Ligase Chain Reaction
"Ligase Chain Reaction" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus,
MeSH (Medical Subject Headings). Descriptors are arranged in a hierarchical structure,
which enables searching at various levels of specificity.
A DNA amplification technique based upon the ligation of OLIGONUCLEOTIDE PROBES. The probes are designed to exactly match two adjacent sequences of a specific target DNA. The chain reaction is repeated in three steps in the presence of excess probe: (1) heat denaturation of double-stranded DNA, (2) annealing of probes to target DNA, and (3) joining of the probes by thermostable DNA ligase. After the reaction is repeated for 20-30 cycles the production of ligated probe is measured.
| Descriptor ID |
D022521
|
| MeSH Number(s) |
E05.393.620.311
|
| Concept/Terms |
Ligase Chain Reaction- Ligase Chain Reaction
- Chain Reaction, Ligase
- Chain Reactions, Ligase
- Ligase Chain Reactions
- Reaction, Ligase Chain
- Reactions, Ligase Chain
|
Below are MeSH descriptors whose meaning is more general than "Ligase Chain Reaction".
Below are MeSH descriptors whose meaning is more specific than "Ligase Chain Reaction".
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Below are the most recent publications written about "Ligase Chain Reaction" by people in Profiles.
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Wagner TA, Kress CM, Beck I, Techapornroong M, Wittayapraparat P, Tansuphasawasdikul S, Jourdain G, Ngo-Giang-Huong N, Lallemant M, Frenkel LM. Detection of HIV-1 drug resistance in women following administration of a single dose of nevirapine: comparison of plasma RNA to cellular DNA by consensus sequencing and by oligonucleotide ligation assay. J Clin Microbiol. 2010 May; 48(5):1555-61.