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Richard E Davis

TitleProfessor
InstitutionUniversity of Colorado Denver - Anschutz Medical Campus
DepartmentSOM-BioChem&Molecular Genetics
Phone303/724-3226

    Collapse Overview 
    Collapse overview
    The Davis Lab studies the molecular biology of parasitic worms (primarily schistosomes and parasitic nematodes) with a focus on atypical molecular processes including spliced leader RNA trans-splicing and programmed DNA elimination. Our previous studies on trans-splicing used cell-free in vitro systems, biolistic RNA transfection, enzymatic, and structural biology approaches to examine the cellular and biochemical adaptations of mRNA translation and decay to trans-splicing. Current studies are focused on programmed DNA elimination in parasitic nematodes, a phenomenon where chromosomes break and regions of chromosomes are reproducibly lost during early development. We use a variety of approaches to study DNA elimination including genomics and transcriptomics, biochemistry, analysis of small RNAs and Argonautes, chromatin and chromosome analyses, and analysis of chromosome segregation.


    Collapse Research 
    Collapse research activities and funding
    F32AM007086     (DAVIS, RICHARD E.)Feb 1, 1985
    NIH/NIADDK
    STUDY OF THE OPRTASE-ODCASE COMPLEX AND GENE(S)
    Role: Principal Investigator

    R15AI032709     (DAVIS, RICHARD E.)Jun 1, 1992 - May 31, 1996
    NIH/NIAID
    TRANS-SPLICING IN PARASITIC FLATWORMS
    Role: Principal Investigator

    R15AI039714     (DAVIS, RICHARD E.)Jul 1, 1995 - Jun 30, 1998
    NIH/NIAID
    TRANS SPLICING AND PRIMARY TRANSCRIPTS IN ACARIS
    Role: Principal Investigator

    R15AI043300     (DAVIS, RICHARD E.)Jul 1, 1998 - Mar 31, 2000
    NIH/NIAID
    ASCARIS MRNA PROCESSING--EXON/INTRON DEFINITION AND CAP
    Role: Principal Investigator

    R01AI049558     (DAVIS, RICHARD E.)Aug 1, 2000 - May 31, 2016
    NIH/NIAID
    Cap-interacting proteins in metazoan trans-splicing
    Role: Principal Investigator

    R21AI078087     (DAVIS, RICHARD E.)Jun 15, 2008 - May 31, 2011
    NIH/NIAID
    Small RNA Discovery and Analysis in Ascaris
    Role: Principal Investigator

    R03AI080805     (DAVIS, RICHARD E.)Feb 15, 2009 - Jan 31, 2012
    NIH/NIAID
    Structural Analysis of Helminth mRNA Cap-Binding Proteins
    Role: Principal Investigator

    R21AI098421     (DAVIS, RICHARD E.)Feb 15, 2012 - Jan 31, 2014
    NIH/NIAID
    Chromatin Diminution in Ascaris
    Role: Principal Investigator

    R01AI114054     (DAVIS, RICHARD E.)Aug 20, 2015 - Jan 31, 2020
    NIH/NIAID
    Chromatin diminution in nematodes
    Role: Principal Investigator

    Collapse Bibliographic 
    Collapse selected publications
    Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Faculty can login to make corrections and additions.
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    1. Kang Y, Wang J, Neff A, Kratzer S, Kimura H, Davis RE. Differential Chromosomal Localization of Centromeric Histone CENP-A Contributes to Nematode Programmed DNA Elimination. Cell Rep. 2016 Aug 30; 16(9):2308-16. PMID: 27545882; PMCID: PMC5007152.
    2. Streit A, Wang J, Kang Y, Davis RE. Gene silencing and sex determination by programmed DNA elimination in parasitic nematodes. Curr Opin Microbiol. 2016 Aug; 32:120-7. PMID: 27315434; PMCID: PMC4983467 [Available on 08/01/17].
    3. Zhu L, Zhao J, Wang J, Hu C, Peng J, Luo R, Zhou C, Liu J, Lin J, Jin Y, Davis RE, Cheng G. MicroRNAs Are Involved in the Regulation of Ovary Development in the Pathogenic Blood Fluke Schistosoma japonicum. PLoS Pathog. 2016 Feb; 12(2):e1005423. PMID: 26871705; PMCID: PMC4752461.
    4. Neff AT, Wang J, Davis RE. "Father knows best?". EMBO J. 2014 Aug 18; 33(16):1729-31. PMID: 25061225; PMCID: PMC4195754.
    5. Wang J, Davis RE. Programmed DNA elimination in multicellular organisms. Curr Opin Genet Dev. 2014 Aug; 27:26-34. PMID: 24886889; PMCID: PMC4125452.
    6. Wang J, Davis RE. Contribution of transcription to animal early development. Transcription. 2014; 5(4):e967602. PMID: 25483604; PMCID: PMC4581351.
    7. Wang J, Garrey J, Davis RE. Transcription in pronuclei and one- to four-cell embryos drives early development in a nematode. Curr Biol. 2014 Jan 20; 24(2):124-33. PMID: 24374308; PMCID: PMC3953457.
    8. Wang J, Mitreva M, Berriman M, Thorne A, Magrini V, Koutsovoulos G, Kumar S, Blaxter ML, Davis RE. Silencing of germline-expressed genes by DNA elimination in somatic cells. Dev Cell. 2012 Nov 13; 23(5):1072-80. PMID: 23123092; PMCID: PMC3620533.
    9. Liu W, Jankowska-Anyszka M, Piecyk K, Dickson L, Wallace A, Niedzwiecka A, Stepinski J, Stolarski R, Darzynkiewicz E, Kieft J, Zhao R, Jones DN, Davis RE. Structural basis for nematode eIF4E binding an m(2,2,7)G-Cap and its implications for translation initiation. Nucleic Acids Res. 2011 Nov 01; 39(20):8820-32. PMID: 21965542; PMCID: PMC3203607.
    10. Wang J, Czech B, Crunk A, Wallace A, Mitreva M, Hannon GJ, Davis RE. Deep small RNA sequencing from the nematode Ascaris reveals conservation, functional diversification, and novel developmental profiles. Genome Res. 2011 Sep; 21(9):1462-77. PMID: 21685128; PMCID: PMC3166831.
    11. Wallace A, Filbin ME, Veo B, McFarland C, Stepinski J, Jankowska-Anyszka M, Darzynkiewicz E, Davis RE. The nematode eukaryotic translation initiation factor 4E/G complex works with a trans-spliced leader stem-loop to enable efficient translation of trimethylguanosine-capped RNAs. Mol Cell Biol. 2010 Apr; 30(8):1958-70. PMID: 20154140; PMCID: PMC2849463.
    12. Liu W, Zhao R, McFarland C, Kieft J, Niedzwiecka A, Jankowska-Anyszka M, Stepinski J, Darzynkiewicz E, Jones DN, Davis RE. Structural insights into parasite eIF4E binding specificity for m7G and m2,2,7G mRNA caps. J Biol Chem. 2009 Nov 06; 284(45):31336-49. PMID: 19710013; PMCID: PMC2781531.
    13. Cheng G, Davis RE. An improved and secreted luciferase reporter for schistosomes. Mol Biochem Parasitol. 2007 Oct; 155(2):167-71. PMID: 17681388; PMCID: PMC3641815.
    14. Cheng G, Cohen L, Mikhli C, Jankowska-Anyszka M, Stepinski J, Darzynkiewicz E, Davis RE. In vivo translation and stability of trans-spliced mRNAs in nematode embryos. Mol Biochem Parasitol. 2007 Jun; 153(2):95-106. PMID: 17391777; PMCID: PMC3650844.
    15. Cheng G, Cohen L, Ndegwa D, Davis RE. The flatworm spliced leader 3'-terminal AUG as a translation initiator methionine. J Biol Chem. 2006 Jan 13; 281(2):733-43. PMID: 16230357.
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