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Search Results to John Lute Markley

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Markley, John

One or more keywords matched the following items that are connected to Markley, John

Item Type	Name
Concept	Homeodomain Proteins
Concept	Adaptor Proteins, Signal Transducing
Concept	Membrane Proteins
Concept	DNA-Binding Proteins
Concept	Heat-Shock Proteins
Concept	Neurofilament Proteins
Concept	HSP40 Heat-Shock Proteins
Concept	HSP70 Heat-Shock Proteins
Concept	Escherichia coli Proteins
Concept	Green Fluorescent Proteins
Concept	Bacterial Proteins
Concept	Recombinant Fusion Proteins
Concept	Calcium-Binding Proteins
Concept	Viral Nonstructural Proteins
Concept	Plant Proteins
Concept	Fungal Proteins
Concept	Saccharomyces cerevisiae Proteins
Concept	Mitochondrial Proteins
Concept	Carrier Proteins
Concept	Luminescent Proteins
Concept	Iron-Sulfur Proteins
Concept	Nuclear Proteins
Concept	Recombinant Proteins
Concept	Nonheme Iron Proteins
Concept	Proto-Oncogene Proteins p21(ras)
Concept	Mutant Proteins
Concept	Iron-Binding Proteins
Concept	Arabidopsis Proteins
Concept	Cation Transport Proteins
Concept	Proto-Oncogene Proteins
Concept	Nerve Tissue Proteins
Concept	Tumor Suppressor Proteins
Concept	Viral Proteins
Concept	Intercellular Signaling Peptides and Proteins
Concept	Iron-Regulatory Proteins
Concept	Repressor Proteins
Concept	Xenopus Proteins
Concept	Proteins
Concept	Maltose-Binding Proteins
Academic Article	PACSY, a relational database management system for protein structure and chemical shift analysis.
Academic Article	The AUDANA algorithm for automated protein 3D structure determination from NMR NOE data.
Academic Article	Integrative NMR for biomolecular research.
Academic Article	Solution structure of the 2A protease from a common cold agent, human rhinovirus C2, strain W12.
Academic Article	Broadening the functionality of a J-protein/Hsp70 molecular chaperone system.
Academic Article	NMRmix: A Tool for the Optimization of Compound Mixtures in 1D (1)H NMR Ligand Affinity Screens.
Academic Article	Structure and evolution of the 4-helix bundle domain of Zuotin, a J-domain protein co-chaperone of Hsp70.
Academic Article	Molecular insights into the recognition of N-terminal histone modifications by the BRPF1 bromodomain.
Academic Article	CHESPA/CHESCA-SPARKY: automated NMR data analysis plugins for SPARKY to map protein allostery.
Academic Article	Solution structure of human myeloid-derived growth factor suggests a conserved function in the endoplasmic reticulum.
Academic Article	I-PINE web server: an integrative probabilistic NMR assignment system for proteins.
Academic Article	Backbone resonance assignments and secondary structure of Ebola nucleoprotein 600-739 construct.
Academic Article	Probabilistic validation of protein NMR chemical shift assignments.
Academic Article	PONDEROSA-C/S: client-server based software package for automated protein 3D structure determination.
Academic Article	NMRFAM-SDF: a protein structure determination framework.
Academic Article	Function and solution structure of the Arabidopsis thaliana RALF8 peptide.
Academic Article	ADAPT-NMR Enhancer: complete package for reduced dimensionality in protein NMR spectroscopy.
Academic Article	Construction and performance of an NMR tube with a sample cavity formed within magnetic susceptibility-matched glass.
Academic Article	NMRFAM-SPARKY: enhanced software for biomolecular NMR spectroscopy.
Academic Article	PONDEROSA, an automated 3D-NOESY peak picking program, enables automated protein structure determination.
Academic Article	Mixing apparatus for preparing NMR samples under pressure.
Academic Article	Fast automated protein NMR data collection and assignment by ADAPT-NMR on Bruker spectrometers.
Academic Article	PISA-SPARKY: an interactive SPARKY plugin to analyze oriented solid-state NMR spectra of helical membrane proteins.
Academic Article	PINE-SPARKY: graphical interface for evaluating automated probabilistic peak assignments in protein NMR spectroscopy.
Grant	The BMRB as an evolving resource for biomolecular structure-function research
Grant	Center for Eukaryotic Structural Genomics
Grant	Specialized Center for Eukaryotic Structural Genomics
Grant	NMR Spectroscopy of Iron-Sulfur Proteins
Grant	Partnership for High-Throughput Enabled Biology of the Mitochondrial Proteome
Grant	National Biomedical NMR Resource at Madison
Grant	National Biomedical NMR Resource at Madison
Grant	NMR SPECTROSCOPY IN PROTEIN CHEMISTRY
Grant	Integrated state-of-the-art LC-MS/NMR system for the University of Wisconsin
Grant	WIDE-BORE 9.4 TESLA NMR SPECTROMETER FOR UW-MADISON
Grant	Biological Magnetic Resonance Data Bank
Grant	NMR SPECTROSCOPY IN PROTEIN CHEMISTRY
Grant	Biogenesis of human mitochondrial iron-sulfur proteins
Grant	900 MHZ NMR SPECTROMETER
Grant	NMR Spectrometer System Optimized for Direct 13C or 15N Detection
Grant	CREATION AND ANALYSIS OF ARCHIVAL PROTEIN NMR DATABASE
Academic Article	An automated system designed for large scale NMR data deposition and annotation: application to over 600 assigned chemical shift data entries to the BioMagResBank from the Riken Structural Genomics/Proteomics Initiative internal database.
Academic Article	ADAPT-NMR 3.0: utilization of BEST-type triple-resonance NMR experiments to accelerate the process of data collection and assignment.
Academic Article	Assignment of 1H, 13C and 15N NMR signals from toluene 4-monooxygenase Rieske ferredoxin in its oxidized state.
Academic Article	Solution studies of staphylococcal nuclease H124L. 2. 1H, 13C, and 15N chemical shift assignments for the unligated enzyme and analysis of chemical shift changes that accompany formation of the nuclease-thymidine 3',5'-bisphosphate-calcium ternary complex.
Academic Article	Recommendations for the presentation of NMR structures of proteins and nucleic acids--IUPAC-IUBMB-IUPAB Inter-Union Task Group on the standardization of data bases of protein and nucleic acid structures determined by NMR spectroscopy.
Academic Article	Protein energetic conformational analysis from NMR chemical shifts (PECAN) and its use in determining secondary structural elements.
Academic Article	Probabilistic interaction network of evidence algorithm and its application to complete labeling of peak lists from protein NMR spectroscopy.
Academic Article	Recommendations for the presentation of NMR structures of proteins and nucleic acids. IUPAC-IUBMB-IUPAB Inter-Union Task Group on the Standardization of Data Bases of Protein and Nucleic Acid Structures Determined by NMR Spectroscopy.
Academic Article	Assignment of 1H, 13C and 15N NMR signals in the toluene 4-monooxygenase effector protein.
Academic Article	Probabilistic approach to determining unbiased random-coil carbon-13 chemical shift values from the protein chemical shift database.
Academic Article	Evaluation of an algorithm for the automated sequential assignment of protein backbone resonances: a demonstration of the connectivity tracing assignment tools (CONTRAST) software package.
Academic Article	Human Mitochondrial Ferredoxin 1 (FDX1) and Ferredoxin 2 (FDX2) Both Bind Cysteine Desulfurase and Donate Electrons for Iron-Sulfur Cluster Biosynthesis.
Academic Article	Three-dimensional structure and determinants of stability of the iron-sulfur cluster scaffold protein IscU from Escherichia coli.
Academic Article	BioMagResBank database with sets of experimental NMR constraints corresponding to the structures of over 1400 biomolecules deposited in the Protein Data Bank.
Academic Article	Strategy for the study of paramagnetic proteins with slow electronic relaxation rates by nmr spectroscopy: application to oxidized human [2Fe-2S] ferredoxin.
Academic Article	Publication of nuclear magnetic resonance experimental data with semantic web technology and the application thereof to biomedical research of proteins.
Academic Article	Outcome of the First wwPDB Hybrid/Integrative Methods Task Force Workshop.
Academic Article	Interactions of iron-bound frataxin with ISCU and ferredoxin on the cysteine desulfurase complex leading to Fe-S cluster assembly.
Academic Article	Outcome of the First wwPDB/CCDC/D3R Ligand Validation Workshop.
Academic Article	Structures of proteins of biomedical interest from the Center for Eukaryotic Structural Genomics.
Academic Article	ISCU(M108I) and ISCU(D39V) Differ from Wild-Type ISCU in Their Failure To Form Cysteine Desulfurase Complexes Containing Both Frataxin and Ferredoxin.
Academic Article	Efficient and rapid protein expression and purification of small high disulfide containing sweet protein brazzein in E. coli.
Academic Article	Automated cell-free protein production methods for structural studies.
Academic Article	Tangled web of interactions among proteins involved in iron-sulfur cluster assembly as unraveled by NMR, SAXS, chemical crosslinking, and functional studies.
Academic Article	ISCU interacts with NFU1, and ISCU[4Fe-4S] transfers its Fe-S cluster to NFU1 leading to the production of holo-NFU1.
Academic Article	Crystal structure of At2g03760, a putative steroid sulfotransferase from Arabidopsis thaliana.
Academic Article	Solution structure of the N-domain of Wilson disease protein: distinct nucleotide-binding environment and effects of disease mutations.
Academic Article	Macromolecular structure determination by NMR spectroscopy.
Academic Article	View from Nuclear Magnetic Resonance Spectroscopy.
Academic Article	HIFI-C: a robust and fast method for determining NMR couplings from adaptive 3D to 2D projections.
Academic Article	Lipid binding ridge on loops 2 and 3 of the C2A domain of synaptotagmin I as revealed by NMR spectroscopy.
Academic Article	Recommendations for the presentation of NMR structures of proteins and nucleic acids.
Academic Article	Solution structure of a late embryogenesis abundant protein (LEA14) from Arabidopsis thaliana, a cellular stress-related protein.
Academic Article	Practical model fitting approaches to the direct extraction of NMR parameters simultaneously from all dimensions of multidimensional NMR spectra.
Academic Article	Conserved Lipid and Small-Molecule Modulation of COQ8 Reveals Regulation of the Ancient Kinase-like UbiB Family.
Academic Article	Correlation of the sweetness of variants of the protein brazzein with patterns of hydrogen bonds detected by NMR spectroscopy.
Academic Article	Use of NMR saturation transfer difference spectroscopy to study ligand binding to membrane proteins.
Academic Article	Interactions between the human sweet-sensing T1R2-T1R3 receptor and sweeteners detected by saturation transfer difference NMR spectroscopy.
Academic Article	Solution structure of the toluene 4-monooxygenase effector protein (T4moD).
Academic Article	Disordered form of the scaffold protein IscU is the substrate for iron-sulfur cluster assembly on cysteine desulfurase.
Academic Article	(13)C[(13)C] 2D NMR: a novel strategy for the study of paramagnetic proteins with slow electronic relaxation rates.
Academic Article	Uncovering a membrane-distal conformation of KRAS available to recruit RAF to the plasma membrane.
Academic Article	Monkey electrophysiological and human psychophysical responses to mutants of the sweet protein brazzein: delineating brazzein sweetness.
Academic Article	Protein inhibitors of serine proteinases: role of backbone structure and dynamics in controlling the hydrolysis constant.
Academic Article	PDB-Dev: a Prototype System for Depositing Integrative/Hybrid Structural Models.
Academic Article	Linear analysis of carbon-13 chemical shift differences and its application to the detection and correction of errors in referencing and spin system identifications.
Academic Article	Crystal structure of the protein from gene At3g17210 of Arabidopsis thaliana.
Academic Article	Probabilistic identification of saccharide moieties in biomolecules and their protein complexes.
Academic Article	Metamorphic protein IscU changes conformation by cis-trans isomerizations of two peptidyl-prolyl peptide bonds.
Academic Article	Differences in the structure and dynamics of the apo- and palmitate-ligated forms of Aedes aegypti sterol carrier protein 2 (AeSCP-2).
Academic Article	Announcing mandatory submission of PDBx/mmCIF format files for crystallographic depositions to the Protein Data Bank (PDB).
Academic Article	Structure-function relationships of brazzein variants with altered interactions with the human sweet taste receptor.
Academic Article	Solution structure of YggX: a prokaryotic protein involved in Fe(II) trafficking.
Academic Article	NMR structure of the mengovirus Leader protein zinc-finger domain.
Academic Article	Three-dimensional structure of the water-insoluble protein crambin in dodecylphosphocholine micelles and its minimal solvent-exposed surface.
Academic Article	NMR as a Tool to Investigate the Processes of Mitochondrial and Cytosolic Iron-Sulfur Cluster Biosynthesis.
Academic Article	Auto-induction medium containing glyphosate for high-level incorporation of unusual aromatic amino acids into proteins.
Academic Article	Structural Insights into the Recognition of Mono- and Diacetylated Histones by the ATAD2B Bromodomain.
Academic Article	Preparation of isotopically labeled spinach acyl-acyl carrier protein for NMR structural studies.
Academic Article	Solution structure of a cyanobacterial phytochrome GAF domain in the red-light-absorbing ground state.
Academic Article	Federating Structural Models and Data: Outcomes from A Workshop on Archiving Integrative Structures.
Academic Article	Temperature-dependent conformational change affecting Tyr11 and sweetness loops of brazzein.
Academic Article	Structure and Function of the PriC DNA Replication Restart Protein.
Academic Article	The Complex Energy Landscape of the Protein IscU.
Academic Article	Metamorphic protein IscU alternates conformations in the course of its role as the scaffold protein for iron-sulfur cluster biosynthesis and delivery.
Academic Article	Current Solution NMR Techniques for Structure-Function Studies of Proteins and RNA Molecules.
Academic Article	Oligomerization of the EK18 mutant of the trp repressor of Escherichia coli as observed by NMR spectroscopy.
Academic Article	A graphical approach to tracking and reporting target status in structural genomics.
Academic Article	Monomeric solution structure of the prototypical 'C' chemokine lymphotactin.
Academic Article	Efficient stable isotope labeling and purification of vitamin D receptor from inclusion bodies.
Academic Article	Structural/Functional Properties of Human NFU1, an Intermediate [4Fe-4S] Carrier in Human Mitochondrial Iron-Sulfur Cluster Biogenesis.
Academic Article	Structural characterization of Hsp12, the heat shock protein from Saccharomyces cerevisiae, in aqueous solution where it is intrinsically disordered and in detergent micelles where it is locally a-helical.
Academic Article	Mitochondrial Cysteine Desulfurase and ISD11 Coexpressed in Escherichia coli Yield Complex Containing Acyl Carrier Protein.
Academic Article	Coordination of Di-Acetylated Histone Ligands by the ATAD2 Bromodomain.
Academic Article	Structural basis for RNA recognition by a type II poly(A)-binding protein.
Academic Article	Cell-free protein synthesis for functional and structural studies.
Academic Article	The specialized Hsp70 (HscA) interdomain linker binds to its nucleotide-binding domain and stimulates ATP hydrolysis in both cis and trans configurations.
Academic Article	MolProbity for the masses-of data.
Academic Article	Rieske protein from Thermus thermophilus: 15N NMR titration study demonstrates the role of iron-ligated histidines in the pH dependence of the reduction potential.
Academic Article	Mechanism of Histone H3K4me3 Recognition by the Plant Homeodomain of Inhibitor of Growth 3.
Academic Article	Expression platforms for producing eukaryotic proteins: a comparison of E. coli cell-based and wheat germ cell-free synthesis, affinity and solubility tags, and cloning strategies.
Academic Article	High-resolution iterative frequency identification for NMR as a general strategy for multidimensional data collection.
Academic Article	Wheat germ cell-free platform for eukaryotic protein production.
Academic Article	Brazzein, a small, sweet protein: effects of mutations on its structure, dynamics and functional properties.
Academic Article	Solution structure of At3g04780.1-des15, an Arabidopsis thaliana ortholog of the C-terminal domain of human thioredoxin-like protein.
Academic Article	Auto-induction medium for the production of [U-15N]- and [U-13C, U-15N]-labeled proteins for NMR screening and structure determination.
Academic Article	Carbonyl carbon label selective (CCLS) 1H-15N HSQC experiment for improved detection of backbone 13C-15N cross peaks in larger proteins.
Academic Article	Hydrogen exchange during cell-free incorporation of deuterated amino acids and an approach to its inhibition.
Academic Article	Small-scale, semi-automated purification of eukaryotic proteins for structure determination.
Academic Article	Ligand-specific structural changes in the vitamin D receptor in solution.
Academic Article	Structural basis for the catalytic mechanism of mammalian 25-kDa thiamine triphosphatase.
Academic Article	Direct NMR detection of the binding of functional ligands to the human sweet receptor, a heterodimeric family 3 GPCR.
Academic Article	Efficient production of recombinant brazzein, a small, heat-stable, sweet-tasting protein of plant origin.
Academic Article	BioMagResBank (BMRB) as a Resource for Structural Biology.
Academic Article	The Center for Eukaryotic Structural Genomics.
Academic Article	The Protein Data Bank Archive.
Academic Article	pH-induced conformational change of IscU at low pH correlates with protonation/deprotonation of two conserved histidine residues.
Academic Article	Functional recombinant rabbit muscle phosphoglucomutase from Escherichia coli.
Academic Article	Three hydrophobic amino acids in Escherichia coli HscB make the greatest contribution to the stability of the HscB-IscU complex.
Academic Article	Probing Protein-Protein Interactions Using Asymmetric Labeling and Carbonyl-Carbon Selective Heteronuclear NMR Spectroscopy.
Academic Article	Probabilistic Identification of Spin Systems and their Assignments including Coil-Helix Inference as Output (PISTACHIO).
Academic Article	Dynamical Structures of Hsp70 and Hsp70-Hsp40 Complexes.
Academic Article	Architectural Features of Human Mitochondrial Cysteine Desulfurase Complexes from Crosslinking Mass Spectrometry and Small-Angle X-Ray Scattering.
Academic Article	Interactions between metal-binding domains modulate intracellular targeting of Cu(I)-ATPase ATP7B, as revealed by nanobody binding.
Academic Article	Key amino acid residues involved in multi-point binding interactions between brazzein, a sweet protein, and the T1R2-T1R3 human sweet receptor.
Academic Article	BioMagResBank.
Academic Article	One-sample approach to determine the relative orientations of proteins in ternary and binary complexes from residual dipolar coupling measurements.
Academic Article	Sweetness determinant sites of brazzein, a small, heat-stable, sweet-tasting protein.
Academic Article	Solution structure of a small protein containing a fluorinated side chain in the core.
Academic Article	The second finger of Urbs1 is required for iron-mediated repression of sid1 in Ustilago maydis.
Academic Article	Nearest-neighbor effects on backbone alpha and beta carbon chemical shifts in proteins.
Academic Article	Production in two-liter beverage bottles of proteins for NMR structure determination labeled with either 15N- or 13C-15N.
Academic Article	Protein Data Bank (PDB): The Single Global Macromolecular Structure Archive.
Academic Article	Biophysical characterization of a-synuclein and its controversial structure.
Academic Article	Structure of the insect cytokine peptide plasmatocyte-spreading peptide 1 from Pseudoplusia includens.
Academic Article	Electron-nuclear interactions in two prototypical [2Fe-2S] proteins: selective (chiral) deuteration and analysis of (1)H and (2)H NMR signals from the alpha and beta hydrogens of cysteinyl residues that ligate the iron in the active sites of human ferredoxin and Anabaena 7120 vegetative ferredoxin.

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