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David Nesbitt

TitleProfessor Adjoint
InstitutionUniversity of Colorado Boulder
DepartmentChemistry & Biochemistry
Phone303/492-8857

     Bibliographic 
     selected publications
    Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Faculty can login to make corrections and additions.
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    1. Sengupta A, Sung HL, Nesbitt DJ. Amino Acid Specific Effects on RNA Tertiary Interactions: Single-Molecule Kinetic and Thermodynamic Studies. J Phys Chem B. 2016 Oct 10.
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    2. Efros AL, Nesbitt DJ. Origin and control of blinking in quantum dots. Nat Nanotechnol. 2016 Aug 03; 11(8):661-71.
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    3. Chang CH, Nesbitt DJ. High resolution spectroscopy of jet cooled phenyl radical: The ?1 and ?2 a1 symmetry C-H stretching modes. J Chem Phys. 2016 Jul 28; 145(4):044304.
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    4. Holmstrom ED, Nesbitt DJ. Biophysical Insights from Temperature-Dependent Single-Molecule Förster Resonance Energy Transfer. Annu Rev Phys Chem. 2016 May 27; 67:441-65.
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    5. Polaski JT, Holmstrom ED, Nesbitt DJ, Batey RT. Mechanistic Insights into Cofactor-Dependent Coupling of RNA Folding and mRNA Transcription/Translation by a Cobalamin Riboswitch. Cell Rep. 2016 05 03; 15(5):1100-10.
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    6. Chang CH, Agarwal J, Allen WD, Nesbitt DJ. Sub-Doppler infrared spectroscopy and formation dynamics of triacetylene in a slit supersonic expansion. J Chem Phys. 2016 Feb 21; 144(7):074301.
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    7. Vieweger M, Holmstrom ED, Nesbitt DJ. Single-Molecule FRET Reveals Three Conformations for the TLS Domain of Brome Mosaic Virus Genome. Biophys J. 2015 Dec 15; 109(12):2625-36.
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    8. Early KT, Nesbitt DJ. Ultrafast Laser Studies of Two-Photon Excited Fluorescence Intermittency in Single CdSe/ZnS Quantum Dots. Nano Lett. 2015 Dec 09; 15(12):7781-7.
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    9. Chang CH, Nesbitt DJ. Sub-Doppler infrared spectroscopy of propargyl radical (H2CCCH) in a slit supersonic expansion. J Chem Phys. 2015 Jun 28; 142(24):244313.
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    10. Chang CH, Nesbitt DJ. Spectroscopy and Dynamics of Jet-Cooled Polyynes in a Slit Supersonic Discharge: Sub-Doppler Infrared Studies of Diacetylene HCCCCH. J Phys Chem A. 2015 Jul 16; 119(28):7940-50.
      View in: PubMed
    11. Liu Y, Holmstrom E, Zhang J, Yu P, Wang J, Dyba MA, Chen D, Ying J, Lockett S, Nesbitt DJ, Ferré-D'Amaré AR, Sousa R, Stagno JR, Wang YX. Synthesis and applications of RNAs with position-selective labelling and mosaic composition. Nature. 2015 Jun 18; 522(7556):368-72.
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    12. Holmstrom ED, Dupuis NF, Nesbitt DJ. Kinetic and thermodynamic origins of osmolyte-influenced nucleic acid folding. J Phys Chem B. 2015 Mar 05; 119(9):3687-96.
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    13. Holmstrom ED, Polaski JT, Batey RT, Nesbitt DJ. Single-molecule conformational dynamics of a biologically functional hydroxocobalamin riboswitch. J Am Chem Soc. 2014 Dec 03; 136(48):16832-43.
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    14. Dupuis NF, Holmstrom ED, Nesbitt DJ. Molecular-crowding effects on single-molecule RNA folding/unfolding thermodynamics and kinetics. Proc Natl Acad Sci U S A. 2014 Jun 10; 111(23):8464-9.
      View in: PubMed
    15. Holmstrom ED, Nesbitt DJ. Single-molecule fluorescence resonance energy transfer studies of the human telomerase RNA pseudoknot: temperature-/urea-dependent folding kinetics and thermodynamics. J Phys Chem B. 2014 Apr 10; 118(14):3853-63.
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    16. Holmstrom ED, Dupuis NF, Nesbitt DJ. Pulsed IR heating studies of single-molecule DNA duplex dissociation kinetics and thermodynamics. Biophys J. 2014 Jan 07; 106(1):220-31.
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    17. Early KT, Nesbitt DJ. Size-dependent photoionization in single CdSe/ZnS nanocrystals. Nano Lett. 2013 Oct 09; 13(10):4844-9.
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    18. Dupuis NF, Holmstrom ED, Nesbitt DJ. Single-molecule kinetics reveal cation-promoted DNA duplex formation through ordering of single-stranded helices. Biophys J. 2013 Aug 06; 105(3):756-66.
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    19. Fiore JL, Nesbitt DJ. An RNA folding motif: GNRA tetraloop-receptor interactions. Q Rev Biophys. 2013 Aug; 46(3):223-64.
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    20. Chang CH, Buckingham GT, Nesbitt DJ. Sub-Doppler spectroscopy of the trans-HOCO radical in the OH stretching mode. J Phys Chem A. 2013 Dec 19; 117(50):13255-64.
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    21. Buckingham GT, Chang CH, Nesbitt DJ. High-resolution rovibrational spectroscopy of jet-cooled phenyl radical: the ?19 out-of-phase symmetric CH stretch. J Phys Chem A. 2013 Oct 03; 117(39):10047-57.
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    22. Roberts MA, Sharp-Williams EN, Nesbitt DJ. High-resolution direct-absorption spectroscopy of hydroxymethyl radical in the CH symmetric stretching region. J Phys Chem A. 2013 Aug 15; 117(32):7042-9.
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    23. Grubisic A, Schweikhard V, Baker TA, Nesbitt DJ. Multiphoton photoelectron emission microscopy of single Au nanorods: combined experimental and theoretical study of rod morphology and dielectric environment on localized surface plasmon resonances. Phys Chem Chem Phys. 2013 Jul 14; 15(26):10616-27.
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    24. Grubisic A, Schweikhard V, Baker TA, Nesbitt DJ. Coherent multiphoton photoelectron emission from single au nanorods: the critical role of plasmonic electric near-field enhancement. ACS Nano. 2013 Jan 22; 7(1):87-99.
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    25. Fiegland LR, Garst AD, Batey RT, Nesbitt DJ. Single-molecule studies of the lysine riboswitch reveal effector-dependent conformational dynamics of the aptamer domain. Biochemistry. 2012 Nov 13; 51(45):9223-33.
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    26. Nesbitt DJ. Toward state-to-state dynamics in ultracold collisions: lessons from high-resolution spectroscopy of weakly bound molecular complexes. Chem Rev. 2012 Sep 12; 112(9):5062-72.
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    27. Ziemkiewicz MP, Pluetzer C, Nesbitt DJ, Scribano Y, Faure A, van der Avoird A. Overtone vibrational spectroscopy in H2-H2O complexes: a combined high level theoretical ab initio, dynamical and experimental study. J Chem Phys. 2012 Aug 28; 137(8):084301.
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    28. Grubisic A, Ringe E, Cobley CM, Xia Y, Marks LD, Van Duyne RP, Nesbitt DJ. Plasmonic near-electric field enhancement effects in ultrafast photoelectron emission: correlated spatial and laser polarization microscopy studies of individual Ag nanocubes. Nano Lett. 2012 Sep 12; 12(9):4823-9.
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    29. Fiore JL, Holmstrom ED, Fiegland LR, Hodak JH, Nesbitt DJ. The role of counterion valence and size in GAAA tetraloop-receptor docking/undocking kinetics. J Mol Biol. 2012 Oct 19; 423(2):198-216.
      View in: PubMed
    30. Roberts MA, Savage C, Dong F, Sharp-Williams EN, McCoy AB, Nesbitt DJ. Sub-Doppler infrared spectroscopy of CH2D radical in a slit supersonic jet: isotopic symmetry breaking in the CH stretching manifold. J Chem Phys. 2012 Jun 21; 136(23):234308.
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    31. Holmstrom ED, Fiore JL, Nesbitt DJ. Thermodynamic origins of monovalent facilitated RNA folding. Biochemistry. 2012 May 08; 51(18):3732-43.
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    32. Li X, Schatz GC, Nesbitt DJ. Anion effects in the scattering of CO2 from the room-temperature ionic liquids [bmim][BF4] and [bmim][Tf2N]: insights from quantum mechanics/molecular mechanics trajectories. J Phys Chem B. 2012 Mar 22; 116(11):3587-602.
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    33. Fiore JL, Holmstrom ED, Nesbitt DJ. Entropic origin of Mg2+-facilitated RNA folding. Proc Natl Acad Sci U S A. 2012 Feb 21; 109(8):2902-7.
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    34. Sharma AR, Bowman JM, Nesbitt DJ. Large-amplitude dynamics in vinyl radical: the role of quantum tunneling as an isomerization mechanism. J Chem Phys. 2012 Jan 21; 136(3):034305.
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    35. Gisler AW, Nesbitt DJ. On probing ions at the gas-liquid interface by quantum state-resolved molecular beam scattering: the curious incident of the cation in the night time. Faraday Discuss. 2012; 157:297-305; discussion 375-98.
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    36. Sharp-Williams EN, Roberts MA, Nesbitt DJ. Dark state vibronic coupling in the Ã(2?) ? ?X(2S+) band of ethynyl radical via high resolution infrared absorption spectroscopy. Phys Chem Chem Phys. 2011 Oct 21; 13(39):17474-83.
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    37. Roscioli JR, Nesbitt DJ. Quantum state resolved scattering from room-temperature ionic liquids: the role of cation versus anion structure at the interface. J Phys Chem A. 2011 Sep 01; 115(34):9764-73.
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    38. Ziemkiewicz MP, Roscioli JR, Nesbitt DJ. State-to-state dynamics at the gas-liquid metal interface: rotationally and electronically inelastic scattering of NO[2?(1/2)(0.5)] from molten gallium. J Chem Phys. 2011 Jun 21; 134(23):234703.
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    39. Schweikhard V, Grubisic A, Baker TA, Thomann I, Nesbitt DJ. Polarization-dependent scanning photoionization microscopy: ultrafast plasmon-mediated electron ejection dynamics in single Au nanorods. ACS Nano. 2011 May 24; 5(5):3724-35.
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    40. Casavecchia P, Brouard M, Costes M, Nesbitt D, Bieske E, Kable S. Molecular collision dynamics. Phys Chem Chem Phys. 2011 May 14; 13(18):8073-4.
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    41. Sharp-Williams EN, Roberts MA, Nesbitt DJ. High resolution slit-jet infrared spectroscopy of ethynyl radical: 2?-2S+ vibronic bands with sub-Doppler resolution. J Chem Phys. 2011 Feb 14; 134(6):064314.
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    42. van der Avoird A, Nesbitt DJ. Rovibrational states of the H2O-H2 complex: an ab initio calculation. J Chem Phys. 2011 Jan 28; 134(4):044314.
      View in: PubMed
    43. Roscioli JR, Nesbitt DJ. Quantum state resolved velocity-map imaging spectroscopy: a new tool for collision dynamics at gas/self-assembled monolayer interfaces. Faraday Discuss. 2011; 150:471-9; discussion 505-32.
      View in: PubMed
    44. Perkins BG, Nesbitt DJ. High resolution Dopplerimetry of correlated angular and quantum state-resolved CO(2) scattering dynamics at the gas-liquid interface. Phys Chem Chem Phys. 2010 Nov 14; 12(42):14294-308.
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    45. Nesbitt DJ, Suhm MA. Chemical dynamics of large amplitude motion. Phys Chem Chem Phys. 2010 Aug 01; 12(29):8151.
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    46. Terrill K, Nesbitt DJ. Ab initio anharmonic vibrational frequency predictions for linear proton-bound complexes OC-H(+)-CO and N(2)-H(+)-N(2). Phys Chem Chem Phys. 2010 Aug 01; 12(29):8311-22.
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    47. Müller HS, Dong F, Nesbitt DJ, Furuya T, Saito S. Tunneling dynamics and spectroscopic parameters of monodeuterated hydronium, H(2)DO(+), from a combined analysis of infrared and sub-millimeter spectra. Phys Chem Chem Phys. 2010 Aug 01; 12(29):8362-72.
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    48. Perkins BG, Nesbitt DJ. Stereodynamics at the gas-liquid interface: orientation and alignment of CO2 scattered from perfluorinated liquid surfaces. J Phys Chem A. 2010 Jan 28; 114(3):1398-410.
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    49. Holmstrom ED, Nesbitt DJ. Real-Time Infrared Overtone Laser Control of Temperature in Picoliter H(2)O Samples: "Nanobathtubs" for Single Molecule Microscopy. J Phys Chem Lett. 2010; 1(15):2264-2268.
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    50. Ni KK, Ospelkaus S, Nesbitt DJ, Ye J, Jin DS. A dipolar gas of ultracold molecules. Phys Chem Chem Phys. 2009 Nov 14; 11(42):9626-39.
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    51. Ziemkiewicz M, Nesbitt DJ. Nonadiabatic reactive scattering in atom + triatom systems: nascent rovibronic distributions in F + H2O --> HF + OH. J Chem Phys. 2009 Aug 07; 131(5):054309.
      View in: PubMed
    52. Nogueira JJ, Vázquez SA, Mazyar OA, Hase WL, Perkins BG, Nesbitt DJ, Martínez-Núñez E. Dynamics of CO2 scattering off a perfluorinated self-assembled monolayer. Influence of the incident collision energy, mass effects, and use of different surface models. J Phys Chem A. 2009 Apr 23; 113(16):3850-65.
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    53. Perkins BG, Nesbitt DJ. Toward three-dimensional quantum state-resolved collision dynamics at the gas-liquid interface: theoretical investigation of incident angle. J Phys Chem A. 2009 Apr 23; 113(16):4613-25.
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    54. Fiore JL, Kraemer B, Koberling F, Edmann R, Nesbitt DJ. Enthalpy-driven RNA folding: single-molecule thermodynamics of tetraloop-receptor tertiary interaction. Biochemistry. 2009 Mar 24; 48(11):2550-8.
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    55. Peterson JJ, Nesbitt DJ. Modified power law behavior in quantum dot blinking: a novel role for biexcitons and auger ionization. Nano Lett. 2009 Jan; 9(1):338-45.
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    56. Zolot AM, Dagdigian PJ, Nesbitt DJ. Quantum-state resolved reactive scattering at the gas-liquid interface: F+squalane (C30H62) dynamics via high-resolution infrared absorption of nascent HF(v,J). J Chem Phys. 2008 Nov 21; 129(19):194705.
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    57. Zolot AM, Nesbitt DJ. Crossed jet reactive scattering dynamics of F+H(2)O-->HF(v,J)+OH:HF(v,J) product quantum state distributions under single-collision conditions. J Chem Phys. 2008 Nov 14; 129(18):184305.
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    58. Sharp EN, Roberts MA, Nesbitt DJ. Rotationally resolved infrared spectroscopy of a jet-cooled phenyl radical in the gas phase. Phys Chem Chem Phys. 2008 Nov 28; 10(44):6592-6.
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    59. Taatjes CA, Osborn DL, Nesbitt DJ, Zwier TS. Introductory tribute to Stephen R. Leone. J Phys Chem A. 2008 Oct 02; 112(39):9167-8.
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    60. Perkins BG, Nesbitt DJ. Correlated angular and quantum state-resolved CO2 scattering dynamics at the gas-liquid interface. J Phys Chem A. 2008 Oct 02; 112(39):9324-35.
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    61. Perkins BG, Nesbitt DJ. Stereodynamics in state-resolved scattering at the gas-liquid interface. Proc Natl Acad Sci U S A. 2008 Sep 02; 105(35):12684-9.
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    62. Fiore JL, Hodak JH, Piestert O, Downey CD, Nesbitt DJ. Monovalent and divalent promoted GAAA tetraloop-receptor tertiary interactions from freely diffusing single-molecule studies. Biophys J. 2008 Oct; 95(8):3892-905.
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    63. Deskevich MP, McCoy AB, Hutson JM, Nesbitt DJ. Large-amplitude quantum mechanics in polyatomic hydrides. II. A particle-on-a-sphere model for XH(n) (n=4,5). J Chem Phys. 2008 Mar 07; 128(9):094306.
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    64. Lique F, Alexander MH, Li G, Werner HJ, Nizkorodov SA, Harper WW, Nesbitt DJ. Evidence for excited spin-orbit state reaction dynamics in F+H2: theory and experiment. J Chem Phys. 2008 Feb 28; 128(8):084313.
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    65. Nesbitt DJ, Dong F. Ab initio large-amplitude quantum-tunneling dynamics in vinyl radical: a vibrationally adiabatic approach. Phys Chem Chem Phys. 2008 Apr 21; 10(15):2113-22.
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    66. Dong F, Roberts M, Nesbitt DJ. High-resolution infrared spectroscopy of jet-cooled vinyl radical: symmetric CH2 stretch excitation and tunneling dynamics. J Chem Phys. 2008 Jan 28; 128(4):044305.
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    67. Fomenko V, Nesbitt DJ. Solution control of radiative and nonradiative lifetimes: a novel contribution to quantum dot blinking suppression. Nano Lett. 2008 Jan; 8(1):287-93.
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    68. Nesbitt DJ. Photonics: charge of the light brigade. Nature. 2007 Dec 20; 450(7173):1172-3.
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    69. Perkins BG, Nesbitt DJ. Quantum state-resolved CO2 collisions at the gas-liquid interface: surface temperature-dependent scattering dynamics. J Phys Chem B. 2008 Jan 17; 112(2):507-19.
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    70. Zolot AM, Nesbitt DJ. Quantum state resolved scattering dynamics of F+HCl-->HF(v,J)+Cl. J Chem Phys. 2007 Sep 21; 127(11):114319.
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    71. Perkins BG, Nesbitt DJ. Quantum-state-resolved CO2 scattering dynamics at the gas-liquid interface: dependence on incident angle. J Phys Chem A. 2007 Aug 09; 111(31):7420-30.
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    72. Monti OL, Baker TA, Nesbitt DJ. Imaging nanostructures with scanning photoionization microscopy. J Chem Phys. 2006 Oct 21; 125(15):154709.
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    73. Dong F, Nesbitt DJ. Jet cooled spectroscopy of H2DO+: Barrier heights and isotope-dependent tunneling dynamics from H3O+ to D3O+. J Chem Phys. 2006 Oct 14; 125(14):144311.
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    74. Perkins BG, Nesbitt DJ. Quantum-state-resolved CO2 scattering dynamics at the gas-liquid interface: incident collision energy and liquid dependence. J Phys Chem B. 2006 Aug 31; 110(34):17126-37.
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    75. Whitney ES, Haeber T, Schuder MD, Blair AC, Nesbitt DJ. High-resolution infrared studies in slit supersonic discharges: CH2 stretch excitation of jet-cooled CH2Cl radical. J Chem Phys. 2006 Aug 07; 125(5):054303.
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    76. Whitney ES, Dong F, Nesbitt DJ. Jet-cooled infrared spectroscopy in slit supersonic discharges: symmetric and antisymmetric CH2 stretching modes of fluoromethyl (CH2F) radical. J Chem Phys. 2006 Aug 07; 125(5):054304.
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    77. Zolot AM, Harper WW, Perkins BG, Dagdigian PJ, Nesbitt DJ. Quantum-state resolved reaction dynamics at the gas-liquid interface: direct absorption detection of HF(v,J) product from F(2P)+squalane. J Chem Phys. 2006 Jul 14; 125(2):21101.
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    78. Deskevich MP, Hayes MY, Takahashi K, Skodje RT, Nesbitt DJ. Multireference configuration interaction calculations for the F(2P)+HCl-->HF+Cl(2P) reaction: a correlation scaled ground state (1 2A') potential energy surface. J Chem Phys. 2006 Jun 14; 124(22):224303.
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    79. Downey CD, Fiore JL, Stoddard CD, Hodak JH, Nesbitt DJ, Pardi A. Metal ion dependence, thermodynamics, and kinetics for intramolecular docking of a GAAA tetraloop and receptor connected by a flexible linker. Biochemistry. 2006 Mar 21; 45(11):3664-73.
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    80. Dong F, Davis S, Nesbitt DJ. Slit discharge IR spectroscopy of a jet-cooled cyclopropyl radical: structure and intramolecular tunneling dynamics. J Phys Chem A. 2006 Mar 09; 110(9):3059-70.
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    81. Häber T, Blair AC, Nesbitt DJ, Schuder MD. CH stretch/internal rotor dynamics in ethyl radical: high-resolution spectroscopy in the CH3-stretch manifold. J Chem Phys. 2006 Feb 07; 124(5):054316.
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    82. Hayes MY, Deskevich MP, Nesbitt DJ, Takahashi K, Skodje RT. A simple picture for the rotational enhancement of the rate for the F + HCl --> HF + Cl reaction: a dynamical study using a new ab initio potential energy surface. J Phys Chem A. 2006 Jan 19; 110(2):436-44.
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    83. Huang X, McCoy AB, Bowman JM, Johnson LM, Savage C, Dong F, Nesbitt DJ. Quantum deconstruction of the infrared spectrum of CH5+. Science. 2006 Jan 06; 311(5757):60-3.
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    84. Ziemkiewicz M, Wojcik M, Nesbitt DJ. Direct evidence for nonadiabatic dynamics in atom+polyatom reactions: crossed-jet laser studies of F+D2O-->DF+OD. J Chem Phys. 2005 Dec 08; 123(22):224307.
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    85. Perkins BG, Häber T, Nesbitt DJ. Quantum state-resolved energy transfer dynamics at gas-liquid interfaces: IR laser studies of CO2 scattering from perfluorinated liquids. J Phys Chem B. 2005 Sep 01; 109(34):16396-405.
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    86. Deskevich MP, Nesbitt DJ. Large amplitude quantum mechanics in polyatomic hydrides. I. A particles-on-a-sphere model for XH(n). J Chem Phys. 2005 Aug 22; 123(8):084304.
      View in: PubMed
    87. Hodak JH, Downey CD, Fiore JL, Pardi A, Nesbitt DJ. Docking kinetics and equilibrium of a GAAA tetraloop-receptor motif probed by single-molecule FRET. Proc Natl Acad Sci U S A. 2005 Jul 26; 102(30):10505-10.
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    88. Dong F, Uy D, Davis S, Child M, Nesbitt DJ. Supersonically cooled hydronium ions in a slit-jet discharge: high-resolution infrared spectroscopy and tunneling dynamics of HD2O+. J Chem Phys. 2005 Jun 08; 122(22):224301.
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    89. Nizkorodov SA, Ziemkiewicz M, Nesbitt DJ, Knight AE. Overtone spectroscopy of H2O clusters in the V(OH) = 2 manifold: infrared-ultraviolet vibrationally mediated dissociation studies. J Chem Phys. 2005 May 15; 122(19):194316.
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    90. Whitney ES, Zolot AM, McCoy AB, Francisco JS, Nesbitt DJ. Reactive scattering dynamics in atom+polyatomic systems: F+C2H6-->HF(v,J)+C2H5. J Chem Phys. 2005 Mar 22; 122(12):124310.
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    91. Fárník M, Nesbitt DJ. Intramolecular energy transfer between oriented chromophores: high-resolution infrared spectroscopy of HCl trimer. J Chem Phys. 2004 Dec 22; 121(24):12386-95.
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    92. Votava O, Mackenzie SR, Nesbitt DJ. Intracluster stereochemistry in van der Waals complexes: steric effects in ultraviolet photodissociation of state-selected Ar-HOD/H2O. J Chem Phys. 2004 May 08; 120(18):8443-52.
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    93. Deskevich MP, Nesbitt DJ, Werner HJ. Dynamically weighted multiconfiguration self-consistent field: multistate calculations for F+H2O-->HF+OH reaction paths. J Chem Phys. 2004 Apr 22; 120(16):7281-9.
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    94. Bohn JL, Nesbitt DJ, Gallagher A. Field enhancement in apertureless near-field scanning optical microscopy. J Opt Soc Am A Opt Image Sci Vis. 2001 Dec; 18(12):2998-3006.
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    95. Mori K, Ando A, Gehlbach P, Nesbitt D, Takahashi K, Goldsteen D, Penn M, Chen CT, Mori K, Melia M, Phipps S, Moffat D, Brazzell K, Liau G, Dixon KH, Campochiaro PA. Inhibition of choroidal neovascularization by intravenous injection of adenoviral vectors expressing secretable endostatin. Am J Pathol. 2001 Jul; 159(1):313-20.
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    96. Fárník M, Davis S, Nesbitt DJ. High-resolution IR studies of hydrogen bonded clusters: large amplitude dynamics in (HCl)n. Faraday Discuss. 2001; (118):63-78; discussion 109-19.
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    97. Plusquellic DF, Votava O, Nesbitt DJ. Absolute frequency stabilization of an injection-seeded optical parametric oscillator. Appl Opt. 1996 Mar 20; 35(9):1464-72.
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