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Amy E Palmer

TitleAssociate Professor
InstitutionUniversity of Colorado Boulder
DepartmentChemistry & Biochemistry
Phone303/492-1945

    Collapse Research 
    Collapse research activities and funding
    F32GM067488     (PALMER, AMY E)Mar 1, 2003 - Feb 28, 2005
    NIH/NIGMS
    New Strategy for Development of Novel FRET-based sensors
    Role: Principal Investigator

    R01GM084027     (PALMER, AMY E)May 1, 2008 - May 31, 2017
    NIH/NIGMS
    Genetically Encoded Sensors Shed Light on Zinc Homeostasis
    Role: Principal Investigator

    R01GM083849     (PALMER, AMY E)Jul 1, 2008 - Jun 30, 2013
    NIH/NIGMS
    Microfluidics-based Selections for the Optimization of Red Flourescent Proteins
    Role: Principal Investigator

    R01GM105997     (AHN, NATALIE G)Jul 1, 2013 - Apr 30, 2017
    NIH/NIGMS
    Technologies to Define and Map Novel Interorganelle Macromolecular Interactions
    Role: Co-Principal Investigator

    R13GM112419     (KNOELL, DAREN LEE)Jul 10, 2014 - Jun 30, 2015
    NIH/NIGMS
    International Society for Zinc Biology Conference
    Role: Co-Principal Investigator

    DP1GM114863     (PALMER, AMY E)Sep 30, 2014 - Jul 31, 2019
    NIH/NIGMS
    Regulation of Cell Signaling by Transition Metal Dynamics
    Role: Principal Investigator

    Collapse Bibliographic 
    Collapse selected publications
    Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Faculty can login to make corrections and additions.
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    1. Young AM, Minson M, McQuate SE, Palmer AE. Optimized Fluorescence Complementation Platform for Visualizing Salmonella Effector Proteins Reveals Distinctly Different Intracellular Niches in Different Cell Types. ACS Infect Dis. 2017 Jun 09. PMID: 28551989.
      View in: PubMed
    2. Carpenter MC, Palmer AE. Native and engineered sensors for Ca2+ and Zn2+: lessons from calmodulin and MTF1. Essays Biochem. 2017 May 09; 61(2):237-243. PMID: 28487400.
      View in: PubMed
    3. Lauinger L, Li J, Shostak A, Cemel IA, Ha N, Zhang Y, Merkl PE, Obermeyer S, Stankovic-Valentin N, Schafmeier T, Wever WJ, Bowers AA, Carter KP, Palmer AE, Tschochner H, Melchior F, Deshaies RJ, Brunner M, Diernfellner A. Thiolutin is a zinc chelator that inhibits the Rpn11 and other JAMM metalloproteases. Nat Chem Biol. 2017 Jul; 13(7):709-714. PMID: 28459440.
      View in: PubMed
    4. Mo GC, Ross B, Hertel F, Manna P, Yang X, Greenwald E, Booth C, Plummer AM, Tenner B, Chen Z, Wang Y, Kennedy EJ, Cole PA, Fleming KG, Palmer A, Jimenez R, Xiao J, Dedecker P, Zhang J. Genetically encoded biosensors for visualizing live-cell biochemical activity at super-resolution. Nat Methods. 2017 Apr; 14(4):427-434. PMID: 28288122.
      View in: PubMed
    5. Sanford L, Palmer A. Recent Advances in Development of Genetically Encoded Fluorescent Sensors. Methods Enzymol. 2017; 589:1-49. PMID: 28336060.
      View in: PubMed
    6. Li J, Yakushi T, Parlati F, Mackinnon AL, Perez C, Ma Y, Carter KP, Colayco S, Magnuson G, Brown B, Nguyen K, Vasile S, Suyama E, Smith LH, Sergienko E, Pinkerton AB, Chung TDY, Palmer AE, Pass I, Hess S, Cohen SM, Deshaies RJ. Capzimin is a potent and specific inhibitor of proteasome isopeptidase Rpn11. Nat Chem Biol. 2017 May; 13(5):486-493. PMID: 28244987.
      View in: PubMed
    7. Fiedler BL, Van Buskirk S, Carter KP, Qin Y, Carpenter MC, Palmer AE, Jimenez R. Droplet Microfluidic Flow Cytometer For Sorting On Transient Cellular Responses Of Genetically-Encoded Sensors. Anal Chem. 2017 Jan 03; 89(1):711-719. PMID: 27959493.
      View in: PubMed
    8. Specht EA, Braselmann E, Palmer AE. A Critical and Comparative Review of Fluorescent Tools for Live-Cell Imaging. Annu Rev Physiol. 2017 Feb 10; 79:93-117. PMID: 27860833.
      View in: PubMed
    9. Rodriguez EA, Campbell RE, Lin JY, Lin MZ, Miyawaki A, Palmer AE, Shu X, Zhang J, Tsien RY. The Growing and Glowing Toolbox of Fluorescent and Photoactive Proteins. Trends Biochem Sci. 2017 Feb; 42(2):111-129. PMID: 27814948.
      View in: PubMed
    10. Palmer AE, Zhang J. Roger Y. Tsien 1952-2016. Nat Chem Biol. 2016 Nov; 12(11):887. PMID: 27669418.
      View in: PubMed
    11. Carpenter MC, Lo MN, Palmer AE. Techniques for measuring cellular zinc. Arch Biochem Biophys. 2016 Dec 01; 611:20-29. PMID: 27580940.
      View in: PubMed
    12. Qin Y, Sammond DW, Braselmann E, Carpenter MC, Palmer AE. Development of an Optical Zn2+ Probe Based on a Single Fluorescent Protein. ACS Chem Biol. 2016 Oct 21; 11(10):2744-2751. PMID: 27467056.
      View in: PubMed
    13. Carpenter MC, Palmer AE. Unraveling the mystery of the ring: Tracking heme dynamics in living cells. Proc Natl Acad Sci U S A. 2016 07 05; 113(27):7296-7. PMID: 27342864; PMCID: PMC4941497 [Available on 01/05/17].
    14. Dean KM, Davis LM, Lubbeck JL, Manna P, Friis P, Palmer AE, Jimenez R. High-speed multiparameter photophysical analyses of fluorophore libraries. Anal Chem. 2015; 87(10):5026-30. PMID: 25898152; PMCID: PMC4648346.
    15. Kim TJ, Joo C, Seong J, Vafabakhsh R, Botvinick EL, Berns MW, Palmer AE, Wang N, Ha T, Jakobsson E, Sun J, Wang Y. Distinct mechanisms regulating mechanical force-induced Ca²? signals at the plasma membrane and the ER in human MSCs. Elife. 2015 Feb 10; 4:e04876. PMID: 25667984; PMCID: PMC4337650.
    16. Dean KM, Lubbeck JL, Davis LM, Regmi CK, Chapagain PP, Gerstman BS, Jimenez R, Palmer AE. Microfluidics-based selection of red-fluorescent proteins with decreased rates of photobleaching. Integr Biol (Camb). 2015 Feb; 7(2):263-73. PMID: 25477249; PMCID: PMC4323946.
    17. Carter KP, Palmer AE. Metallobiology: zinc differently. Nat Chem. 2015 Feb; 7(2):96-7. PMID: 25615659.
      View in: PubMed
    18. Park JG, Palmer AE. Verifying the function and localization of genetically encoded Ca2+ sensors and converting FRET ratios to Ca2+ concentrations. Cold Spring Harb Protoc. 2015 Jan 05; 2015(1):pdb.prot076547. PMID: 25561614; PMCID: PMC4968932.
    19. Park JG, Palmer AE. Measuring the in situ Kd of a genetically encoded Ca2+ sensor. Cold Spring Harb Protoc. 2015 Jan 05; 2015(1):pdb.prot076554. PMID: 25561615; PMCID: PMC5012106.
    20. Park JG, Palmer AE. Properties and use of genetically encoded FRET sensors for cytosolic and organellar Ca2+ measurements. Cold Spring Harb Protoc. 2015 Jan 05; 2015(1):pdb.top066043. PMID: 25561625; PMCID: PMC4968205.
    21. Farnsworth NL, Mead BE, Antunez LR, Palmer AE, Bryant SJ. Ionic osmolytes and intracellular calcium regulate tissue production in chondrocytes cultured in a 3D charged hydrogel. Matrix Biol. 2014 Nov; 40:17-26. PMID: 25128592.
      View in: PubMed
    22. Dean KM, Palmer AE. Advances in fluorescence labeling strategies for dynamic cellular imaging. Nat Chem Biol. 2014 Jul; 10(7):512-23. PMID: 24937069; PMCID: PMC4248787.
    23. Carter KP, Young AM, Palmer AE. Fluorescent sensors for measuring metal ions in living systems. Chem Rev. 2014 Apr 23; 114(8):4564-601. PMID: 24588137; PMCID: PMC4096685.
    24. Park JG, Palmer AE. Quantitative measurement of Ca2+ and Zn2+ in mammalian cells using genetically encoded fluorescent biosensors. Methods Mol Biol. 2014; 1071:29-47. PMID: 24052378; PMCID: PMC4051312.
    25. Stavru F, Palmer AE, Wang C, Youle RJ, Cossart P. Atypical mitochondrial fission upon bacterial infection. Proc Natl Acad Sci U S A. 2013 Oct 01; 110(40):16003-8. PMID: 24043775; PMCID: PMC3791707.
    26. Qin Y, Miranda JG, Stoddard CI, Dean KM, Galati DF, Palmer AE. Direct comparison of a genetically encoded sensor and small molecule indicator: implications for quantification of cytosolic Zn(2+). ACS Chem Biol. 2013 Nov 15; 8(11):2366-71. PMID: 23992616; PMCID: PMC4022295.
    27. Davis LM, Lubbeck JL, Dean KM, Palmer AE, Jimenez R. Microfluidic cell sorter for use in developing red fluorescent proteins with improved photostability. Lab Chip. 2013 Jun 21; 13(12):2320-7. PMID: 23636097; PMCID: PMC4047792.
    28. Jeong J, Walker JM, Wang F, Park JG, Palmer AE, Giunta C, Rohrbach M, Steinmann B, Eide DJ. Promotion of vesicular zinc efflux by ZIP13 and its implications for spondylocheiro dysplastic Ehlers-Danlos syndrome. Proc Natl Acad Sci U S A. 2012 Dec 18; 109(51):E3530-8. PMID: 23213233; PMCID: PMC3529093.
    29. Miranda JG, Weaver AL, Qin Y, Park JG, Stoddard CI, Lin MZ, Palmer AE. New alternately colored FRET sensors for simultaneous monitoring of Zn²? in multiple cellular locations. PLoS One. 2012; 7(11):e49371. PMID: 23173058; PMCID: PMC3500285.
    30. Geng X, Huang C, Qin Y, McCombs JE, Yuan Q, Harry BL, Palmer AE, Xia NS, Xue D. Hepatitis B virus X protein targets Bcl-2 proteins to increase intracellular calcium, required for virus replication and cell death induction. Proc Natl Acad Sci U S A. 2012 Nov 06; 109(45):18471-6. PMID: 23091012; PMCID: PMC3494919.
    31. Park JG, Qin Y, Galati DF, Palmer AE. New sensors for quantitative measurement of mitochondrial Zn(2+). ACS Chem Biol. 2012 Oct 19; 7(10):1636-40. PMID: 22850482; PMCID: PMC3477250.
    32. West DC, Qin Y, Peterson QP, Thomas DL, Palchaudhuri R, Morrison KC, Lucas PW, Palmer AE, Fan TM, Hergenrother PJ. Differential effects of procaspase-3 activating compounds in the induction of cancer cell death. Mol Pharm. 2012 May 07; 9(5):1425-34. PMID: 22486564; PMCID: PMC3348238.
    33. Dean KM, Qin Y, Palmer AE. Visualizing metal ions in cells: an overview of analytical techniques, approaches, and probes. Biochim Biophys Acta. 2012 Sep; 1823(9):1406-15. PMID: 22521452; PMCID: PMC3408866.
    34. Lubbeck JL, Dean KM, Ma H, Palmer AE, Jimenez R. Microfluidic flow cytometer for quantifying photobleaching of fluorescent proteins in cells. Anal Chem. 2012 May 01; 84(9):3929-37. PMID: 22424298; PMCID: PMC3341488.
    35. Ma H, Gibson EA, Dittmer PJ, Jimenez R, Palmer AE. High-throughput examination of fluorescence resonance energy transfer-detected metal-ion response in mammalian cells. J Am Chem Soc. 2012 Feb 08; 134(5):2488-91. PMID: 22260720; PMCID: PMC3297669.
    36. Dean KM, Lubbeck JL, Binder JK, Schwall LR, Jimenez R, Palmer AE. Analysis of red-fluorescent proteins provides insight into dark-state conversion and photodegradation. Biophys J. 2011 Aug 17; 101(4):961-9. PMID: 21843488; PMCID: PMC3175071.
    37. Qin Y, Dittmer PJ, Park JG, Jansen KB, Palmer AE. Measuring steady-state and dynamic endoplasmic reticulum and Golgi Zn2+ with genetically encoded sensors. Proc Natl Acad Sci U S A. 2011 May 03; 108(18):7351-6. PMID: 21502528; PMCID: PMC3088641.
    38. Palmer AE, Qin Y, Park JG, McCombs JE. Design and application of genetically encoded biosensors. Trends Biotechnol. 2011 Mar; 29(3):144-52. PMID: 21251723; PMCID: PMC3433949.
    39. Perocchi F, Gohil VM, Girgis HS, Bao XR, McCombs JE, Palmer AE, Mootha VK. MICU1 encodes a mitochondrial EF hand protein required for Ca(2+) uptake. Nature. 2010 Sep 16; 467(7313):291-6. PMID: 20693986; PMCID: PMC2977980.
    40. Ravier MA, Cheng-Xue R, Palmer AE, Henquin JC, Gilon P. Subplasmalemmal Ca(2+) measurements in mouse pancreatic beta cells support the existence of an amplifying effect of glucose on insulin secretion. Diabetologia. 2010 Sep; 53(9):1947-57. PMID: 20461354; PMCID: PMC3297670.
    41. Ong DS, Mu TW, Palmer AE, Kelly JW. Endoplasmic reticulum Ca2+ increases enhance mutant glucocerebrosidase proteostasis. Nat Chem Biol. 2010 Jun; 6(6):424-32. PMID: 20453863; PMCID: PMC2873071.
    42. Palmer AE, Dittmer PJ. SNAP-shots of hydrogen peroxide in cells. Chem Biol. 2010 Apr 23; 17(4):318-9. PMID: 20416502.
      View in: PubMed
    43. McCombs JE, Gibson EA, Palmer AE. Using a genetically targeted sensor to investigate the role of presenilin-1 in ER Ca2+ levels and dynamics. Mol Biosyst. 2010 Sep; 6(9):1640-9. PMID: 20379593; PMCID: PMC3155245.
    44. Van Engelenburg SB, Palmer AE. Imaging type-III secretion reveals dynamics and spatial segregation of Salmonella effectors. Nat Methods. 2010 Apr; 7(4):325-30. PMID: 20228815; PMCID: PMC2862489.
    45. Van Engelenburg SB, Nahreini T, Palmer AE. FACS-based selection of tandem tetracysteine peptides with improved ReAsH brightness in live cells. Chembiochem. 2010 Mar 01; 11(4):489-93. PMID: 20099291; PMCID: PMC3031534.
    46. Palmer AE, Franz KJ. Introduction to "cellular metal homeostasis and trafficking". Chem Rev. 2009 Oct; 109(10):4533-5. PMID: 19778037.
      View in: PubMed
    47. Schafer D, Gibson EA, Salim EA, Palmer AE, Jimenez R, Squier J. Microfluidic cell counter with embedded optical fibers fabricated by femtosecond laser ablation and anodic bonding. Opt Express. 2009 Apr 13; 17(8):6068-73. PMID: 19365429; PMCID: PMC3155247.
    48. Dittmer PJ, Miranda JG, Gorski JA, Palmer AE. Genetically encoded sensors to elucidate spatial distribution of cellular zinc. J Biol Chem. 2009 Jun 12; 284(24):16289-97. PMID: 19363034; PMCID: PMC2713558.
    49. Palmer AE. Expanding the repertoire of fluorescent calcium sensors. ACS Chem Biol. 2009 Mar 20; 4(3):157-9. PMID: 19298092.
      View in: PubMed
    50. McCombs JE, Palmer AE. Measuring calcium dynamics in living cells with genetically encodable calcium indicators. Methods. 2008 Nov; 46(3):152-9. PMID: 18848629; PMCID: PMC2654717.
    51. Wallace DJ, Meyer zum Alten Borgloh S, Astori S, Yang Y, Bausen M, Kügler S, Palmer AE, Tsien RY, Sprengel R, Kerr JN, Denk W, Hasan MT. Single-spike detection in vitro and in vivo with a genetic Ca2+ sensor. Nat Methods. 2008 Sep; 5(9):797-804. PMID: 19160514.
      View in: PubMed
    52. Van Engelenburg SB, Palmer AE. Quantification of real-time Salmonella effector type III secretion kinetics reveals differential secretion rates for SopE2 and SptP. Chem Biol. 2008 Jun; 15(6):619-28. PMID: 18559272; PMCID: PMC3297674.
    53. VanEngelenburg SB, Palmer AE. Fluorescent biosensors of protein function. Curr Opin Chem Biol. 2008 Feb; 12(1):60-5. PMID: 18282482.
      View in: PubMed
    54. Xu C, Xu W, Palmer AE, Reed JC. BI-1 regulates endoplasmic reticulum Ca2+ homeostasis downstream of Bcl-2 family proteins. J Biol Chem. 2008 Apr 25; 283(17):11477-84. PMID: 18299329; PMCID: PMC2431046.
    55. Duman JG, Chen L, Palmer AE, Hille B. Contributions of intracellular compartments to calcium dynamics: implicating an acidic store. Traffic. 2006 Jul; 7(7):859-72. PMID: 16787398.
      View in: PubMed
    56. Palmer AE, Giacomello M, Kortemme T, Hires SA, Lev-Ram V, Baker D, Tsien RY. Ca2+ indicators based on computationally redesigned calmodulin-peptide pairs. Chem Biol. 2006 May; 13(5):521-30. PMID: 16720273.
      View in: PubMed
    57. Palmer AE, Tsien RY. Measuring calcium signaling using genetically targetable fluorescent indicators. Nat Protoc. 2006; 1(3):1057-65. PMID: 17406387.
      View in: PubMed
    58. Quintanar L, Yoon J, Aznar CP, Palmer AE, Andersson KK, Britt RD, Solomon EI. Spectroscopic and electronic structure studies of the trinuclear Cu cluster active site of the multicopper oxidase laccase: nature of its coordination unsaturation. J Am Chem Soc. 2005 Oct 12; 127(40):13832-45. PMID: 16201804.
      View in: PubMed
    59. Palmer AE, Jin C, Reed JC, Tsien RY. Bcl-2-mediated alterations in endoplasmic reticulum Ca2+ analyzed with an improved genetically encoded fluorescent sensor. Proc Natl Acad Sci U S A. 2004 Dec 14; 101(50):17404-9. PMID: 15585581; PMCID: PMC535104.
    60. Shaner NC, Campbell RE, Steinbach PA, Giepmans BN, Palmer AE, Tsien RY. Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein. Nat Biotechnol. 2004 Dec; 22(12):1567-72. PMID: 15558047.
      View in: PubMed
    61. Palmer AE, Szilagyi RK, Cherry JR, Jones A, Xu F, Solomon EI. Spectroscopic characterization of the Leu513His variant of fungal laccase: effect of increased axial ligand interaction on the geometric and electronic structure of the type 1 Cu site. Inorg Chem. 2003 Jun 30; 42(13):4006-17. PMID: 12817956.
      View in: PubMed
    62. Andersson KK, Schmidt PP, Katterle B, Strand KR, Palmer AE, Lee SK, Solomon EI, Gräslund A, Barra AL. Examples of high-frequency EPR studies in bioinorganic chemistry. J Biol Inorg Chem. 2003 Feb; 8(3):235-47. PMID: 12589559.
      View in: PubMed
    63. Campbell RE, Tour O, Palmer AE, Steinbach PA, Baird GS, Zacharias DA, Tsien RY. A monomeric red fluorescent protein. Proc Natl Acad Sci U S A. 2002 Jun 11; 99(12):7877-82. PMID: 12060735; PMCID: PMC122988.
    64. Palmer AE, Quintanar L, Severance S, Wang TP, Kosman DJ, Solomon EI. Spectroscopic characterization and O2 reactivity of the trinuclear Cu cluster of mutants of the multicopper oxidase Fet3p. Biochemistry. 2002 May 21; 41(20):6438-48. PMID: 12009907.
      View in: PubMed
    65. Solomon EI, Chen P, Metz M, Lee SK, Palmer AE. Oxygen Binding, Activation, and Reduction to Water by Copper Proteins. Angew Chem Int Ed Engl. 2001 Dec 17; 40(24):4570-4590. PMID: 12404359.
      View in: PubMed
    66. Palmer AE, Lee SK, Solomon EI. Decay of the peroxide intermediate in laccase: reductive cleavage of the O-O bond. J Am Chem Soc. 2001 Jul 11; 123(27):6591-9. PMID: 11439045.
      View in: PubMed
    67. Machonkin TE, Quintanar L, Palmer AE, Hassett R, Severance S, Kosman DJ, Solomon EI. Spectroscopy and reactivity of the type 1 copper site in Fet3p from Saccharomyces cerevisiae: correlation of structure with reactivity in the multicopper oxidases. J Am Chem Soc. 2001 Jun 13; 123(23):5507-17. PMID: 11389633.
      View in: PubMed
    68. Xu F, Palmer AE, Yaver DS, Berka RM, Gambetta GA, Brown SH, Solomon EI. Targeted mutations in a Trametes villosa laccase. Axial perturbations of the T1 copper. J Biol Chem. 1999 Apr 30; 274(18):12372-5. PMID: 10212209.
      View in: PubMed
    69. Xu F, Berka RM, Wahleithner JA, Nelson BA, Shuster JR, Brown SH, Palmer AE, Solomon EI. Site-directed mutations in fungal laccase: effect on redox potential, activity and pH profile. Biochem J. 1998 Aug 15; 334 ( Pt 1):63-70. PMID: 9693103; PMCID: PMC1219662.
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