 05; 411(1):83-95. PMID: 21624375; PMCID: PMC3182476.
    14. Sandoval CM, Baker SL, Jansen K, Metzner SI, Sousa MC. Crystal structure of BamD: an essential component of the ß-Barrel assembly machinery of gram-negative bacteria. J Mol Biol. 2011 Jun 10; 409(3):348-57. PMID: 21463635; PMCID: PMC3098899.
    15. Gatzeva-Topalova PZ, Warner LR, Pardi A, Sousa MC. Structure and flexibility of the complete periplasmic domain of BamA: the protein insertion machine of the outer membrane. Structure. 2010 Nov 10; 18(11):1492-501. PMID: 21070948; PMCID: PMC2991101.
    16. Orban T, Bereta G, Miyagi M, Wang B, Chance MR, Sousa MC, Palczewski K. Conformational changes in guanylate cyclase-activating protein 1 induced by Ca2+ and N-terminal fatty acid acylation. Structure. 2010 Jan 13; 18(1):116-26. PMID: 20152158; PMCID: PMC2822722.
    17. Kabuyama Y, Litman ES, Templeton PD, Metzner SI, Witze ES, Argast GM, Langer SJ, Polvinen K, Shellman Y, Chan D, Shabb JB, Fitzpatrick JE, Resing KA, Sousa MC, Ahn NG. A mediator of Rho-dependent invasion moonlights as a methionine salvage enzyme. Mol Cell Proteomics. 2009 Oct; 8(10):2308-20. PMID: 19620624; PMCID: PMC2758758.
    18. Walton TA, Sandoval CM, Fowler CA, Pardi A, Sousa MC. The cavity-chaperone Skp protects its substrate from aggregation but allows independent folding of substrate domains. Proc Natl Acad Sci U S A. 2009 Feb 10; 106(6):1772-7. PMID: 19181847; PMCID: PMC2644113.
    19. Gatzeva-Topalova PZ, Walton TA, Sousa MC. Crystal structure of YaeT: conformational flexibility and substrate recognition. Structure. 2008 Dec 10; 16(12):1873-81. PMID: 19081063; PMCID: PMC2642521.
    20. Stephen R, Filipek S, Palczewski K, Sousa MC. Ca2+ -dependent regulation of phototransduction. Photochem Photobiol. 2008 Jul-Aug; 84(4):903-10. PMID: 18346093; PMCID: PMC4118144.
    21. Stephen R, Bereta G, Golczak M, Palczewski K, Sousa MC. Stabilizing function for myristoyl group revealed by the crystal structure of a neuronal calcium sensor, guanylate cyclase-activating protein 1. Structure. 2007 Nov; 15(11):1392-402. PMID: 17997965; PMCID: PMC2556213.
    22. Stephen R, Palczewski K, Sousa MC. The crystal structure of GCAP3 suggests molecular mechanism of GCAP-linked cone dystrophies. J Mol Biol. 2006 Jun 02; 359(2):266-75. PMID: 16626734; PMCID: PMC4291230.
    23. Gatzeva-Topalova PZ, May AP, Sousa MC. Structure and mechanism of ArnA: conformational change implies ordered dehydrogenase mechanism in key enzyme for polymyxin resistance. Structure. 2005 Jun; 13(6):929-42. PMID: 15939024; PMCID: PMC2997725.
    24. Gatzeva-Topalova PZ, May AP, Sousa MC. Crystal structure and mechanism of the Escherichia coli ArnA (PmrI) transformylase domain. An enzyme for lipid A modification with 4-amino-4-deoxy-L-arabinose and polymyxin resistance. Biochemistry. 2005 Apr 12; 44(14):5328-38. PMID: 15807526; PMCID: PMC2583347.
    25. Gatzeva-Topalova PZ, May AP, Sousa MC. Crystal structure of Escherichia coli ArnA (PmrI) decarboxylase domain. A key enzyme for lipid A modification with 4-amino-4-deoxy-L-arabinose and polymyxin resistance. Biochemistry. 2004 Oct 26; 43(42):13370-9. PMID: 15491143; PMCID: PMC2680612.
    26. Walton TA, Sousa MC. Crystal structure of Skp, a prefoldin-like chaperone that protects soluble and membrane proteins from aggregation. Mol Cell. 2004 Aug 13; 15(3):367-74. PMID: 15304217.
      View in: PubMed
    27. Ridge KD, Abdulaev NG, Sousa M, Palczewski K. Phototransduction: crystal clear. Trends Biochem Sci. 2003 Sep; 28(9):479-87. PMID: 13678959.
      View in: PubMed
    28. Sousa MC, Kessler BM, Overkleeft HS, McKay DB. Crystal structure of HslUV complexed with a vinyl sulfone inhibitor: corroboration of a proposed mechanism of allosteric activation of HslV by HslU. J Mol Biol. 2002 May 03; 318(3):779-85. PMID: 12054822.
      View in: PubMed
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