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Ram H Nagaraj

TitleProfessor
InstitutionUniversity of Colorado Denver - Anschutz Medical Campus
DepartmentSOM-Ophthalmology

    Collapse Research 
    Collapse research activities and funding
    R29EY009912     (NAGARAJ, RAM H)Sep 1, 1994 - Nov 30, 1997
    NIH/NEI
    MAILLARD REACTION OF ASCORBATE WITH LENS PROTEINS
    Role: Principal Investigator
    R01EY009912     (NAGARAJ, RAM H)Sep 1, 1994 - Mar 31, 2013
    NIH/NEI
    Maillard Reactions and Oxidation in Cataractogenesis
    Role: Principal Investigator
    R21DK068045     (NAGARAJ, RAM H)Jul 1, 2004 - Jun 30, 2007
    NIH/NIDDK
    Novel Pathways of AGE Formation in Diabetes
    Role: Principal Investigator
    R01EY016219     (NAGARAJ, RAM H)Sep 9, 2005 - Aug 31, 2011
    NIH/NEI
    Modifications of Small Heat Shock Proteins in the Lens
    Role: Principal Investigator
    R01EY022061     (NAGARAJ, RAM H)Feb 1, 2013 - Feb 28, 2018
    NIH/NEI
    LENS CAPSULE AND SECONDARY CATARACT
    Role: Principal Investigator
    R01EY023286     (NAGARAJ, RAM H)Sep 1, 2013 - Jul 31, 2017
    NIH/NEI
    Molecular mechanisms of protein crosslinking in the lens
    Role: Principal Investigator

    Collapse Bibliographic 
    Collapse selected publications
    Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Faculty can login to make corrections and additions.
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    1. Nahomi RB, Nagaraj RH. The role of HIF-1a in the TGF-ß2-mediated epithelial-to-mesenchymal transition of human lens epithelial cells. J Cell Biochem. 2018 Apr 25. PMID: 29693273.
      View in: PubMed
    2. Nam MH, Nagaraj RH. Matrix-bound AGEs enhance TGFß2-mediated mesenchymal transition of lens epithelial cells via the noncanonical pathway: implications for secondary cataract formation. Biochem J. 2018 Apr 23; 475(8):1427-1440. PMID: 29588342.
      View in: PubMed
    3. Nahomi RB, Sampathkumar S, Myers AM, Elghazi L, Smith DG, Tang J, Lee CA, Kern TS, Nagaraj RH, Fort PE. The Absence of Indoleamine 2,3-Dioxygenase Inhibits Retinal Capillary Degeneration in Diabetic Mice. Invest Ophthalmol Vis Sci. 2018 Apr 01; 59(5):2042-2053. PMID: 29677366.
      View in: PubMed
    4. Rakete S, Nagaraj RH. UVA Light-mediated Ascorbate Oxidation in Human Lenses. Photochem Photobiol. 2017 Jul; 93(4):1091-1095. PMID: 28084012.
      View in: PubMed
    5. Raghavan CT, Nagaraj RH. Erratum to: AGE-RAGE interaction in the TGFß2-mediated epithelial to mesenchymal transition of human lens epithelial cells. Glycoconj J. 2016 Oct; 33(5):837-838. PMID: 27485830.
      View in: PubMed
    6. Raghavan CT, Nagaraj RH. AGE-RAGE interaction in the TGFß2-mediated epithelial to mesenchymal transition of human lens epithelial cells. Glycoconj J. 2016 08; 33(4):631-43. PMID: 27263094.
      View in: PubMed
    7. Nahomi RB, Pantcheva MB, Nagaraj RH. aB-crystallin is essential for the TGF-ß2-mediated epithelial to mesenchymal transition of lens epithelial cells. Biochem J. 2016 05 15; 473(10):1455-69. PMID: 26987815; PMCID: PMC4928857.
      View in: PubMed, PubMed Central
    8. Rakete S, Nagaraj RH. Identification of Kynoxazine, a Novel Fluorescent Product of the Reaction between 3-Hydroxykynurenine and Erythrulose in the Human Lens, and Its Role in Protein Modification. J Biol Chem. 2016 Apr 29; 291(18):9596-609. PMID: 26941078; PMCID: PMC4850297 [Available on 04/29/17].
      View in: PubMed, PubMed Central
    9. Raghavan CT, Smuda M, Smith AJ, Howell S, Smith DG, Singh A, Gupta P, Glomb MA, Wormstone IM, Nagaraj RH. AGEs in human lens capsule promote the TGFß2-mediated EMT of lens epithelial cells: implications for age-associated fibrosis. Aging Cell. 2016 06; 15(3):465-76. PMID: 26853893.
      View in: PubMed
    10. Panda AK, Nandi SK, Chakraborty A, Nagaraj RH, Biswas A. Differential role of arginine mutations on the structure and functions of a-crystallin. Biochim Biophys Acta. 2016 Jan; 1860(1 Pt B):199-210. PMID: 26080000; PMCID: PMC4914040.
      View in: PubMed, PubMed Central
    11. Smuda M, Henning C, Raghavan CT, Johar K, Vasavada AR, Nagaraj RH, Glomb MA. Comprehensive analysis of maillard protein modifications in human lenses: effect of age and cataract. Biochemistry. 2015 Apr 21; 54(15):2500-7. PMID: 25849437; PMCID: PMC4467907.
      View in: PubMed, PubMed Central
    12. Nagaraj RH, Nahomi RB, Mueller NH, Raghavan CT, Ammar DA, Petrash JM. Therapeutic potential of a-crystallin. Biochim Biophys Acta. 2016 Jan; 1860(1 Pt B):252-7. PMID: 25840354; PMCID: PMC4591086 [Available on 01/01/17].
      View in: PubMed, PubMed Central
    13. Holm T, Raghavan CT, Nahomi R, Nagaraj RH, Kessel L. Effects of photobleaching on selected advanced glycation end products in the human lens. BMC Res Notes. 2015 Jan 16; 8:5. PMID: 25592966; PMCID: PMC4302587.
      View in: PubMed, PubMed Central
    14. Nahomi RB, DiMauro MA, Wang B, Nagaraj RH. Identification of peptides in human Hsp20 and Hsp27 that possess molecular chaperone and anti-apoptotic activities. Biochem J. 2015 Jan 01; 465(1):115-25. PMID: 25332102; PMCID: PMC4425413.
      View in: PubMed, PubMed Central
    15. DiMauro MA, Nandi SK, Raghavan CT, Kar RK, Wang B, Bhunia A, Nagaraj RH, Biswas A. Acetylation of Gly1 and Lys2 promotes aggregation of human ?D-crystallin. Biochemistry. 2014 Nov 25; 53(46):7269-82. PMID: 25393041; PMCID: PMC4245984.
      View in: PubMed, PubMed Central
    16. Linetsky M, Raghavan CT, Johar K, Fan X, Monnier VM, Vasavada AR, Nagaraj RH. UVA light-excited kynurenines oxidize ascorbate and modify lens proteins through the formation of advanced glycation end products: implications for human lens aging and cataract formation. J Biol Chem. 2014 Jun 13; 289(24):17111-23. PMID: 24798334; PMCID: PMC4059152.
      View in: PubMed, PubMed Central
    17. Nahomi RB, Palmer A, Green KM, Fort PE, Nagaraj RH. Pro-inflammatory cytokines downregulate Hsp27 and cause apoptosis of human retinal capillary endothelial cells. Biochim Biophys Acta. 2014 Feb; 1842(2):164-74. PMID: 24252613; PMCID: PMC3905326.
      View in: PubMed, PubMed Central
    18. Nahomi RB, Huang R, Nandi SK, Wang B, Padmanabha S, Santhoshkumar P, Filipek S, Biswas A, Nagaraj RH. Acetylation of lysine 92 improves the chaperone and anti-apoptotic activities of human aB-crystallin. Biochemistry. 2013 Nov 12; 52(45):8126-38. PMID: 24128140; PMCID: PMC3889837.
      View in: PubMed, PubMed Central
    19. Nahomi RB, Wang B, Raghavan CT, Voss O, Doseff AI, Santhoshkumar P, Nagaraj RH. Chaperone peptides of a-crystallin inhibit epithelial cell apoptosis, protein insolubilization, and opacification in experimental cataracts. J Biol Chem. 2013 May 03; 288(18):13022-35. PMID: 23508955; PMCID: PMC3642345.
      View in: PubMed, PubMed Central
    20. Nahomi RB, Oya-Ito T, Nagaraj RH. The combined effect of acetylation and glycation on the chaperone and anti-apoptotic functions of human a-crystallin. Biochim Biophys Acta. 2013 Jan; 1832(1):195-203. PMID: 22982407; PMCID: PMC3518661.
      View in: PubMed, PubMed Central
    21. Nagaraj RH, Nahomi RB, Shanthakumar S, Linetsky M, Padmanabha S, Pasupuleti N, Wang B, Santhoshkumar P, Panda AK, Biswas A. Acetylation of aA-crystallin in the human lens: effects on structure and chaperone function. Biochim Biophys Acta. 2012 Feb; 1822(2):120-9. PMID: 22120592; PMCID: PMC3249504.
      View in: PubMed, PubMed Central
    22. Linetsky M, Kaid Johar SR, Meltretter J, Padmanabha S, Parmar T, Vasavada AR, Pischetsrieder M, Nagaraj RH. Determination of dideoxyosone precursors of AGEs in human lens proteins. Arch Biochem Biophys. 2011 Oct; 514(1-2):16-26. PMID: 21820400; PMCID: PMC3172345.
      View in: PubMed, PubMed Central
    23. Hegde KR, Kowluru RA, Mohr S, Nagaraj RH, Petrash JM. New horizons in research on diabetic complications of the eye: special emphasis on diabetic cataracts and retinopathy. J Ophthalmol. 2010; 2010:979040. PMID: 21049002; PMCID: PMC2964913.
      View in: PubMed, PubMed Central
    24. Rawat A, Nagaraj R. Determinants of membrane association in the SH4 domain of Fyn: roles of N-terminus myristoylation and side-chain thioacylation. Biochim Biophys Acta. 2010 Oct; 1798(10):1854-63. PMID: 20599685.
      View in: PubMed
    25. Mailankot M, Howell S, Nagaraj RH. Kynurenine inhibits fibroblast growth factor 2-mediated expression of crystallins and MIP26 in lens epithelial cells. Biochim Biophys Acta. 2010 Jul-Aug; 1802(7-8):609-20. PMID: 20478381; PMCID: PMC2893274.
      View in: PubMed, PubMed Central
    26. Mailankot M, Nagaraj RH. Induction of indoleamine 2,3-dioxygenase by interferon-gamma in human lens epithelial cells: apoptosis through the formation of 3-hydroxykynurenine. Int J Biochem Cell Biol. 2010 Sep; 42(9):1446-54. PMID: 20435158; PMCID: PMC2910246.
      View in: PubMed, PubMed Central
    27. Gangadhariah MH, Wang B, Linetsky M, Henning C, Spanneberg R, Glomb MA, Nagaraj RH. Hydroimidazolone modification of human alphaA-crystallin: Effect on the chaperone function and protein refolding ability. Biochim Biophys Acta. 2010 Apr; 1802(4):432-41. PMID: 20085807; PMCID: PMC2826572.
      View in: PubMed, PubMed Central
    28. Bonda DJ, Mailankot M, Stone JG, Garrett MR, Staniszewska M, Castellani RJ, Siedlak SL, Zhu X, Lee HG, Perry G, Nagaraj RH, Smith MA. Indoleamine 2,3-dioxygenase and 3-hydroxykynurenine modifications are found in the neuropathology of Alzheimer's disease. Redox Rep. 2010; 15(4):161-8. PMID: 20663292; PMCID: PMC2956440.
      View in: PubMed, PubMed Central
    29. Nagaraj RH, Padmanabha S, Mailankot M, Staniszewska M, Mun LJ, Glomb MA, Linetsky MD. Modulation of advanced glycation endproduct synthesis by kynurenines in human lens proteins. Biochim Biophys Acta. 2010 Apr; 1804(4):829-38. PMID: 20026434; PMCID: PMC2829369.
      View in: PubMed, PubMed Central
    30. Mailankot M, Padmanabha S, Pasupuleti N, Major D, Howell S, Nagaraj RH. Glyoxalase I activity and immunoreactivity in the aging human lens. Biogerontology. 2009 Dec; 10(6):711-20. PMID: 19238574; PMCID: PMC3560407.
      View in: PubMed, PubMed Central
    31. Mailankot M, Staniszewska MM, Butler H, Caprara MH, Howell S, Wang B, Doller C, Reneker LW, Nagaraj RH. Indoleamine 2,3-dioxygenase overexpression causes kynurenine-modification of proteins, fiber cell apoptosis and cataract formation in the mouse lens. Lab Invest. 2009 May; 89(5):498-512. PMID: 19308046; PMCID: PMC2722445.
      View in: PubMed, PubMed Central
    32. Guo ZJ, Niu HX, Hou FF, Zhang L, Fu N, Nagai R, Lu X, Chen BH, Shan YX, Tian JW, Nagaraj RH, Xie D, Zhang X. Advanced oxidation protein products activate vascular endothelial cells via a RAGE-mediated signaling pathway. Antioxid Redox Signal. 2008 Oct; 10(10):1699-712. PMID: 18576917.
      View in: PubMed
    33. Mailankot M, Smith D, Howell S, Wang B, Jacobberger JW, Stefan T, Nagaraj RH. Cell cycle arrest by kynurenine in lens epithelial cells. Invest Ophthalmol Vis Sci. 2008 Dec; 49(12):5466-75. PMID: 18676626; PMCID: PMC2610264.
      View in: PubMed, PubMed Central
    34. Nagaraj RH, Biswas A, Miller A, Oya-Ito T, Bhat M. The other side of the Maillard reaction. Ann N Y Acad Sci. 2008 Apr; 1126:107-12. PMID: 18448802.
      View in: PubMed
    35. Biswas A, Wang B, Miyagi M, Nagaraj RH. Effect of methylglyoxal modification on stress-induced aggregation of client proteins and their chaperoning by human alphaA-crystallin. Biochem J. 2008 Feb 01; 409(3):771-7. PMID: 17941823.
      View in: PubMed
    36. Staniszewska M, Nagaraj RH. Detection of kynurenine modifications in proteins using a monoclonal antibody. J Immunol Methods. 2007 Jul 31; 324(1-2):63-73. PMID: 17574268.
      View in: PubMed
    37. Puttaiah S, Biswas A, Staniszewska M, Nagaraj RH. Methylglyoxal inhibits glycation-mediated loss in chaperone function and synthesis of pentosidine in alpha-crystallin. Exp Eye Res. 2007 May; 84(5):914-21. PMID: 17368444.
      View in: PubMed
    38. Padival S, Nagaraj RH. Pyridoxamine inhibits maillard reactions in diabetic rat lenses. Ophthalmic Res. 2006; 38(5):294-302. PMID: 16974131.
      View in: PubMed
    39. Oya-Ito T, Liu BF, Nagaraj RH. Effect of methylglyoxal modification and phosphorylation on the chaperone and anti-apoptotic properties of heat shock protein 27. J Cell Biochem. 2006 Sep 01; 99(1):279-91. PMID: 16615138.
      View in: PubMed
    40. Harishchandran A, Nagaraj R. Interaction of a pseudosubstrate peptide of protein kinase C and its myristoylated form with lipid vesicles: only the myristoylated form translocates into the lipid bilayer. Biochim Biophys Acta. 2005 Jul 30; 1713(2):73-82. PMID: 15990084.
      View in: PubMed
    41. Staniszewska MM, Nagaraj RH. 3-hydroxykynurenine-mediated modification of human lens proteins: structure determination of a major modification using a monoclonal antibody. J Biol Chem. 2005 Jun 10; 280(23):22154-64. PMID: 15817458.
      View in: PubMed
    42. Nagaraj RH, Oya-Ito T, Padayatti PS, Kumar R, Mehta S, West K, Levison B, Sun J, Crabb JW, Padival AK. Enhancement of chaperone function of alpha-crystallin by methylglyoxal modification. Biochemistry. 2003 Sep 16; 42(36):10746-55. PMID: 12962499.
      View in: PubMed
    43. Kessel L, Kalinin S, Nagaraj RH, Larsen M, Johansson LB. Time-resolved and steady-state fluorescence spectroscopic studies of the human lens with comparison to argpyrimidine, pentosidine and 3-OH-kynurenine. Photochem Photobiol. 2002 Nov; 76(5):549-54. PMID: 12462652.
      View in: PubMed
    44. Nagaraj RH, Sarkar P, Mally A, Biemel KM, Lederer MO, Padayatti PS. Effect of pyridoxamine on chemical modification of proteins by carbonyls in diabetic rats: characterization of a major product from the reaction of pyridoxamine and methylglyoxal. Arch Biochem Biophys. 2002 Jun 01; 402(1):110-9. PMID: 12051689.
      View in: PubMed
    45. Sady C, Jiang CL, Chellan P, Madhun Z, Duve Y, Glomb MA, Nagaraj RH. Maillard reactions by alpha-oxoaldehydes: detection of glyoxal-modified proteins. Biochim Biophys Acta. 2000 Sep 29; 1481(2):255-64. PMID: 11018716.
      View in: PubMed
    46. Shamsi FA, Sharkey E, Creighton D, Nagaraj RH. Maillard reactions in lens proteins: methylglyoxal-mediated modifications in the rat lens. Exp Eye Res. 2000 Mar; 70(3):369-80. PMID: 10712823.
      View in: PubMed
    47. Nagaraj RH, Shamsi FA, Huber B, Pischetsrieder M. Immunochemical detection of oxalate monoalkylamide, an ascorbate-derived Maillard reaction product in the human lens. FEBS Lett. 1999 Jun 25; 453(3):327-30. PMID: 10405169.
      View in: PubMed
    48. Shamsi FA, Lin K, Sady C, Nagaraj RH. Methylglyoxal-derived modifications in lens aging and cataract formation. Invest Ophthalmol Vis Sci. 1998 Nov; 39(12):2355-64. PMID: 9804144.
      View in: PubMed
    49. Shipanova IN, Glomb MA, Nagaraj RH. Protein modification by methylglyoxal: chemical nature and synthetic mechanism of a major fluorescent adduct. Arch Biochem Biophys. 1997 Aug 01; 344(1):29-36. PMID: 9244378.
      View in: PubMed
    50. Nagaraj RH, Kern TS, Sell DR, Fogarty J, Engerman RL, Monnier VM. Evidence of a glycemic threshold for the formation of pentosidine in diabetic dog lens but not in collagen. Diabetes. 1996 May; 45(5):587-94. PMID: 8621008.
      View in: PubMed
    51. Nagaraj RH, Sady C. The presence of a glucose-derived Maillard reaction product in the human lens. FEBS Lett. 1996 Mar 18; 382(3):234-8. PMID: 8605976.
      View in: PubMed
    52. Monnier VM, Nagaraj RH, Portero-Otin M, Glomb M, Elgawish AH, Sell DR, Friedlander MA. Structure of advanced Maillard reaction products and their pathological role. Nephrol Dial Transplant. 1996; 11 Suppl 5:20-6. PMID: 9044302.
      View in: PubMed
    53. Nagaraj RH, Monnier VM. Protein modification by the degradation products of ascorbate: formation of a novel pyrrole from the Maillard reaction of L-threose with proteins. Biochim Biophys Acta. 1995 Nov 15; 1253(1):75-84. PMID: 7492603.
      View in: PubMed
    54. Portero-Otin M, Nagaraj RH, Monnier VM. Chromatographic evidence for pyrraline formation during protein glycation in vitro and in vivo. Biochim Biophys Acta. 1995 Feb 22; 1247(1):74-80. PMID: 7873594.
      View in: PubMed
    55. Nagaraj RH, Monnier VM. Isolation and characterization of a blue fluorophore from human eye lens crystallins: in vitro formation from Maillard reaction with ascorbate and ribose. Biochim Biophys Acta. 1992 Mar 05; 1116(1):34-42. PMID: 1540622.
      View in: PubMed
    56. Nagaraj RH, Sell DR, Prabhakaram M, Ortwerth BJ, Monnier VM. High correlation between pentosidine protein crosslinks and pigmentation implicates ascorbate oxidation in human lens senescence and cataractogenesis. Proc Natl Acad Sci U S A. 1991 Nov 15; 88(22):10257-61. PMID: 1946446; PMCID: PMC52907.
      View in: PubMed, PubMed Central
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