Bradley J Stith
Title | Professor |
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Institution | University of Colorado Denver - Denver Campus |
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Department | CLAS-Biology |
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Phone | 303/315-7669 |
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Biography 2009 | Excellence in Research Award, College of Liberal Arts and Sciences, University of Colorado Denver | 2008 | Faculty Award for Outstanding Student Mentoring; Research and Creative Activities Symposium, University of Colorado Denver | 2007 | Excellence in Service Award, College of Liberal Arts and Sciences, University of Colorado Denver |
Overview We have suggested a new step in fertilization involving Src tyrosine kinase activation by a lipid called phosphatidic acid (PA). Large-scale HPLC/ELSD quantification of lipids at fertilization (Petcoff et al. J Lipid Res 2365-78, 2008) suggests that production of PA through the action of phospholipase D has a role in fertilization. In an action that requires intact membrane rafts, PA activates Src tyrosine kinase, which then activates phospholipase C (PLC?) to produce IP3 and subsequent intracellular calcium release to induce fertilization in Xenopus laevis (Stith, Developmental Biology 401(2):188–205; Bates et al., Developmental Biology 386:165-180, 2014). This work suggests a new path to cancer as PA is elevated and Src is hyperactivated in many forms of cancer. Based on our lipid-Src binding work, studies by Stuart McLaughlin (SUNY Stony Brook) and Miguel Pons (Univ of Barcelona), anionic lipids may bind to the SH4 domain, the ULBR and SH3 domains of Src to “loosen” the tight configuration of Src to lead to its activation. Support: NIH (HD065661) and the University of Colorado Denver Undergraduate Research Opportunity Program
Research F32HD006636 (STITH, BRADLEY J)Jul 15, 1985 NIH/NICHD PROTEIN PHOSPHATE/TYR-KINASE REGULATION BY INSULIN Role: Principal Investigator |
| R15HD052546 (STITH, BRADLEY J)May 1, 2006 - May 12, 2008 NIH/NICHD Role of Phosphatidic Acid in the Sperm Acrosome Reaction Role: Principal Investigator |
| R15HD065661 (STITH, BRADLEY J)May 1, 2010 - Sep 30, 2013 NIH/NICHD Lipid Signaling During Fertilization Role: Principal Investigator |
| R15GM122036 (STITH, BRADLEY J)Sep 23, 2016 - Aug 31, 2019 NIH/NIGMS A new model for Src tyrosine kinase activation through lipid phosphatidic acid Role: Principal Investigator |
Bibliographic
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Fees CP, Stith BJ. Insemination or phosphatidic acid induces an outwardly spiraling disk of elevated Ca2+ to produce the Ca2+ wave during Xenopus laevis fertilization. Dev Biol. 2019 04 01; 448(1):59-68. PMID: 30641042.
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Bates RC, Stith BJ, Stevens KE. Increasing pro-survival factors within whole brain tissue of Sprague Dawley rats via intracerebral administration of modified valproic acid. J Pharmacol Sci. 2015 Aug; 128(4):193-201. PMID: 26275469.
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Stith BJ. Phospholipase C and D regulation of Src, calcium release and membrane fusion during Xenopus laevis development. Dev Biol. 2015 May 15; 401(2):188-205. PMID: 25748412.
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Bradley J. Stith. Phospholipase C and D regulation of Src, calcium release and membrane fusion during Xenopus laevis development. Developmental Biology. 2015; 401:188-205.
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Bates RC, Stith BJ, Stevens KE, Adams CE. Reduced CHRNA7 expression in C3H mice is associated with increases in hippocampal parvalbumin and glutamate decarboxylase-67 (GAD67) as well as altered levels of GABA(A) receptor subunits. Neuroscience. 2014 Jul 25; 273:52-64. PMID: 24836856.
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Bates RC, Fees CP, Holland WL, Winger CC, Batbayar K, Ancar R, Bergren T, Petcoff D, Stith BJ. Activation of Src and release of intracellular calcium by phosphatidic acid during Xenopus laevis fertilization. Dev Biol. 2014 Feb 01; 386(1):165-80. PMID: 24269904.
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Bates RC, Stith BJ, Stevens KE. Chronic central administration of valproic acid: Increased pro-survival phospho-proteins and growth cone associated proteins with no behavioral pathology. Pharmacol Biochem Behav. 2012 Dec; 103(2):237-44. PMID: 22960225.
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Petcoff DW, Holland WL, Stith BJ. Lipid levels in sperm, eggs, and during fertilization in Xenopus laevis. J Lipid Res. 2008 Nov; 49(11):2365-78. PMID: 18577769.
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Sato K, Fukami Y, Stith BJ. Signal transduction pathways leading to Ca2+ release in a vertebrate model system: lessons from Xenopus eggs. Semin Cell Dev Biol. 2006 Apr; 17(2):285-92. PMID: 16584903.
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Holland W, Morrison T, Chang Y, Wiernsperger N, Stith BJ. Metformin (Glucophage) inhibits tyrosine phosphatase activity to stimulate the insulin receptor tyrosine kinase. Biochem Pharmacol. 2004 Jun 01; 67(11):2081-91. PMID: 15135305.
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Stith BJ. Use of animation in teaching cell biology. Cell Biol Educ. 2004; 3(3):181-8. PMID: 15526065.
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Holland WL, Stauter EC, Stith BJ. Quantification of phosphatidic acid and lysophosphatidic acid by HPLC with evaporative light-scattering detection. J Lipid Res. 2003 Apr; 44(4):854-8. PMID: 12562857.
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Luria A, Vegelyte-Avery V, Stith B, Tsvetkova NM, Wolkers WF, Crowe JH, Tablin F, Nuccitelli R. Detergent-free domain isolated from Xenopus egg plasma membrane with properties similar to those of detergent-resistant membranes. Biochemistry. 2002 Nov 05; 41(44):13189-97. PMID: 12403620.
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Stith BJ, Hall J, Ayres P, Waggoner L, Moore JD, Shaw WA. Quantification of major classes of Xenopus phospholipids by high performance liquid chromatography with evaporative light scattering detection. J Lipid Res. 2000 Sep; 41(9):1448-54. PMID: 10974052.
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Morrison T, Waggoner L, Whitworth-Langley L, Stith BJ. Nongenomic action of progesterone: activation of Xenopus oocyte phospholipase C through a plasma membrane-associated tyrosine kinase. Endocrinology. 2000 Jun; 141(6):2145-52. PMID: 10830302.
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Stith BJ, Woronoff K, Wiernsperger N. Stimulation of the intracellular portion of the human insulin receptor by the antidiabetic drug metformin. Biochem Pharmacol. 1998 Feb 15; 55(4):533-6. PMID: 9514089.
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Stith BJ, Woronoff K, Espinoza R, Smart T. sn-1,2-diacylglycerol and choline increase after fertilization in Xenopus laevis. Mol Biol Cell. 1997 Apr; 8(4):755-65. PMID: 9247652.
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Stith BJ, Goalstone ML, Espinoza R, Mossel C, Roberts D, Wiernsperger N. The antidiabetic drug metformin elevates receptor tyrosine kinase activity and inositol 1,4,5-trisphosphate mass in Xenopus oocytes. Endocrinology. 1996 Jul; 137(7):2990-9. PMID: 8770923.
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Stith BJ, Espinoza R, Roberts D, Smart T. Sperm increase inositol 1,4,5-trisphosphate mass in Xenopus laevis eggs preinjected with calcium buffers or heparin. Dev Biol. 1994 Sep; 165(1):206-15. PMID: 8088439.
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Stith BJ, Goalstone M, Silva S, Jaynes C. Inositol 1,4,5-trisphosphate mass changes from fertilization through first cleavage in Xenopus laevis. Mol Biol Cell. 1993 Apr; 4(4):435-43. PMID: 8507898.
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Stith BJ, Goalstone ML, Kirkwood AJ. Protein kinase C initially inhibits the induction of meiotic cell division in Xenopus oocytes. Cell Signal. 1992 Jul; 4(4):393-403. PMID: 1419482.
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Stith BJ, Jaynes C, Goalstone M, Silva S. Insulin and progesterone increase 32PO4-labeling of phospholipids and inositol 1,4,5-trisphosphate mass in Xenopus oocytes. Cell Calcium. 1992 May; 13(5):341-52. PMID: 1320459.
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Stith BJ, Kirkwood AJ, Wohnlich E. Insulin-like growth factor 1, insulin, and progesterone induce early and late increases in Xenopus oocyte sn-1,2-diacylglycerol levels before meiotic cell division. J Cell Physiol. 1991 Nov; 149(2):252-9. PMID: 1748718.
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Stith BJ, Proctor WR. Microinjection of inositol 1,2-(cyclic)-4,5-trisphosphate, inositol 1,3,4,5-tetrakisphosphate, and inositol 1,4,5-trisphosphate into intact Xenopus oocytes can induce membrane currents independent of extracellular calcium. J Cell Biochem. 1989 Jul; 40(3):321-30. PMID: 2550488.
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Stith BJ, Maller JL. Induction of meiotic maturation in Xenopus oocytes by 12-O-tetradecanoylphorbol 13-acetate. Exp Cell Res. 1987 Apr; 169(2):514-23. PMID: 3549336.
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Maller JL, Pike LJ, Freidenberg GR, Cordera R, Stith BJ, Olefsky JM, Krebs EG. Increased phosphorylation of ribosomal protein S6 following microinjection of insulin receptor-kinase into Xenopus oocytes. Nature. 1986 Apr 3-9; 320(6061):459-61. PMID: 3083266.
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Stith BJ, Maller JL. Increased intracellular pH is not necessary for ribosomal protein s6 phosphorylation, increased protein synthesis, or germinal vesicle breakdown in Xenopus oocytes. Dev Biol. 1985 Feb; 107(2):460-9. PMID: 3882483.
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Stith BJ, Maller JL. The effect of insulin on intracellular ph and ribosomal protein S6 phosphorylation in oocytes of Xenopus laevis. Dev Biol. 1984 Mar; 102(1):79-89. PMID: 6365656.
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Year | Publications |
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1991 | 1 | 1992 | 2 | 1993 | 1 | 1994 | 1 | 1996 | 1 | 1997 | 1 | 1998 | 1 | 2000 | 2 | 2002 | 1 | 2003 | 1 | 2004 | 2 | 2006 | 1 | 2008 | 1 | 2012 | 1 | 2013 | 1 | 2014 | 1 | 2015 | 3 | 2019 | 1 |
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