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Joel M Kralj

TitleAsst Professor
InstitutionUniversity of Colorado Boulder
DepartmentBiology-MCD Instruction

    Collapse Research 
    Collapse research activities and funding
    R43NS087714     (MARGULIES, DAVID)Apr 1, 2014 - Mar 31, 2016
    Optopatch: high-throughput all-optical electrophysiology for neural recording
    Role: Co-Principal Investigator

    DP2GM123458     (KRALJ, JOEL MARK)Sep 30, 2016 - May 31, 2021
    Neuronal electromics in health and disease
    Role: Principal Investigator

    Collapse Bibliographic 
    Collapse selected publications
    Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Faculty can login to make corrections and additions.
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    1. Kiskinis E, Kralj JM, Zou P, Weinstein EN, Zhang H, Tsioras K, Wiskow O, Ortega JA, Eggan K, Cohen AE. All-Optical Electrophysiology for High-Throughput Functional Characterization of a Human iPSC-Derived Motor Neuron Model of ALS. Stem Cell Reports. 2018 Jun 05; 10(6):1991-2004. PMID: 29779896.
      View in: PubMed
    2. Dodd BJT, Kralj JM. Live Cell Imaging Reveals pH Oscillations in Saccharomyces cerevisiae During Metabolic Transitions. Sci Rep. 2017 Oct 24; 7(1):13922. PMID: 29066766.
      View in: PubMed
    3. Bruni GN, Weekley RA, Dodd BJT, Kralj JM. Voltage-gated calcium flux mediates Escherichia coli mechanosensation. Proc Natl Acad Sci U S A. 2017 08 29; 114(35):9445-9450. PMID: 28808010.
      View in: PubMed
    4. Werley CA, Chien MP, Gaublomme J, Shekhar K, Butty V, Yi BA, Kralj JM, Bloxham W, Boyer LA, Regev A, Cohen AE. Geometry-dependent functional changes in iPSC-derived cardiomyocytes probed by functional imaging and RNA sequencing. PLoS One. 2017; 12(3):e0172671. PMID: 28333933.
      View in: PubMed
    5. Dempsey GT, Chaudhary KW, Atwater N, Nguyen C, Brown BS, McNeish JD, Cohen AE, Kralj JM. Cardiotoxicity screening with simultaneous optogenetic pacing, voltage imaging and calcium imaging. J Pharmacol Toxicol Methods. 2016 Sep-Oct; 81:240-50. PMID: 27184445.
      View in: PubMed
    6. Hou JH, Kralj JM, Douglass AD, Engert F, Cohen AE. Simultaneous mapping of membrane voltage and calcium in zebrafish heart in vivo reveals chamber-specific developmental transitions in ionic currents. Front Physiol. 2014; 5:344. PMID: 25309445; PMCID: PMC4161048.
    7. Hochbaum DR, Zhao Y, Farhi SL, Klapoetke N, Werley CA, Kapoor V, Zou P, Kralj JM, Maclaurin D, Smedemark-Margulies N, Saulnier JL, Boulting GL, Straub C, Cho YK, Melkonian M, Wong GK, Harrison DJ, Murthy VN, Sabatini BL, Boyden ES, Campbell RE, Cohen AE. All-optical electrophysiology in mammalian neurons using engineered microbial rhodopsins. Nat Methods. 2014 Aug; 11(8):825-33. PMID: 24952910; PMCID: PMC4117813.
    8. Venkatachalam V, Brinks D, Maclaurin D, Hochbaum D, Kralj J, Cohen AE. Flash memory: photochemical imprinting of neuronal action potentials onto a microbial rhodopsin. J Am Chem Soc. 2014 Feb 12; 136(6):2529-37. PMID: 24428326; PMCID: PMC3985752.
    9. Park J, Werley CA, Venkatachalam V, Kralj JM, Dib-Hajj SD, Waxman SG, Cohen AE. Screening fluorescent voltage indicators with spontaneously spiking HEK cells. PLoS One. 2013; 8(12):e85221. PMID: 24391999; PMCID: PMC3877367.
    10. Saint Clair EC, Ogren JI, Mamaev S, Russano D, Kralj JM, Rothschild KJ. Near-IR resonance Raman spectroscopy of archaerhodopsin 3: effects of transmembrane potential. J Phys Chem B. 2012 Dec 20; 116(50):14592-601. PMID: 23189985; PMCID: PMC3568500.
    11. Clair EC, Ogren JI, Mamaev S, Kralj JM, Rothschild KJ. Conformational changes in the archaerhodopsin-3 proton pump: detection of conserved strongly hydrogen bonded water networks. J Biol Phys. 2012 Jan; 38(1):153-68. PMID: 23277676; PMCID: PMC3285725.
    12. Kralj JM, Douglass AD, Hochbaum DR, Maclaurin D, Cohen AE. Optical recording of action potentials in mammalian neurons using a microbial rhodopsin. Nat Methods. 2011 Nov 27; 9(1):90-5. PMID: 22120467; PMCID: PMC3248630.
    13. Bayraktar H, Fields AP, Kralj JM, Spudich JL, Rothschild KJ, Cohen AE. Ultrasensitive measurements of microbial rhodopsin photocycles using photochromic FRET. Photochem Photobiol. 2012 Jan-Feb; 88(1):90-7. PMID: 22010969; PMCID: PMC3253248.
    14. Kralj JM, Hochbaum DR, Douglass AD, Cohen AE. Electrical spiking in Escherichia coli probed with a fluorescent voltage-indicating protein. Science. 2011 Jul 15; 333(6040):345-8. PMID: 21764748.
      View in: PubMed
    15. Bergo VB, Sineshchekov OA, Kralj JM, Partha R, Spudich EN, Rothschild KJ, Spudich JL. His-75 in proteorhodopsin, a novel component in light-driven proton translocation by primary pumps. J Biol Chem. 2009 Jan 30; 284(5):2836-43. PMID: 19015272; PMCID: PMC2631968.
    16. Amsden JJ, Kralj JM, Bergo VB, Spudich EN, Spudich JL, Rothschild KJ. Different structural changes occur in blue- and green-proteorhodopsins during the primary photoreaction. Biochemistry. 2008 Nov 04; 47(44):11490-8. PMID: 18842006; PMCID: PMC3631532.
    17. Kralj JM, Spudich EN, Spudich JL, Rothschild KJ. Raman spectroscopy reveals direct chromophore interactions in the Leu/Gln105 spectral tuning switch of proteorhodopsins. J Phys Chem B. 2008 Sep 18; 112(37):11770-6. PMID: 18717545; PMCID: PMC3608850.
    18. Cappuccio JA, Blanchette CD, Sulchek TA, Arroyo ES, Kralj JM, Hinz AK, Kuhn EA, Chromy BA, Segelke BW, Rothschild KJ, Fletcher JE, Katzen F, Peterson TC, Kudlicki WA, Bench G, Hoeprich PD, Coleman MA. Cell-free co-expression of functional membrane proteins and apolipoprotein, forming soluble nanolipoprotein particles. Mol Cell Proteomics. 2008 Nov; 7(11):2246-53. PMID: 18603642; PMCID: PMC2577204.
    19. Kralj JM, Bergo VB, Amsden JJ, Spudich EN, Spudich JL, Rothschild KJ. Protonation state of Glu142 differs in the green- and blue-absorbing variants of proteorhodopsin. Biochemistry. 2008 Mar 18; 47(11):3447-53. PMID: 18284210.
      View in: PubMed
    20. Amsden JJ, Kralj JM, Chieffo LR, Wang X, Erramilli S, Spudich EN, Spudich JL, Ziegler LD, Rothschild KJ. Subpicosecond protein backbone changes detected during the green-absorbing proteorhodopsin primary photoreaction. J Phys Chem B. 2007 Oct 11; 111(40):11824-31. PMID: 17880126.
      View in: PubMed
    21. Bergo VB, Ntefidou M, Trivedi VD, Amsden JJ, Kralj JM, Rothschild KJ, Spudich JL. Conformational changes in the photocycle of Anabaena sensory rhodopsin: absence of the Schiff base counterion protonation signal. J Biol Chem. 2006 Jun 02; 281(22):15208-14. PMID: 16537532.
      View in: PubMed
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