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Robert A Sclafani

TitleProfessor
InstitutionUniversity of Colorado Denver - Anschutz Medical Campus
DepartmentSOM-BioChem&Molecular Genetics
Phone303/724-3271

    Collapse Research 
    Collapse research activities and funding
    R01GM035078     (SCLAFANI, ROBERT A)Sep 6, 1985 - Mar 31, 2014
    NIH/NIGMS
    Genetic and Molecular Analysis of Yeast DNA Replication
    Role: Principal Investigator

    T32GM008730     (SCLAFANI, ROBERT A)Jul 1, 1999 - Jun 30, 2020
    NIH/NIGMS
    Predoctoral Training Program in Molecular Biology
    Role: Principal Investigator

    Collapse Bibliographic 
    Collapse selected publications
    Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Faculty can login to make corrections and additions.
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    1. Rossbach D, Bryan DS, Hesselberth JR, Sclafani R. Localization of Cdc7 Protein Kinase During DNA Replication in Saccharomyces cerevisiae. G3 (Bethesda). 2017 Sep 18. PMID: 28924058.
      View in: PubMed
    2. Todd MC, Langan TA, Sclafani RA. Doxycycline-Regulated p16MTS1 Expression Suppresses the Anchorage-Independence and Tumorigenicity of Breast Cancer Cell Lines that Lack Endogenous p16. J Cancer. 2017; 8(2):190-198. PMID: 28243323.
      View in: PubMed
    3. An X, Zhang C, Sclafani RA, Seligman P, Huang M. The late-annotated small ORF LSO1 is a target gene of the iron regulon of Saccharomyces cerevisiae. Microbiologyopen. 2015 Dec; 4(6):941-51. PMID: 26450372; PMCID: PMC4694146.
    4. Shrotriya S, Agarwal R, Sclafani RA. A perspective on chemoprevention by resveratrol in head and neck squamous cell carcinoma. Adv Exp Med Biol. 2015; 815:333-48. PMID: 25427916.
      View in: PubMed
    5. Ramey CJ, Sclafani RA. Functional conservation of the pre-sensor one beta-finger hairpin (PS1-hp) structures in mini-chromosome maintenance proteins of Saccharomyces cerevisiae and archaea. G3 (Bethesda). 2014 May 28; 4(7):1319-26. PMID: 24875627; PMCID: PMC4455780.
    6. Brandão LN, Ferguson R, Santoro I, Jinks-Robertson S, Sclafani RA. The role of Dbf4-dependent protein kinase in DNA polymerase ?-dependent mutagenesis in Saccharomyces cerevisiae. Genetics. 2014 Aug; 197(4):1111-22. PMID: 24875188; PMCID: PMC4125387.
    7. Shrotriya S, Tyagi A, Deep G, Orlicky DJ, Wisell J, Wang XJ, Sclafani RA, Agarwal R, Agarwal C. Grape seed extract and resveratrol prevent 4-nitroquinoline 1-oxide induced oral tumorigenesis in mice by modulating AMPK activation and associated biological responses. Mol Carcinog. 2015 Apr; 54(4):291-300. PMID: 24243690; PMCID: PMC4206677.
    8. Zhong Y, Nellimoottil T, Peace JM, Knott SR, Villwock SK, Yee JM, Jancuska JM, Rege S, Tecklenburg M, Sclafani RA, Tavaré S, Aparicio OM. The level of origin firing inversely affects the rate of replication fork progression. J Cell Biol. 2013 Apr 29; 201(3):373-83. PMID: 23629964; PMCID: PMC3639389.
    9. Han KJ, Foster DG, Zhang NY, Kanisha K, Dzieciatkowska M, Sclafani RA, Hansen KC, Peng J, Liu CW. Ubiquitin-specific protease 9x deubiquitinates and stabilizes the spinal muscular atrophy protein-survival motor neuron. J Biol Chem. 2012 Dec 21; 287(52):43741-52. PMID: 23112048; PMCID: PMC3527959.
    10. Bahmed K, Henry C, Holliday M, Redzic J, Ciobanu M, Zhang F, Weekes C, Sclafani R, Degregori J, Eisenmesser E. Extracellular cyclophilin-A stimulates ERK1/2 phosphorylation in a cell-dependent manner but broadly stimulates nuclear factor kappa B. Cancer Cell Int. 2012 Jul 04; 12(1):19. PMID: 22631225; PMCID: PMC3390265.
    11. Tyagi A, Gu M, Takahata T, Frederick B, Agarwal C, Siriwardana S, Agarwal R, Sclafani RA. Resveratrol selectively induces DNA Damage, independent of Smad4 expression, in its efficacy against human head and neck squamous cell carcinoma. Clin Cancer Res. 2011 Aug 15; 17(16):5402-11. PMID: 21705453; PMCID: PMC3156958.
    12. Radhakrishnan S, Reddivari L, Sclafani R, Das UN, Vanamala J. Resveratrol potentiates grape seed extract induced human colon cancer cell apoptosis. Front Biosci (Elite Ed). 2011 Jun 01; 3:1509-23. PMID: 21622155.
      View in: PubMed
    13. Kaur M, Tyagi A, Singh RP, Sclafani RA, Agarwal R, Agarwal C. Grape seed extract upregulates p21 (Cip1) through redox-mediated activation of ERK1/2 and posttranscriptional regulation leading to cell cycle arrest in colon carcinoma HT29 cells. Mol Carcinog. 2011 Jul; 50(7):553-62. PMID: 21268136; PMCID: PMC3110540.
    14. Holzen TM, Sclafani R. Genetic interaction of RAD53 protein kinase with histones is important for DNA replication. Cell Cycle. 2010 Dec 01; 9(23):4735-47. PMID: 21099362; PMCID: PMC3048039.
    15. Chien CY, Chen BR, Chou CK, Sclafani RA, Su JY. The yeast Cdc8 exhibits both deoxythymidine monophosphate and diphosphate kinase activities. FEBS Lett. 2009 Jul 07; 583(13):2281-6. PMID: 19540237; PMCID: PMC4084553.
    16. Roy S, Gu M, Ramasamy K, Singh RP, Agarwal C, Siriwardana S, Sclafani RA, Agarwal R. p21/Cip1 and p27/Kip1 Are essential molecular targets of inositol hexaphosphate for its antitumor efficacy against prostate cancer. Cancer Res. 2009 Feb 01; 69(3):1166-73. PMID: 19176374; PMCID: PMC2633420.
    17. Leon RP, Tecklenburg M, Sclafani RA. Functional conservation of beta-hairpin DNA binding domains in the Mcm protein of Methanobacterium thermoautotrophicum and the Mcm5 protein of Saccharomyces cerevisiae. Genetics. 2008 Aug; 179(4):1757-68. PMID: 18660534; PMCID: PMC2516056.
    18. Roy S, Singh RP, Agarwal C, Siriwardana S, Sclafani R, Agarwal R. Downregulation of both p21/Cip1 and p27/Kip1 produces a more aggressive prostate cancer phenotype. Cell Cycle. 2008 Jun 15; 7(12):1828-35. PMID: 18583941; PMCID: PMC2744498.
    19. Roy S, Kaur M, Agarwal C, Tecklenburg M, Sclafani RA, Agarwal R. p21 and p27 induction by silibinin is essential for its cell cycle arrest effect in prostate carcinoma cells. Mol Cancer Ther. 2007 Oct; 6(10):2696-707. PMID: 17938263.
      View in: PubMed
    20. Hoang ML, Leon RP, Pessoa-Brandao L, Hunt S, Raghuraman MK, Fangman WL, Brewer BJ, Sclafani RA. Structural changes in Mcm5 protein bypass Cdc7-Dbf4 function and reduce replication origin efficiency in Saccharomyces cerevisiae. Mol Cell Biol. 2007 Nov; 27(21):7594-602. PMID: 17724082; PMCID: PMC2169039.
    21. Sclafani RA, Holzen TM. Cell cycle regulation of DNA replication. Annu Rev Genet. 2007; 41:237-80. PMID: 17630848; PMCID: PMC2292467.
    22. Dohrmann PR, Sclafani RA. Novel role for checkpoint Rad53 protein kinase in the initiation of chromosomal DNA replication in Saccharomyces cerevisiae. Genetics. 2006 Sep; 174(1):87-99. PMID: 16816422; PMCID: PMC1569810.
    23. Tyagi A, Singh RP, Agarwal C, Siriwardana S, Sclafani RA, Agarwal R. Resveratrol causes Cdc2-tyr15 phosphorylation via ATM/ATR-Chk1/2-Cdc25C pathway as a central mechanism for S phase arrest in human ovarian carcinoma Ovcar-3 cells. Carcinogenesis. 2005 Nov; 26(11):1978-87. PMID: 15975956.
      View in: PubMed
    24. Sclafani RA, Fletcher RJ, Chen XS. Two heads are better than one: regulation of DNA replication by hexameric helicases. Genes Dev. 2004 Sep 01; 18(17):2039-45. PMID: 15342486; PMCID: PMC2292464.
    25. Pessoa-Brandão L, Sclafani RA. CDC7/DBF4 functions in the translesion synthesis branch of the RAD6 epistasis group in Saccharomyces cerevisiae. Genetics. 2004 Aug; 167(4):1597-610. PMID: 15342501; PMCID: PMC1471023.
    26. Agarwal C, Singh RP, Dhanalakshmi S, Tyagi AK, Tecklenburg M, Sclafani RA, Agarwal R. Silibinin upregulates the expression of cyclin-dependent kinase inhibitors and causes cell cycle arrest and apoptosis in human colon carcinoma HT-29 cells. Oncogene. 2003 Nov 13; 22(51):8271-82. PMID: 14614451.
      View in: PubMed
    27. Limesand KH, Barzen KA, Sanders LA, Sclafani RA, Raynolds MV, Reyland ME, Anderson SM, Quissell DO. Characterization of rat parotid and submandibular acinar cell apoptosis in primary culture. In Vitro Cell Dev Biol Anim. 2003 Mar-Apr; 39(3-4):170-7. PMID: 14505429; PMCID: PMC2879403.
    28. Fletcher RJ, Bishop BE, Leon RP, Sclafani RA, Ogata CM, Chen XS. The structure and function of MCM from archaeal M. Thermoautotrophicum. Nat Struct Biol. 2003 Mar; 10(3):160-7. PMID: 12548282.
      View in: PubMed
    29. Sclafani RA, Tecklenburg M, Pierce A. The mcm5-bob1 bypass of Cdc7p/Dbf4p in DNA replication depends on both Cdk1-independent and Cdk1-dependent steps in Saccharomyces cerevisiae. Genetics. 2002 May; 161(1):47-57. PMID: 12019222; PMCID: PMC1462111.
    30. Fouty BW, Grimison B, Fagan KA, Le Cras TD, Harral JW, Hoedt-Miller M, Sclafani RA, Rodman DM. p27(Kip1) is important in modulating pulmonary artery smooth muscle cell proliferation. Am J Respir Cell Mol Biol. 2001 Nov; 25(5):652-8. PMID: 11713109.
      View in: PubMed
    31. Mamay CL, Schauer IE, Rice PL, McDoniels-Silvers A, Dwyer-Nield LD, You M, Sclafani RA, Malkinson AM. Cyclin D1 as a proliferative marker regulating retinoblastoma phosphorylation in mouse lung epithelial cells. Cancer Lett. 2001 Jul 26; 168(2):165-72. PMID: 11403921.
      View in: PubMed
    32. Grimison B, Langan TA, Sclafani RA. P16Ink4a tumor suppressor function in lung cancer cells involves cyclin-dependent kinase 2 inhibition by Cip/Kip protein redistribution. Cell Growth Differ. 2000 Oct; 11(10):507-15. PMID: 11063124.
      View in: PubMed
    33. Sclafani RA. Cdc7p-Dbf4p becomes famous in the cell cycle. J Cell Sci. 2000 Jun; 113 ( Pt 12):2111-7. PMID: 10825284.
      View in: PubMed
    34. Todd MC, Sclafani RA, Langan TA. Ovarian cancer cells that coexpress endogenous Rb and p16 are insensitive to overexpression of functional p16 protein. Oncogene. 2000 Jan 13; 19(2):258-64. PMID: 10645004.
      View in: PubMed
    35. Oshiro G, Owens JC, Shellman Y, Sclafani RA, Li JJ. Cell cycle control of Cdc7p kinase activity through regulation of Dbf4p stability. Mol Cell Biol. 1999 Jul; 19(7):4888-96. PMID: 10373538; PMCID: PMC84289.
    36. Shellman YG, Svee E, Sclafani RA, Langan TA. Identification and characterization of individual cyclin-dependent kinase complexes from Saccharomyces cerevisiae. Yeast. 1999 Mar 15; 15(4):295-309. PMID: 10206189.
      View in: PubMed
    37. Dohrmann PR, Oshiro G, Tecklenburg M, Sclafani RA. RAD53 regulates DBF4 independently of checkpoint function in Saccharomyces cerevisiae. Genetics. 1999 Mar; 151(3):965-77. PMID: 10049915; PMCID: PMC1460535.
    38. Sclafani RA. Chromosomes in the Rocky Mountains. Yeast chromosome structure, replication and segregation, Snowmass, CO, USA, 8-13 August 1998. Trends Genet. 1998 Nov; 14(11):441-2. PMID: 9825670.
      View in: PubMed
    39. Shellman YG, Schauer IE, Oshiro G, Dohrmann P, Sclafani RA. Oligomers of the Cdc7/Dbf4 protein kinase exist in the yeast cell. Mol Gen Genet. 1998 Sep; 259(4):429-36. PMID: 9790600.
      View in: PubMed
    40. Hess GF, Drong RF, Weiland KL, Slightom JL, Sclafani RA, Hollingsworth RE. A human homolog of the yeast CDC7 gene is overexpressed in some tumors and transformed cell lines. Gene. 1998 Apr 28; 211(1):133-40. PMID: 9573348.
      View in: PubMed
    41. Groshong SD, Owen GI, Grimison B, Schauer IE, Todd MC, Langan TA, Sclafani RA, Lange CA, Horwitz KB. Biphasic regulation of breast cancer cell growth by progesterone: role of the cyclin-dependent kinase inhibitors, p21 and p27(Kip1). Mol Endocrinol. 1997 Oct; 11(11):1593-607. PMID: 9328342.
      View in: PubMed
    42. Hovland PG, Tecklenberg M, Sclafani RA. Overexpression of the protein kinase Pak1 suppresses yeast DNA polymerase mutations. Mol Gen Genet. 1997 Sep; 256(1):45-53. PMID: 9341678.
      View in: PubMed
    43. Hardy CF, Dryga O, Seematter S, Pahl PM, Sclafani RA. mcm5/cdc46-bob1 bypasses the requirement for the S phase activator Cdc7p. Proc Natl Acad Sci U S A. 1997 Apr 01; 94(7):3151-5. PMID: 9096361; PMCID: PMC20337.
    44. Sclafani RA. Cyclin dependent kinase activating kinases. Curr Opin Cell Biol. 1996 Dec; 8(6):788-94. PMID: 8939668.
      View in: PubMed
    45. Sclafani RA, Schauer IE. Cell cycle control and cancer: lessons from lung cancer. J Investig Dermatol Symp Proc. 1996 Apr; 1(2):123-7. PMID: 9627705.
      View in: PubMed
    46. Ostroff RM, Sclafani RA. Cell cycle regulation of induced mutagenesis in yeast. Mutat Res. 1995 Jul; 329(2):143-52. PMID: 7603496.
      View in: PubMed
    47. Schauer IE, Siriwardana S, Langan TA, Sclafani RA. Cyclin D1 overexpression vs. retinoblastoma inactivation: implications for growth control evasion in non-small cell and small cell lung cancer. Proc Natl Acad Sci U S A. 1994 Aug 02; 91(16):7827-31. PMID: 8052667; PMCID: PMC44495.
    48. Sclafani RA, Jackson AL. Cdc7 protein kinase for DNA metabolism comes of age. Mol Microbiol. 1994 Mar; 11(5):805-10. PMID: 8022258.
      View in: PubMed
    49. Hollingsworth RE, Sclafani RA. Yeast pre-meiotic DNA replication utilizes mitotic origin ARS1 independently of CDC7 function. Chromosoma. 1993 Jun; 102(6):415-20. PMID: 8365350.
      View in: PubMed
    50. Jackson AL, Pahl PM, Harrison K, Rosamond J, Sclafani RA. Cell cycle regulation of the yeast Cdc7 protein kinase by association with the Dbf4 protein. Mol Cell Biol. 1993 May; 13(5):2899-908. PMID: 8474449; PMCID: PMC359683.
    51. Hollingsworth RE, Ostroff RM, Klein MB, Niswander LA, Sclafani RA. Molecular genetic studies of the Cdc7 protein kinase and induced mutagenesis in yeast. Genetics. 1992 Sep; 132(1):53-62. PMID: 1398063; PMCID: PMC1205129.
    52. Su JY, Sclafani RA. Molecular cloning and expression of the human deoxythymidylate kinase gene in yeast. Nucleic Acids Res. 1991 Feb 25; 19(4):823-7. PMID: 2017365; PMCID: PMC333717.
    53. Hollingsworth RE, Sclafani RA. DNA metabolism gene CDC7 from yeast encodes a serine (threonine) protein kinase. Proc Natl Acad Sci U S A. 1990 Aug; 87(16):6272-6. PMID: 2166954; PMCID: PMC54515.
    54. Su JY, Belmont L, Sclafani RA. Genetic and molecular analysis of the SOE1 gene: a tRNA(3Glu) missense suppressor of yeast cdc8 mutations. Genetics. 1990 Mar; 124(3):523-31. PMID: 2155851; PMCID: PMC1203946.
    55. Hovland P, Flick J, Johnston M, Sclafani RA. Galactose as a gratuitous inducer of GAL gene expression in yeasts growing on glucose. Gene. 1989 Nov 15; 83(1):57-64. PMID: 2512199.
      View in: PubMed
    56. Langan TA, Gautier J, Lohka M, Hollingsworth R, Moreno S, Nurse P, Maller J, Sclafani RA. Mammalian growth-associated H1 histone kinase: a homolog of cdc2+/CDC28 protein kinases controlling mitotic entry in yeast and frog cells. Mol Cell Biol. 1989 Sep; 9(9):3860-8. PMID: 2550805; PMCID: PMC362447.
    57. Sclafani RA, Patterson M, Rosamond J, Fangman WL. Differential regulation of the yeast CDC7 gene during mitosis and meiosis. Mol Cell Biol. 1988 Jan; 8(1):293-300. PMID: 3275871; PMCID: PMC363120.
    58. Sclafani RA, Fangman WL. Thymidine utilization by tut mutants and facile cloning of mutant alleles by plasmid conversion in S. cerevisiae. Genetics. 1986 Nov; 114(3):753-67. PMID: 3025059; PMCID: PMC1203012.
    59. Patterson M, Sclafani RA, Fangman WL, Rosamond J. Molecular characterization of cell cycle gene CDC7 from Saccharomyces cerevisiae. Mol Cell Biol. 1986 May; 6(5):1590-8. PMID: 3537706; PMCID: PMC367685.
    60. Sclafani RA, Fangman WL. Yeast gene CDC8 encodes thymidylate kinase and is complemented by herpes thymidine kinase gene TK. Proc Natl Acad Sci U S A. 1984 Sep; 81(18):5821-5. PMID: 6091111; PMCID: PMC391803.
    61. Sclafani RA, Fangman WL. Conservative replication of double-stranded RNA in Saccharomyces cerevisiae by displacement of progeny single strands. Mol Cell Biol. 1984 Aug; 4(8):1618-26. PMID: 6387443; PMCID: PMC368955.
    62. Sclafani RA, Wechsler JA. High frequency of genetic duplications in the dnaB region of the Escherichia coli K12 chromosome. Genetics. 1981 Aug; 98(4):677-89. PMID: 7037536; PMCID: PMC1214468.
    63. Sclafani RA, Wechsler JA. Growth of phages lambda and phiX174 under Plban protein control in the absence of host dnaB function. Virology. 1981 Aug; 113(1):314-22. PMID: 6267785.
      View in: PubMed
    64. Sclafani RA, Wechsler JA. dnaB125, a dnaB nonsense mutation. J Bacteriol. 1981 Jun; 146(3):1170-3. PMID: 6263858; PMCID: PMC216976.
    65. Sclafani RA, Wechsler JA. Suppression of dnaC alleles by the dnaB analog (ban protein) of bacteriophage P1. J Bacteriol. 1981 Apr; 146(1):321-4. PMID: 7217002; PMCID: PMC217085.
    66. Sclafani RA, Wechsler JA. Deoxyribonucleic acid initiation mutation dnaB252 is suppressed by elevated dnaC+ gene dosage. J Bacteriol. 1981 Apr; 146(1):418-21. PMID: 7012124; PMCID: PMC217101.
    67. Sclafani RA, Wechsler JA. Deletion map of the Escherichia coli K-12 dnaB gene. Mol Gen Genet. 1981; 183(2):314-7. PMID: 7035829.
      View in: PubMed
    68. Sclafani RA, Wechsler JA, Schuster H. The isolation and characterization of escherichia coli dnaB::Tn10 insertion mutations. Mol Gen Genet. 1981; 182(1):112-8. PMID: 6267428.
      View in: PubMed
    69. Sclafani RA, Wechsler JA. DNA replication intermediates synthesized by lysates of dnaB, dnaG and dnaB dnaG mutants in vitro. Mol Gen Genet. 1981; 182(1):95-8. PMID: 6267435.
      View in: PubMed
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