JingshiShenJingshi Shen40.00825400000000-105.268079500000003131Shen, Jingshi303/492-6166Professorprns:coAuthorOfcoauthor ofprns:endDateend dateFaculty Rankprns:fullNamefull nameprns:grantAwardedBygrant awarded byprns:hasAuthorListauthor listprns:hasFacultyRankhas faculty rankprns:hasNetworkhas networkprns:hasPublicationVenuepublished inprns:informationResourceReferenceinformation resource referenceprns:isPrimaryPositionis primary positionprns:latitudelatitudeprns:longitudelongitudeprns:maxWeightmaximum weightprns:medlineTAjournal title abbreviationprns:meshDescriptorUIMeSH DescriptorUIprns:meshSemanticGroupNameMeSH semantic group nameprns:minWeightminimum weightprns:numberOfAuthorsnumber of authorsprns:numberOfConnectionsnumber of connectionsprns:numberOfPublicationsnumber of publicationsprns:personIdPerson IDprns:personInPrimaryPositionperson in primary positionprns:physicalNeighborOfphysical neighborprns:positionInDepartmentposition in departmentprns:predicateNodepredicate nodeprns:principalInvestigatorNameprincipal investigator nameprns:publicationDatepublication dateprns:similarTosimilar toprns:sortOrdersort orderprns:startDatestart dateprns:uniquenessWeightuniqueness weightprns:yearyearAcademic ArticleArticleDocumentbibo:pmidPubMed IdentifierAddressvivo:address1address line 1vivo:address2address line 2vivo:address3address line 3vivo:addressCitycityvivo:addressPostalCodepostal codevivo:addressStatestate or provinceAgreementvivo:authorInAuthorshipselected publicationsvivo:authorRankauthor rank in publicationAuthorshipDepartmentGrantvivo:hasResearchArearesearch areasvivo:hasResearcherRoleresearch activitiesvivo:hrJobTitleHR job titleInformation Resourcevivo:linkedAuthorlinked authorvivo:linkedInformationResourcelinked information resourcevivo:mailingAddressmailing addressvivo:personInPositionpositionsvivo:phoneNumberphonePositionvivo:positionInOrganizationposition in organizationvivo:preferredTitlepreferred titleResearcher Rolevivo:researcherRoleOfresearcher role ofRolevivo:roleContributesTocontributes tovivo:sponsorAwardIdsponsor award idrdf:predicatepredicaterdf:typetyperdfs:labellabelConceptAgentfoaf:firstNamefirst namefoaf:lastNamelast nameOrganizationPersonSaraSawyerSara Lea Sawyer40.00825400000000-105.268079500000009570Sawyer, SaraProfessorAuthorship 122193825475060Zhang C, Lee S, Peng Y, Bunker E, Shen C, Giaime E, Shen J, Shen J, Zhou Z, Liu XCell researchZhang C, Lee S, Peng Y, Bunker E, Shen C, Giaime E, Shen J, Shen J, Zhou Z, Liu X. A chemical genetic approach to probe the function of PINK1 in regulating mitochondrial dynamics. Cell Res. 2015 Mar; 25(3):394-7.Cell Res2014-12-05T00:00:002014A chemical genetic approach to probe the function of PINK1 in regulating mitochondrial dynamics.Authorship 126036726074080Davis EM, Kim J, Menasche BL, Sheppard J, Liu X, Tan AC, Shen JCell reportsDavis EM, Kim J, Menasche BL, Sheppard J, Liu X, Tan AC, Shen J. Comparative Haploid Genetic Screens Reveal Divergent Pathways in the Biogenesis and Trafficking of Glycophosphatidylinositol-Anchored Proteins. Cell Rep. 2015 Jun 23; 11(11):1727-36.Cell Rep2015-06-11T00:00:002015Comparative Haploid Genetic Screens Reveal Divergent Pathways in the Biogenesis and Trafficking of Glycophosphatidylinositol-Anchored Proteins.Authorship 132129726431309Yu H, Rathore SS, Shen C, Liu Y, Ouyang Y, Stowell MH, Shen JJournal of the American Chemical SocietyYu H, Rathore SS, Shen C, Liu Y, Ouyang Y, Stowell MH, Shen J. Reconstituting Intracellular Vesicle Fusion Reactions: The Essential Role of Macromolecular Crowding. J Am Chem Soc. 2015 Oct 14; 137(40):12873-83.J Am Chem Soc2015-10-02T00:00:002015Reconstituting Intracellular Vesicle Fusion Reactions: The Essential Role of Macromolecular Crowding.Authorship 134711826572858Shen C, Rathore SS, Yu H, Gulbranson DR, Hua R, Zhang C, Schoppa NE, Shen JNature communicationsShen C, Rathore SS, Yu H, Gulbranson DR, Hua R, Zhang C, Schoppa NE, Shen J. The trans-SNARE-regulating function of Munc18-1 is essential to synaptic exocytosis. Nat Commun. 2015 Nov 17; 6:8852.Nat Commun2015-11-17T00:00:002015The trans-SNARE-regulating function of Munc18-1 is essential to synaptic exocytosis.Authorship 140589627044075Yu H, Liu Y, Gulbranson DR, Paine A, Rathore SS, Shen JProceedings of the National Academy of Sciences of the United States of AmericaYu H, Liu Y, Gulbranson DR, Paine A, Rathore SS, Shen J. Extended synaptotagmins are Ca2+-dependent lipid transfer proteins at membrane contact sites. Proc Natl Acad Sci U S A. 2016 Apr 19; 113(16):4362-7.Proc Natl Acad Sci U S A2016-04-04T00:00:002016Extended synaptotagmins are Ca2+-dependent lipid transfer proteins at membrane contact sites.5Other Faculty1Professor2018-09-19NIHSHEN, JINGSHI2013-04-01Regulatory Mechanisms of GLUT4 ExocytosisR01DK0953672018-01-31NIHSHEN, JINGSHI2013-04-01Protein-Membrane Interactions in Regulated ExocytosisR01GM1022172012-01-31NIHSHEN, JINGSHI2009-02-15Regulation of GLUT4 ExocytosisR00DK0800802020-08-31NIHSHEN, JINGSHI2012-09-21Regulatory Mechanisms of GLUT4 ExocytosisR56DK0953672009-08-31NIHSHEN, JINGSHI2007-09-20Regulation of GLUT4 ExocytosisK99DK080080Principal InvestigatorPrincipal InvestigatorPrincipal InvestigatorPrincipal InvestigatorPrincipal InvestigatorAuthorship 169754728894007Gulbranson DR, Davis EM, Demmitt BA, Ouyang Y, Ye Y, Yu H, Shen JProceedings of the National Academy of Sciences of the United States of AmericaGulbranson DR, Davis EM, Demmitt BA, Ouyang Y, Ye Y, Yu H, Shen J. RABIF/MSS4 is a Rab-stabilizing holdase chaperone required for GLUT4 exocytosis. Proc Natl Acad Sci U S A. 2017 09 26; 114(39):E8224-E8233.Proc Natl Acad Sci U S A2017-09-11T00:00:002017RABIF/MSS4 is a Rab-stabilizing holdase chaperone required for GLUT4 exocytosis.2020-07-31NIHLI, SUZHAO2017-08-14Molecular Mechanisms of Cytokine Induced Insulin ResistanceR21AI128443Co-Principal Investigator21139055Rathore SS, Bend EG, Yu H, Hammarlund M, Jorgensen EM, Shen JProceedings of the National Academy of Sciences of the United States of AmericaRathore SS, Bend EG, Yu H, Hammarlund M, Jorgensen EM, Shen J. Syntaxin N-terminal peptide motif is an initiation factor for the assembly of the SNARE-Sec1/Munc18 membrane fusion complex. Proc Natl Acad Sci U S A. 2010 Dec 28; 107(52):22399-406.Proc Natl Acad Sci U S A2010-12-07T00:00:002010Syntaxin N-terminal peptide motif is an initiation factor for the assembly of the SNARE-Sec1/Munc18 membrane fusion complex.29090903Guan X, Chaffey PK, Wei X, Gulbranson DR, Ruan Y, Wang X, Li Y, Ouyang Y, Chen L, Zeng C, Koelsch TN, Tran AH, Liang W, Shen J, Tan ZACS chemical biologyGuan X, Chaffey PK, Wei X, Gulbranson DR, Ruan Y, Wang X, Li Y, Ouyang Y, Chen L, Zeng C, Koelsch TN, Tran AH, Liang W, Shen J, Tan Z. Chemically Precise Glycoengineering Improves Human Insulin. ACS Chem Biol. 2018 01 19; 13(1):73-81.ACS Chem Biol2017-12-01T00:00:002017Chemically Precise Glycoengineering Improves Human Insulin.23427263Yu H, Rathore SS, Davis EM, Ouyang Y, Shen JMolecular biology of the cellYu H, Rathore SS, Davis EM, Ouyang Y, Shen J. Doc2b promotes GLUT4 exocytosis by activating the SNARE-mediated fusion reaction in a calcium- and membrane bending-dependent manner. Mol Biol Cell. 2013 Apr; 24(8):1176-84.Mol Biol Cell2013-02-20T00:00:002013Doc2b promotes GLUT4 exocytosis by activating the SNARE-mediated fusion reaction in a calcium- and membrane bending-dependent manner.1945 Colorado Ave.1MCDBGold Biosciences Building1945 Colorado Ave.Boulder80309-0001CO20603329Shen J, Rathore SS, Khandan L, Rothman JEThe Journal of cell biologyShen J, Rathore SS, Khandan L, Rothman JE. SNARE bundle and syntaxin N-peptide constitute a minimal complement for Munc18-1 activation of membrane fusion. J Cell Biol. 2010 Jul 12; 190(1):55-63.J Cell Biol2010-07-05T00:00:002010SNARE bundle and syntaxin N-peptide constitute a minimal complement for Munc18-1 activation of membrane fusion.21633111Rathore SS, Ghosh N, Ouyang Y, Shen JMolecular biology of the cellRathore SS, Ghosh N, Ouyang Y, Shen J. Topological arrangement of the intracellular membrane fusion machinery. Mol Biol Cell. 2011 Jul 15; 22(14):2612-9.Mol Biol Cell2011-06-01T00:00:002011Topological arrangement of the intracellular membrane fusion machinery.Authorship 172875142024-03-31NIHSHEN, JINGSHI2018-04-01Protein-membrane interactions in regulated exocytosisR35GM126960Principal Investigator2020-04-30NIH/NIAIDYU, HAIJIA2018-05-03Dissect Cytolytic Granule Secretion in Cytotoxic T Lymphocytes Using CRISPR Genetic ScreensR03AI135473Co-Principal InvestigatorAuthorship 188977630265447Menasche BL, Crisman L, Gulbranson DR, Davis EM, Yu H, Shen JCurrent protocols in cell biologyMenasche BL, Crisman L, Gulbranson DR, Davis EM, Yu H, Shen J. Fluorescence Activated Cell Sorting (FACS) in Genome-Wide Genetic Screening of Membrane Trafficking. Curr Protoc Cell Biol. 2019 03; 82(1):e68.Curr Protoc Cell Biol2018-09-28T00:00:002018Fluorescence Activated Cell Sorting (FACS) in Genome-Wide Genetic Screening of Membrane Trafficking.Authorship 189100930275014Shen C, Liu Y, Yu H, Gulbranson DR, Kogut I, Bilousova G, Zhang C, Stowell MHB, Shen JThe Journal of biological chemistryShen C, Liu Y, Yu H, Gulbranson DR, Kogut I, Bilousova G, Zhang C, Stowell MHB, Shen J. The N-peptide-binding mode is critical to Munc18-1 function in synaptic exocytosis. J Biol Chem. 2018 11 23; 293(47):18309-18317.J Biol Chem2018-10-01T00:00:002018The N-peptide-binding mode is critical to Munc18-1 function in synaptic exocytosis.D002462Anatomy2917410.737237Cell MembraneD008561Physiology21400.964905Membrane FusionD050600Chemicals & Drugs20370.96783SNARE ProteinsAuthorship 97362323665562Yu H, Rathore SS, Shen JThe Journal of biological chemistryYu H, Rathore SS, Shen J. Synip arrests soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE)-dependent membrane fusion as a selective target membrane SNARE-binding inhibitor. J Biol Chem. 2013 Jun 28; 288(26):18885-93.J Biol Chem2013-05-12T00:00:002013Synip arrests soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE)-dependent membrane fusion as a selective target membrane SNARE-binding inhibitor.ChunWanChun Wan40.00825400000000-105.2680795000000015167Wan, ChunResearch Associate2023-03-31NIHSHEN, JINGSHI2018-09-30Identifying exosome-mediated cargo transport machinery in Alzheimer's diseaseRF1AG061829Principal InvestigatorSOM-BDC AdultD005089Physiology53900.941952ExocytosisD051938Chemicals & Drugs10230.973464Munc18 ProteinsSOM-PHARM General OperationsBiology-MCD InstructionBiochemistrySOM-Cell and Devopmental BioUniversity of Colorado BoulderUniversity of Colorado Denver - Anschutz Medical CampusTatianaKutateladzeTatiana Grigori Kutateladze39.74545800000000-104.839369100000001029Kutateladze, TatianaProfessorXuedongLiuXuedong Liu40.00830860000000-105.249067200000001467Liu, XuedongProfessorMichaelStowellMichael Stowell40.00825400000000-105.268079500000001678Stowell, MichaelProfessorRytisPrekerisRytis Prekeris39.74389450000000-104.839435200000001613Prekeris, RytisProfessorBarbaraFrederickBarbara Anne Frederick40.00825400000000-105.268079500000001515Frederick, BarbaraSr Research AssociateThomasPerkinsThomas T. Perkins40.00825400000000-105.268079500000001696Perkins, ThomasProfessor AdjointCorrellaDetweilerCorrella Scott Detweiler40.00825400000000-105.268079500000001913Detweiler, CorrellaProfessorMatthewKennedyMatthew James Kennedy39.74545800000000-104.839369100000004146Kennedy, MatthewProfessorSatishGargSatish K Garg39.74404140000000-104.83839450000000961Garg, SatishProfessorGiaVoeltzGia Kaarina Voeltz40.00825400000000-105.268079500000002557Voeltz, GiaProfessor4.565720.0156056155research areas2.845030.032777713coauthor of26.79865.9224360similar to1127selected publicationsJosephFalkeJoseph Falke40.00830860000000-105.24906720000000476Falke, JosephProfessorMingxueCuiMingxue Cui40.00825400000000-105.26807950000000640Cui, MingxueResearch AssociateAuthorship 2050841131353312Gulbranson DR, Crisman L, Lee M, Ouyang Y, Menasche BL, Demmitt BA, Wan C, Nomura T, Ye Y, Yu H, Shen JDevelopmental cellGulbranson DR, Crisman L, Lee M, Ouyang Y, Menasche BL, Demmitt BA, Wan C, Nomura T, Ye Y, Yu H, Shen J. AAGAB Controls AP2 Adaptor Assembly in Clathrin-Mediated Endocytosis. Dev Cell. 2019 08 19; 50(4):436-446.e5.Dev Cell2019-07-25T00:00:002019AAGAB Controls AP2 Adaptor Assembly in Clathrin-Mediated Endocytosis.ChristinaWinbornChristina Sonshine Winborn16150Winborn, ChristinaResearch AssociateAuthorship 212178931740584Wang S, Crisman L, Miller J, Datta I, Gulbranson DR, Tian Y, Yin Q, Yu H, Shen JThe Journal of biological chemistryWang S, Crisman L, Miller J, Datta I, Gulbranson DR, Tian Y, Yin Q, Yu H, Shen J. Inducible Exoc7/Exo70 knockout reveals a critical role of the exocyst in insulin-regulated GLUT4 exocytosis. J Biol Chem. 2019 Dec 27; 294(52):19988-19996.J Biol Chem2019-11-18T00:00:002019Inducible Exoc7/Exo70 knockout reveals a critical role of the exocyst in insulin-regulated GLUT4 exocytosis.Authorship 213430831875562Rathore SS, Liu Y, Yu H, Wan C, Lee M, Yin Q, Stowell MHB, Shen JCell reportsRathore SS, Liu Y, Yu H, Wan C, Lee M, Yin Q, Stowell MHB, Shen J. Intracellular Vesicle Fusion Requires a Membrane-Destabilizing Peptide Located at the Juxtamembrane Region of the v-SNARE. Cell Rep. 2019 12 24; 29(13):4583-4592.e3.Cell Rep2019-12-24T00:00:002019Intracellular Vesicle Fusion Requires a Membrane-Destabilizing Peptide Located at the Juxtamembrane Region of the v-SNARE.Authorship 99917723918365Yu H, Rathore SS, Lopez JA, Davis EM, James DE, Martin JL, Shen JProceedings of the National Academy of Sciences of the United States of AmericaYu H, Rathore SS, Lopez JA, Davis EM, James DE, Martin JL, Shen J. Comparative studies of Munc18c and Munc18-1 reveal conserved and divergent mechanisms of Sec1/Munc18 proteins. Proc Natl Acad Sci U S A. 2013 Aug 27; 110(35):E3271-80.Proc Natl Acad Sci U S A2013-08-05T00:00:002013Comparative studies of Munc18c and Munc18-1 reveal conserved and divergent mechanisms of Sec1/Munc18 proteins.2024-03-31NIHSHEN, JINGSHI2020-04-01Mechanisms of GLUT4 ExocytosisR01DK124431Principal InvestigatorAuthorship 228167732851733Wang S, Liu Y, Crisman L, Wan C, Miller J, Yu H, Shen JTraffic (Copenhagen, Denmark)Wang S, Liu Y, Crisman L, Wan C, Miller J, Yu H, Shen J. Genetic evidence for an inhibitory role of tomosyn in insulin-stimulated GLUT4 exocytosis. Traffic. 2020 10; 21(10):636-646.Traffic2020-10-01T00:00:002020Genetic evidence for an inhibitory role of tomosyn in insulin-stimulated GLUT4 exocytosis.Authorship 2305267Authorship 2317057Authorship 231700933246952Menasche BL, Davis EM, Wang S, Ouyang Y, Li S, Yu H, Shen JScience advancesMenasche BL, Davis EM, Wang S, Ouyang Y, Li S, Yu H, Shen J. PBRM1 and the glycosylphosphatidylinositol biosynthetic pathway promote tumor killing mediated by MHC-unrestricted cytotoxic lymphocytes. Sci Adv. 2020 11; 6(48).Sci Adv2020-11-27T00:00:002020PBRM1 and the glycosylphosphatidylinositol biosynthetic pathway promote tumor killing mediated by MHC-unrestricted cytotoxic lymphocytes.33290418Dombach JL, Quintana JLJ, Nagy TA, Wan C, Crooks AL, Yu H, Su CC, Yu EW, Shen J, Detweiler CSPLoS pathogensDombach JL, Quintana JLJ, Nagy TA, Wan C, Crooks AL, Yu H, Su CC, Yu EW, Shen J, Detweiler CS. A small molecule that mitigates bacterial infection disrupts Gram-negative cell membranes and is inhibited by cholesterol and neutral lipids. PLoS Pathog. 2020 12; 16(12):e1009119.PLoS Pathog2020-12-08T00:00:002020A small molecule that mitigates bacterial infection disrupts Gram-negative cell membranes and is inhibited by cholesterol and neutral lipids.33296682Zhang X, Xu Q, Zi Z, Liu Z, Wan C, Crisman L, Shen J, Liu XDevelopmental cellZhang X, Xu Q, Zi Z, Liu Z, Wan C, Crisman L, Shen J, Liu X. Programmable Extracellular Vesicles for Macromolecule Delivery and Genome Modifications. Dev Cell. 2020 12 21; 55(6):784-801.e9.Dev Cell2020-12-08T00:00:002020Programmable Extracellular Vesicles for Macromolecule Delivery and Genome Modifications.Authorship 234437633440145Liu Y, Wan C, Rathore SS, Stowell MHB, Yu H, Shen JCell reportsLiu Y, Wan C, Rathore SS, Stowell MHB, Yu H, Shen J. SNARE Zippering Is Suppressed by a Conformational Constraint that Is Removed by v-SNARE Splitting. Cell Rep. 2021 01 12; 34(2):108611.Cell Rep2021-01-12T00:00:002021SNARE Zippering Is Suppressed by a Conformational Constraint that Is Removed by v-SNARE Splitting.Authorship 2491221234494650Wan C, Crisman L, Wang B, Tian Y, Wang S, Yang R, Datta I, Nomura T, Li S, Yu H, Yin Q, Shen JJournal of cell scienceWan C, Crisman L, Wang B, Tian Y, Wang S, Yang R, Datta I, Nomura T, Li S, Yu H, Yin Q, Shen J. AAGAB is an assembly chaperone regulating AP1 and AP2 clathrin adaptors. J Cell Sci. 2021 10 01; 134(19).J Cell Sci2021-10-01T00:00:002021AAGAB is an assembly chaperone regulating AP1 and AP2 clathrin adaptors.Authorship 274076336369335Wan C, Stowell MHB, Shen JCommunications biologyWan C, Stowell MHB, Shen J. Progress and gaps of extracellular vesicle-mediated intercellular cargo transfer in the central nervous system. Commun Biol. 2022 Nov 11; 5(1):1223.Commun Biol2022-11-11T00:00:002022Progress and gaps of extracellular vesicle-mediated intercellular cargo transfer in the central nervous system.Authorship 2774751036812304Wang S, Chen X, Crisman L, Dou X, Winborn CS, Wan C, Puscher H, Yin Q, Kennedy MJ, Shen JScience advancesWang S, Chen X, Crisman L, Dou X, Winborn CS, Wan C, Puscher H, Yin Q, Kennedy MJ, Shen J. Regulation of cargo exocytosis by a Reps1-Ralbp1-RalA module. Sci Adv. 2023 02 22; 9(8):eade2540.Sci Adv2023-02-22T00:00:002023Regulation of cargo exocytosis by a Reps1-Ralbp1-RalA module.true1ProfessorProfessortrue1ProfessorProfessortrue1Professor AdjointProfessor Adjointtrue1ProfessorProfessortrue1ProfessorProfessortrue1Research AssociateResearch Associatetrue1ProfessorProfessortrue1Research AssociateResearch Associatetrue1ProfessorProfessortrue1ProfessorProfessortrue1Research AssociateResearch Associatetrue1ProfessorProfessortrue1ProfessorProfessortrue1Sr Research AssociateSr Research Associatetrue1ProfessorProfessortrue1ProfessorProfessorAuthorship 477996Authorship 505451Authorship 630055Authorship 115953425063806Yu H, Rathore SS, Gulbranson DR, Shen JThe Journal of biological chemistryYu H, Rathore SS, Gulbranson DR, Shen J. The N- and C-terminal domains of tomosyn play distinct roles in soluble N-ethylmaleimide-sensitive factor attachment protein receptor binding and fusion regulation. J Biol Chem. 2014 Sep 12; 289(37):25571-80.J Biol Chem2014-07-25T00:00:002014The N- and C-terminal domains of tomosyn play distinct roles in soluble N-ethylmaleimide-sensitive factor attachment protein receptor binding and fusion regulation.Authorship 742014