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Search Results to Bradley J Stith

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One or more keywords matched the following properties of Stith, Bradley

overview We have suggested a new step in fertilization involving Src tyrosine kinase activation by a lipid called phosphatidic acid (PA). Large-scale HPLC/ELSD quantification of lipids at fertilization (Petcoff et al. J Lipid Res 2365-78, 2008) suggests that production of PA through the action of phospholipase D has a role in fertilization. In an action that requires intact membrane rafts, PA activates Src tyrosine kinase, which then activates phospholipase C (PLC?) to produce IP3 and subsequent intracellular calcium release to induce fertilization in Xenopus laevis (Stith, Developmental Biology 401(2):188–205; Bates et al., Developmental Biology 386:165-180, 2014). This work suggests a new path to cancer as PA is elevated and Src is hyperactivated in many forms of cancer. Based on our lipid-Src binding work, studies by Stuart McLaughlin (SUNY Stony Brook) and Miguel Pons (Univ of Barcelona), anionic lipids may bind to the SH4 domain, the ULBR and SH3 domains of Src to “loosen” the tight configuration of Src to lead to its activation. Support: NIH (HD065661) and the University of Colorado Denver Undergraduate Research Opportunity Program

One or more keywords matched the following items that are connected to Stith, Bradley

Item TypeName
Academic Article Sperm increase inositol 1,4,5-trisphosphate mass in Xenopus laevis eggs preinjected with calcium buffers or heparin.
Academic Article Inositol 1,4,5-trisphosphate mass changes from fertilization through first cleavage in Xenopus laevis.
Academic Article The effect of insulin on intracellular ph and ribosomal protein S6 phosphorylation in oocytes of Xenopus laevis.
Academic Article sn-1,2-diacylglycerol and choline increase after fertilization in Xenopus laevis.
Academic Article Protein kinase C initially inhibits the induction of meiotic cell division in Xenopus oocytes.
Academic Article Quantification of major classes of Xenopus phospholipids by high performance liquid chromatography with evaporative light scattering detection.
Academic Article Nongenomic action of progesterone: activation of Xenopus oocyte phospholipase C through a plasma membrane-associated tyrosine kinase.
Academic Article Quantification of phosphatidic acid and lysophosphatidic acid by HPLC with evaporative light-scattering detection.
Academic Article Increased intracellular pH is not necessary for ribosomal protein s6 phosphorylation, increased protein synthesis, or germinal vesicle breakdown in Xenopus oocytes.
Academic Article Insulin-like growth factor 1, insulin, and progesterone induce early and late increases in Xenopus oocyte sn-1,2-diacylglycerol levels before meiotic cell division.
Academic Article Metformin (Glucophage) inhibits tyrosine phosphatase activity to stimulate the insulin receptor tyrosine kinase.
Academic Article Insulin and progesterone increase 32PO4-labeling of phospholipids and inositol 1,4,5-trisphosphate mass in Xenopus oocytes.
Academic Article The antidiabetic drug metformin elevates receptor tyrosine kinase activity and inositol 1,4,5-trisphosphate mass in Xenopus oocytes.
Academic Article Detergent-free domain isolated from Xenopus egg plasma membrane with properties similar to those of detergent-resistant membranes.
Academic Article Signal transduction pathways leading to Ca2+ release in a vertebrate model system: lessons from Xenopus eggs.
Academic Article Lipid levels in sperm, eggs, and during fertilization in Xenopus laevis.
Academic Article Microinjection of inositol 1,2-(cyclic)-4,5-trisphosphate, inositol 1,3,4,5-tetrakisphosphate, and inositol 1,4,5-trisphosphate into intact Xenopus oocytes can induce membrane currents independent of extracellular calcium.
Academic Article Induction of meiotic maturation in Xenopus oocytes by 12-O-tetradecanoylphorbol 13-acetate.
Academic Article Increased phosphorylation of ribosomal protein S6 following microinjection of insulin receptor-kinase into Xenopus oocytes.
Concept Xenopus laevis
Concept Xenopus
Concept Xenopus Proteins
Academic Article Activation of Src and release of intracellular calcium by phosphatidic acid during Xenopus laevis fertilization.
Academic Article Phospholipase C and D regulation of Src, calcium release and membrane fusion during Xenopus laevis development
Academic Article Phospholipase C and D regulation of Src, calcium release and membrane fusion during Xenopus laevis development.
Grant Role of Phosphatidic Acid in the Sperm Acrosome Reaction
Grant Lipid Signaling During Fertilization
Grant A new model for Src tyrosine kinase activation through lipid phosphatidic acid
Academic Article Insemination or phosphatidic acid induces an outwardly spiraling disk of elevated Ca2+ to produce the Ca2+ wave during Xenopus laevis fertilization.

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