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Anastacia Marie Garcia

TitleAsst Professor
InstitutionUniversity of Colorado Denver - Anschutz Medical Campus
ORCID ORCID Icon0000-0003-2431-2212 Additional info

    Collapse Biography 
    Collapse education and training
    University of Texas Arlington, Arlington, TXBSc05/2009Microbiology
    University of North Texas, Denton, TXPhD08/2015Molecular Biology/Genetics
    Collapse awards and honors
    2022Early Career Investigator Award Finalist, American Heart Association, Young Hearts
    2021 - 2023Loan Repayment Program Award (second renewal), NIH/NHLBI
    2021Outstanding Women in STEM (WiSTEM) Mentorship Award Nominee, CU Anschutz
    2017 - 2019Loan Repayment Program Award, NIH/NHLBI
    2019 - 2021Loan Repayment Program Award (renewal), NIH/NHLBI

    Collapse Overview 
    Collapse overview
    The overall focus of my current research program is to better understand the unique adaptations governing pathological cardiac remodeling and the progression to heart failure in pediatric patients with complex congenital heart disease (CHD), including hypoplastic left heart syndrome (HLHS) and other single ventricle defects. The ultimate goal of my research is to develop the critical knowledgebase and infrastructure necessary to identify efficacious therapies for improving outcomes in this vulnerable group.

    Through our publications we have demonstrated that the heart of patients with single ventricle defects are typified by unique molecular, genetic, and functional changes when compared to the normal heart. By using human cardiac tissue and blood samples in combination with cell and animal models, we have evaluated both the basic biology of complex CHD and interventions that have the potential to address the decline in myocardial function. Currently, a major focus of the lab is to elucidate the molecular mechanisms involved in the modulation of cardiac energy metabolism in single ventricle heart disease. This work will be important for the identification and development of drug therapies that improve cardiac function and enhance transplant-free survival in this population. Additionally, we are actively investigating the use of peripheral blood mononuclear cells (PBMCs) as noninvasive molecular biomarkers in four distinct but complimentary capacities: 1) as a surrogate of the myocardial metabolic environment and presence of HF, 2) as a readout of inflammatory and immune remodeling, 3) for risk stratification and prediction of clinical outcomes, and 4) as a platform to evaluate therapeutic efficacy of novel drug targets to treat complex CHD pathology.

    Collapse Research 
    Collapse research activities and funding
    R01HL171600     (GARCIA, ANASTACIA M)Jun 1, 2024 - Apr 30, 2029
    Glycosphingolipid-Mediated Cardiomyocyte and Immune Cell Dysfunction in Hypoplastic Left Heart Syndrome
    Role: Principal Investigator

    R01HL156670     (MIYAMOTO, SHELLEY DEANNE)Mar 15, 2021 - Jan 31, 2026
    Targeting Mitochondria in Single Ventricle Heart Disease
    Role: Investigator Research Supplement Recipient

    Single Ventricle Research Fund Award     (Anastacia Garcia)Jan 1, 2021 - Dec 31, 2023
    Additional Ventures Foundation
    Peripheral Mononuclear Cells as Biomarkers for Myocardial Mitochondrial Function in Single Ventricle Congenital Heart Disease Patients
    Role Description: The goal of the award is to evaluate mitochondrial function, transcriptomic, metabolomic, and lipidomic alterations, and reactive oxygen species in circulating peripheral blood mononuclear cells in pediatric single ventricle heart disease patients. Completion of her proposal will provide new insights into metabolic remodeling in the single ventricle population and has the potential to identify a novel non-invasive molecular biomarker of myocardial metabolic function and progression to heart failure in this vulnerable group.
    Role: PI

    SRP Award     (Anastacia Garcia)Nov 1, 2020 - Dec 31, 2021
    Defining the Transcriptional Landscape in the Systemic Right Ventricle
    Role Description: The purpose of this small research project is to evaluate mRNA and lncRNA expression in human cardiac tissue, and to identify mRNA-lncRNA regulatory networks with potential roles in the progression to heart failure in the pediatric single ventricle congenital heart disease population relative to healthy age-matched non-failing controls.
    Role: PI

    25M9264 NORC Pilot     (Anastacia Garcia)Aug 1, 2020 - Jul 30, 2022
    Mechanistic Investigations of PDE5-Inhibitor Improved Myocardial Energetics in Congenital Heart Disease
    Role Description: The purpose of this pilot project is to (1) determine the PDE5-mediated changes in carnitine palmitoyltransferase activity and the metabolic lipid profile in the SV failing heart, and (2) to investigate the PDE5-medated changes in mitochondrial lipid oxidation in the SV failing heart.

    UL1TR002535 CMH-Pilot     (Anastacia Garcia)May 1, 2020 - Apr 30, 2021
    NIH/NCATS Colorado CTSI
    Peripheral Mononuclear Cells as Biomarkers for Myocardial Mitochondrial Function in Single Ventricle Congenital Heart Disease Patients
    Role Description: The purpose of this pilot project is to evaluate mitochondrial bioenergetics and reactive oxygen species, and the metabolic signature in circulating PBMCs from pediatric SV patients compared to normal non-failing controls. These data will provide new insights into SV heart failure and has the potential to identify novel biomarkers of disease progression in this population.
    Role: PI

    KL2-TR002534     (Anastacia Garcia)Dec 1, 2019 - Nov 30, 2021
    NIH/NCATS Colorado CTSI
    Metabolic Remodeling in the Failing Pediatric Right Ventricle
    Role Description: The purpose of this project is to determine changes in mitochondrial bioenergetics in explanted pediatric heart tissue and assess the impact of PDE5 inhibition in human SV myocardium and in primary cardiomyocytes treated with SV patient serum. The PDE5-mediated metabolic consequences of sphingolipid remodeling in vitro and in vivo will also be assessed.
    Role: PI

    Collapse Bibliographic 
    Collapse selected publications
    Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Faculty can login to make corrections and additions.
    List All   |   Timeline
    1. Pires Da Silva J, Padilla PA, Garcia AM. Editorial: The intersection of gene regulation and metabolism in cardiovascular disease. Front Genet. 2023; 14:1253690. PMID: 37701624.
      View in: PubMed
    2. Garcia AM, Toni LS, Miyano CA, Sparagna GC, Jonscher R, Phillips EK, Karimpour-Fard A, Chapman HL, Baybayon-Grandgeorge AN, Pietra AE, Selner E, Chatfield KC, Stauffer BL, Sucharov CC, Miyamoto SD. Cardiac Transcriptome Remodeling and?Impaired Bioenergetics in Single-Ventricle Congenital Heart Disease. JACC Basic Transl Sci. 2023 Mar; 8(3):258-279. PMID: 37034285.
      View in: PubMed
    3. Sparagna GC, Jonscher RL, Shuff SR, Phillips EK, Wilson CE, Woulfe KC, Garcia AM, Stauffer BL, Chatfield KC. Linoleate-Enrichment of Mitochondrial Cardiolipin Molecular Species Is Developmentally Regulated and a Determinant of Metabolic Phenotype. Biology (Basel). 2022 Dec 24; 12(1). PMID: 36671725.
      View in: PubMed
    4. Tsai CW, Rodriguez MX, Van Keuren AM, Phillips CB, Shushunov HM, Lee JE, Garcia AM, Ambardekar AV, Cleveland JC, Reisz JA, Proenza C, Chatfield KC, Tsai MF. Mechanisms and significance of tissue-specific MICU regulation of the mitochondrial calcium uniporter complex. Mol Cell. 2022 10 06; 82(19):3661-3676.e8. PMID: 36206740.
      View in: PubMed
    5. Baybayon-Grandgeorge AN, Pietra AE, Miyamoto SD, Garcia AM. Medical Therapies for Heart Failure in Hypoplastic Left Heart Syndrome. J Cardiovasc Dev Dis. 2022 May 12; 9(5). PMID: 35621863.
      View in: PubMed
    6. Pires da Silva J, Pietra AE, Baybayon-Grandgeorge AN, and Garcia AM. Serum Metabolic Profiling Identifies Key Differences between Patients with Single-Ventricle Heart Disease and Healthy Controls. Int. J. Transl. Med. 2022; 1(2):78-96.
    7. Jeffrey DA, Pires Da Silva J, Garcia AM, Jiang X, Karimpour-Fard A, Toni LS, Lanzicher T, Pe?a B, Miyano CA, Nunley K, Korst A, Sbaizero O, Taylor MR, Miyamoto SD, Stauffer BL, Sucharov CC. Serum circulating proteins from pediatric patients with dilated cardiomyopathy cause pathologic remodeling and cardiomyocyte stiffness. JCI Insight. 2021 10 08; 6(19). PMID: 34383712.
      View in: PubMed
    8. Wall JB, Garcia AM, Jacobsen RM, Miyamoto SD. Important Considerations in Pediatric Heart Failure. Curr Cardiol Rep. 2020 09 10; 22(11):141. PMID: 32910348.
      View in: PubMed
    9. Rubinstein J, Woo JG, Garcia AM, Alsaied T, Li J, Lunde PK, Moore RA, Laasmaa M, Sammons A, Mays WA, Miyamoto SD, Louch WE, Veldtman GR. Probenecid Improves Cardiac Function in Subjects with a Fontan Circulation and Augments Cardiomyocyte Calcium Homeostasis. Pediatr Cardiol. 2020 Dec; 41(8):1675-1688. PMID: 32770262.
      View in: PubMed
    10. Ohlstrom D, Hernandez-Lagunas L, Garcia AM, Allawzi A, Karimpour-Fard A, Sucharov CC, Nozik-Grayck E. MicroRNA regulation postbleomycin due to the R213G extracellular superoxide dismutase variant is predicted to suppress inflammatory and immune pathways. Physiol Genomics. 2020 06 01; 52(6):245-254. PMID: 32421439.
      View in: PubMed
    11. Garcia AM, Beatty JT, Nakano SJ. Heart failure in single right ventricle congenital heart disease: physiological and molecular considerations. Am J Physiol Heart Circ Physiol. 2020 04 01; 318(4):H947-H965. PMID: 32108525.
      View in: PubMed
    12. Garcia AM, McPhaul JC, Sparagna GC, Jeffrey DA, Jonscher R, Patel SS, Sucharov CC, Stauffer BL, Miyamoto SD, Chatfield KC. Alteration of cardiolipin biosynthesis and remodeling in single right ventricle congenital heart disease. Am J Physiol Heart Circ Physiol. 2020 04 01; 318(4):H787-H800. PMID: 32056460.
      View in: PubMed
    13. Nakano SJ, Walker JS, Walker LA, Li X, Du Y, Miyamoto SD, Sucharov CC, Garcia AM, Mitchell MB, Ambardekar AV, Stauffer BL. Increased myocyte calcium sensitivity in end-stage pediatric dilated cardiomyopathy. Am J Physiol Heart Circ Physiol. 2019 12 01; 317(6):H1221-H1230. PMID: 31625780.
      View in: PubMed
    14. Garcia AM, Nakano SJ, Karimpour-Fard A, Nunley K, Blain-Nelson P, Stafford NM, Stauffer BL, Sucharov CC, Miyamoto SD. Phosphodiesterase-5 Is Elevated in Failing Single Ventricle Myocardium and Affects Cardiomyocyte Remodeling In Vitro. Circ Heart Fail. 2018 09; 11(9):e004571. PMID: 30354365.
      View in: PubMed
    15. Garcia AM, Allawzi A, Tatman P, Hernandez-Lagunas L, Swain K, Mouradian G, Bowler R, Karimpour-Fard A, Sucharov CC, Nozik-Grayck E. R213G polymorphism in SOD3 protects against bleomycin-induced inflammation and attenuates induction of proinflammatory pathways. Physiol Genomics. 2018 09 01; 50(9):807-816. PMID: 30004839.
      View in: PubMed
    16. Toni LS, Garcia AM, Jeffrey DA, Jiang X, Stauffer BL, Miyamoto SD, Sucharov CC. Optimization of phenol-chloroform RNA extraction. MethodsX. 2018; 5:599-608. PMID: 29984193.
      View in: PubMed
    17. Nakano SJ, Siomos AK, Garcia AM, Nguyen H, SooHoo M, Galambos C, Nunley K, Stauffer BL, Sucharov CC, Miyamoto SD. Fibrosis-Related Gene Expression in Single Ventricle Heart Disease. J Pediatr. 2017 12; 191:82-90.e2. PMID: 29050751.
      View in: PubMed
    18. Ladage ML, King SD, Burks DJ, Quan DL, Garcia AM, Azad RK, Padilla PA. Glucose or Altered Ceramide Biosynthesis Mediate Oxygen Deprivation Sensitivity Through Novel Pathways Revealed by Transcriptome Analysis in Caenorhabditis elegans. G3 (Bethesda). 2016 10 13; 6(10):3149-3160. PMID: 27507791.
      View in: PubMed
    19. Garcia AM, Ladage ML, Dumesnil DR, Zaman K, Shulaev V, Azad RK, Padilla PA. Glucose induces sensitivity to oxygen deprivation and modulates insulin/IGF-1 signaling and lipid biosynthesis in Caenorhabditis elegans. Genetics. 2015 May; 200(1):167-84. PMID: 25762526.
      View in: PubMed
    20. Padilla PA, Garcia AM, Ladage ML, Toni LS. Caenorhabditis elegans: an old genetic model can learn new epigenetic tricks. Integr Comp Biol. 2014 Jul; 54(1):52-60. PMID: 24861810.
      View in: PubMed
    21. Garcia AM, Ladage ML, Padilla PA. Use of time lapse microscopy to visualize anoxia-induced suspended animation in C. elegans embryos. J Vis Exp. 2012 Dec 03; (70):e4319. PMID: 23242305.
      View in: PubMed
    22. Shi L, Ermis R, Garcia A, Telgenhoff D, Aust D. Degradation of human collagen isoforms by Clostridium collagenase and the effects of degradation products on cell migration. Int Wound J. 2010 Apr; 7(2):87-95. PMID: 20529148.
      View in: PubMed
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